Author, as appears in the article.: Mas-Capdevila A; Iglesias-Carres L; Arola-Arnal A; Aragonès G; Muguerza B; Bravo FI
Department: Bioquímica i Biotecnologia
URV's Author/s: Aragonès Bargalló, Gerard / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Muguerza Marquínez, Maria Begoña
Keywords: Uhplc-ms/ms Uhplc-ms Uhplc-hrms Proteins Nitric oxide Naloxone Ms Caco-2 cells Blood-pressure Blood pressure Bioavailability Bioactive peptides Alpha-lactorphin uhplc-ms/ms uhplc-hrms naloxone caco-2 cells blood pressure bioactive peptides
Abstract: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption.
Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2218-273X
Author's mail: gerard.aragones@urv.cat franciscaisabel.bravo@urv.cat begona.muguerza@urv.cat anna.arola@urv.cat
Author identifier: 0000-0002-6468-3088 0000-0001-7384-8588 0000-0001-6529-1345
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2218-273X/10/7/992
Papper original source: Biomolecules. 10 (7): 1-12
APA: Mas-Capdevila A; Iglesias-Carres L; Arola-Arnal A; Aragonès G; Muguerza B; Bravo FI (2020). Implication of opioid receptors in the antihypertensive effect of a novel chicken foot-derived peptide. Biomolecules, 10(7), 1-12. DOI: 10.3390/biom10070992
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.3390/biom10070992
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV