Articles producció científica> Bioquímica i Biotecnologia

Implication of opioid receptors in the antihypertensive effect of a novel chicken foot-derived peptide

  • Datos identificativos

    Identificador: imarina:6685053
    Autores:
    Mas-Capdevila AIglesias-Carres LArola-Arnal AAragonès GMuguerza BBravo FI
    Resumen:
    © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption.
  • Otros:

    Autor según el artículo: Mas-Capdevila A; Iglesias-Carres L; Arola-Arnal A; Aragonès G; Muguerza B; Bravo FI
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: Aragonès Bargalló, Gerard / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Muguerza Marquínez, Maria Begoña
    Palabras clave: Uhplc-ms/ms Uhplc-ms Uhplc-hrms Proteins Nitric oxide Naloxone Ms Caco-2 cells Blood-pressure Blood pressure Bioavailability Bioactive peptides Alpha-lactorphin uhplc-ms/ms uhplc-hrms naloxone caco-2 cells blood pressure bioactive peptides
    Resumen: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. The peptide AVFQHNCQE demonstrated to produce nitric oxide-mediated antihypertensive effect. This study investigates the bioavailability and the opioid-like activity of this peptide after its oral administration. For this purpose, in silico and in vitro approaches were used to study the peptide susceptibility to GI digestion. In addition, AVFQHNCQE absorption was studied both in vitro by using Caco-2 cell monolayers and in vivo evaluating peptide presence in plasma from Wistar rats by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and by ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Both in vivo and in vitro experiments demonstrated that peptide AVFQHNCQE was not absorbed. Thus, the potential involvement of opioid receptors in the BP-lowering effect of AVFQHNCQE was studied in the presence of opioid receptors-antagonist Naloxone. No changes in blood pressure were recorded in rats administered Naloxone, demonstrating that AVFQHNCQE antihypertensive effect is mediated through its interaction with opioid receptors. AVFQHNCQE opioid-like activity would clarify the antihypertensive properties of AVFQHNCQE despite its lack of absorption.
    Áreas temáticas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 2218-273X
    Direcció de correo del autor: gerard.aragones@urv.cat franciscaisabel.bravo@urv.cat begona.muguerza@urv.cat anna.arola@urv.cat
    Identificador del autor: 0000-0002-6468-3088 0000-0001-7384-8588 0000-0001-6529-1345
    Fecha de alta del registro: 2023-02-19
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2218-273X/10/7/992
    Referencia al articulo segun fuente origial: Biomolecules. 10 (7): 1-12
    Referencia de l'ítem segons les normes APA: Mas-Capdevila A; Iglesias-Carres L; Arola-Arnal A; Aragonès G; Muguerza B; Bravo FI (2020). Implication of opioid receptors in the antihypertensive effect of a novel chicken foot-derived peptide. Biomolecules, 10(7), 1-12. DOI: 10.3390/biom10070992
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.3390/biom10070992
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry,Biochemistry & Molecular Biology,Molecular Biology
    Uhplc-ms/ms
    Uhplc-ms
    Uhplc-hrms
    Proteins
    Nitric oxide
    Naloxone
    Ms
    Caco-2 cells
    Blood-pressure
    Blood pressure
    Bioavailability
    Bioactive peptides
    Alpha-lactorphin
    uhplc-ms/ms
    uhplc-hrms
    naloxone
    caco-2 cells
    blood pressure
    bioactive peptides
    Química
    Molecular biology
    Materiais
    General medicine
    Farmacia
    Ensino
    Biochemistry & molecular biology
    Biochemistry
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