Author, as appears in the article.: Vinaixa, Maria; Rodriguez, Miguel A; Aivio, Suvi; Capellades, Jordi; Gomez, Josep; Canyellas, Nicolau; Stracker, Travis H; Yanes, Oscar
Department: Enginyeria Electrònica, Elèctrica i Automàtica
URV's Author/s: Cañellas Alberich, Nicolau / Gómez Alvarez, Josep / Rodríguez Gómez, Miquel Àngel / Vinaixa Crevillent, Maria / Yanes Torrado, Óscar
Keywords: Stable isotopes Spectra Quantification Protons Proton magnetic resonance spectroscopy Nmr spectroscopy Nmr Metabolomics Metabolome Metabolism Hyper-thyroid rats Humans High-throughput screening assays Glutamine-metabolism Gluconeogenesis Flux Extracts Database Cells Cell line, tumor Carbon isotopes nmr spectroscopy metabolomics metabolism
Abstract: © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C-satellite peaks using 1D-1H-NMR spectra. In comparison with 13C-NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high-throughput of 1H-NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D-NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
Thematic Areas: Química Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemistry Catalysis Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 14337851
Author's mail: josep.gomez@urv.cat maria.vinaixa@urv.cat oscar.yanes@urv.cat maria.vinaixa@urv.cat nicolau.canyellas@urv.cat
Author identifier: 0000-0002-0573-7621 0000-0001-9804-0171 0000-0003-3695-7157 0000-0001-9804-0171 0000-0003-4856-8132
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/anie.201611347
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Angewandte Chemie (International Ed. Print). 56 (13): 3531-3535
APA: Vinaixa, Maria; Rodriguez, Miguel A; Aivio, Suvi; Capellades, Jordi; Gomez, Josep; Canyellas, Nicolau; Stracker, Travis H; Yanes, Oscar (2017). Positional Enrichment by Proton Analysis (PEPA): A One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics. Angewandte Chemie (International Ed. Print), 56(13), 3531-3535. DOI: 10.1002/anie.201611347
Article's DOI: 10.1002/anie.201611347
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
Publication Type: Journal Publications
Repositori URV