Autor según el artículo: Vinaixa, Maria; Rodriguez, Miguel A; Aivio, Suvi; Capellades, Jordi; Gomez, Josep; Canyellas, Nicolau; Stracker, Travis H; Yanes, Oscar
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Cañellas Alberich, Nicolau / Gómez Alvarez, Josep / Rodríguez Gómez, Miquel Àngel / Vinaixa Crevillent, Maria / Yanes Torrado, Óscar
Palabras clave: Stable isotopes; Spectra; Quantification; Protons; Proton magnetic resonance spectroscopy; Nmr spectroscopy; Nmr; Metabolomics; Metabolome; Metabolism; Hyper-thyroid rats; Humans; High-throughput screening assays; Glutamine-metabolism; Gluconeogenesis; Flux; Extracts; Database; Cells; Cell line, tumor; Carbon isotopes; nmr spectroscopy; metabolomics; metabolism
Resumen: © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C-satellite peaks using 1D-1H-NMR spectra. In comparison with 13C-NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high-throughput of 1H-NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D-NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
Áreas temáticas: Química; Medicina ii; Medicina i; Materiais; Interdisciplinar; General medicine; General chemistry; Farmacia; Engenharias ii; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Chemistry, multidisciplinary; Chemistry (miscellaneous); Chemistry (all); Chemistry; Catalysis; Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 14337851
Direcció de correo del autor: josep.gomez@urv.cat; maria.vinaixa@urv.cat; oscar.yanes@urv.cat; maria.vinaixa@urv.cat; nicolau.canyellas@urv.cat
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/10.1002/anie.201611347
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Angewandte Chemie (International Ed. Print). 56 (13): 3531-3535
Referencia de l'ítem segons les normes APA: Vinaixa, Maria; Rodriguez, Miguel A; Aivio, Suvi; Capellades, Jordi; Gomez, Josep; Canyellas, Nicolau; Stracker, Travis H; Yanes, Oscar (2017). Positional Enrichment by Proton Analysis (PEPA): A One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics. Angewandte Chemie (International Ed. Print), 56(13), 3531-3535. DOI: 10.1002/anie.201611347
DOI del artículo: 10.1002/anie.201611347
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2017
Tipo de publicación: Journal Publications
Repositori URV