Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Positional Enrichment by Proton Analysis (PEPA): A One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics

  • Datos identificativos

    Identificador: imarina:6389030
    Autores:
    Vinaixa, MariaRodriguez, Miguel AAivio, SuviCapellades, JordiGomez, JosepCanyellas, NicolauStracker, Travis HYanes, Oscar
    Resumen:
    © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C-satellite peaks using 1D-1H-NMR spectra. In comparison with 13C-NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high-throughput of 1H-NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D-NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
  • Otros:

    Autor según el artículo: Vinaixa, Maria; Rodriguez, Miguel A; Aivio, Suvi; Capellades, Jordi; Gomez, Josep; Canyellas, Nicolau; Stracker, Travis H; Yanes, Oscar
    Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Cañellas Alberich, Nicolau / Gómez Alvarez, Josep / Rodríguez Gómez, Miquel Àngel / Vinaixa Crevillent, Maria / Yanes Torrado, Óscar
    Palabras clave: Stable isotopes Spectra Quantification Protons Proton magnetic resonance spectroscopy Nmr spectroscopy Nmr Metabolomics Metabolome Metabolism Hyper-thyroid rats Humans High-throughput screening assays Glutamine-metabolism Gluconeogenesis Flux Extracts Database Cells Cell line, tumor Carbon isotopes nmr spectroscopy metabolomics metabolism
    Resumen: © 2017 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. A novel metabolomics approach for NMR-based stable isotope tracer studies called PEPA is presented, and its performance validated using human cancer cells. PEPA detects the position of carbon label in isotopically enriched metabolites and quantifies fractional enrichment by indirect determination of 13C-satellite peaks using 1D-1H-NMR spectra. In comparison with 13C-NMR, TOCSY and HSQC, PEPA improves sensitivity, accelerates the elucidation of 13C positions in labeled metabolites and the quantification of the percentage of stable isotope enrichment. Altogether, PEPA provides a novel framework for extending the high-throughput of 1H-NMR metabolic profiling to stable isotope tracing in metabolomics, facilitating and complementing the information derived from 2D-NMR experiments and expanding the range of isotopically enriched metabolites detected in cellular extracts.
    Áreas temáticas: Química Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Chemistry Catalysis Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 14337851
    Direcció de correo del autor: josep.gomez@urv.cat maria.vinaixa@urv.cat oscar.yanes@urv.cat maria.vinaixa@urv.cat nicolau.canyellas@urv.cat
    Identificador del autor: 0000-0002-0573-7621 0000-0001-9804-0171 0000-0003-3695-7157 0000-0001-9804-0171 0000-0003-4856-8132
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Angewandte Chemie (International Ed. Print). 56 (13): 3531-3535
    Referencia de l'ítem segons les normes APA: Vinaixa, Maria; Rodriguez, Miguel A; Aivio, Suvi; Capellades, Jordi; Gomez, Josep; Canyellas, Nicolau; Stracker, Travis H; Yanes, Oscar (2017). Positional Enrichment by Proton Analysis (PEPA): A One-Dimensional 1H-NMR Approach for 13C Stable Isotope Tracer Studies in Metabolomics. Angewandte Chemie (International Ed. Print), 56(13), 3531-3535. DOI: 10.1002/anie.201611347
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2017
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Catalysis,Chemistry,Chemistry (Miscellaneous),Chemistry, Multidisciplinary
    Stable isotopes
    Spectra
    Quantification
    Protons
    Proton magnetic resonance spectroscopy
    Nmr spectroscopy
    Nmr
    Metabolomics
    Metabolome
    Metabolism
    Hyper-thyroid rats
    Humans
    High-throughput screening assays
    Glutamine-metabolism
    Gluconeogenesis
    Flux
    Extracts
    Database
    Cells
    Cell line, tumor
    Carbon isotopes
    nmr spectroscopy
    metabolomics
    metabolism
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    General medicine
    General chemistry
    Farmacia
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Chemistry
    Catalysis
    Astronomia / física
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