Articles producció científica> Ciències Mèdiques Bàsiques

Evaluation of different conditions and culture media for the recovery of Aeromonas spp. from water and shellfish samples

  • Datos identificativos

    Identificador: imarina:6388786
    Autores:
    Latif-Eugenín FBeaz-Hidalgo RFigueras M
    Resumen:
    © 2016 The Society for Applied Microbiology Aims: To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples. Methods and Results: By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish. Conclusions: The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples. Significance and Impact of the Study: This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species.
  • Otros:

    Autor según el artículo: Latif-Eugenín F; Beaz-Hidalgo R; Figueras M
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: BEAZ HIDALGO, ROXANA / Figueras Salvat, María Josefa
    Palabras clave: Water Shellfish Enrichment Direct plating Culture media Aeromonas shellfish enrichment direct plating culture media aeromonas
    Resumen: © 2016 The Society for Applied Microbiology Aims: To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples. Methods and Results: By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P < 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P < 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P > 0·05). Isolation of Aeromonas media from water was favoured (P < 0·05) in the ADA medium, while SAA enhanced (P < 0·05) the isolation of Aer. salmonicida from shellfish. Conclusions: The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples. Significance and Impact of the Study: This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species.
    Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Engenharias i Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biotechnology Biodiversidade Astronomia / física Applied microbiology and biotechnology Administração pública e de empresas, ciências contábeis e turismo
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 13645072
    Direcció de correo del autor: mariajose.figueras@urv.cat
    Identificador del autor: 0000-0002-2268-8980
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/jam.13210
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Journal Of Applied Microbiology. 121 (3): 883-891
    Referencia de l'ítem segons les normes APA: Latif-Eugenín F; Beaz-Hidalgo R; Figueras M (2016). Evaluation of different conditions and culture media for the recovery of Aeromonas spp. from water and shellfish samples. Journal Of Applied Microbiology, 121(3), 883-891. DOI: 10.1111/jam.13210
    DOI del artículo: 10.1111/jam.13210
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Applied Microbiology and Biotechnology,Biotechnology,Biotechnology & Applied Microbiology,Medicine (Miscellaneous),Microbiology
    Water
    Shellfish
    Enrichment
    Direct plating
    Culture media
    Aeromonas
    shellfish
    enrichment
    direct plating
    culture media
    aeromonas
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrição
    Microbiology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Geociências
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Educação
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biotechnology & applied microbiology
    Biotechnology
    Biodiversidade
    Astronomia / física
    Applied microbiology and biotechnology
    Administração pública e de empresas, ciências contábeis e turismo
  • Documentos:

  • Cerca a google

    Search to google scholar