Articles producció científica> Medicina i Cirurgia

Hepatocyte Notch Signaling Deregulation Related to Lipid Metabolism in Women with Obesity and Nonalcoholic Fatty Liver

  • Identification data

    Identifier: imarina:7237759
    Authors:
    Auguet, TeresaBertran, LaiaBinetti, JessicaAguilar, CarmenMartinez, SalomeGuiu-Jurado, EstherSabench, FatimaAdalid, LaiaAntonio Porras, JoseRiesco, DavidDel Castillo, DanielRichart, Cristobal
    Abstract:
    Objective This cohort study aimed to explore the relationship between the Notch signaling pathway and the degree of nonalcoholic fatty liver disease (NAFLD). Moreover, this study intended to investigate whether this pathway is related to hepatic lipid metabolism and Toll-like receptors (TLRs). Methods This study used real-time polymerase chain reaction analysis to evaluate the hepatic expression level of all genes studied (Notch receptorsNOTCH1,NOTCH2,NOTCH3, andNOTCH4, transcription factorsHES1andHES5, and Hes-related repressor proteinsHEY1andHEY2) in hepatic tissue from two cohorts: women with severe obesity (n = 57) and normal liver structure (n = 20) or NAFLD (n = 37). Results In women with severe obesity and NAFLD, this study found downregulation of hepaticHES5expression. This expression correlated positively with the hepatic expression ofHES1,HEY1, andNOTCH3. This study also found a positive correlation betweenHES5expression and sterol regulatory element-binding protein 1c (SREBP1c) and betweenNOTCH3and several genes related to hepatic lipid metabolism (encoding liver X nuclear receptor alpha variant 1, farnesoid X nuclear receptor,SREBP1c, acetyl-CoA carboxylase 1, fatty acid synthase, peroxisome proliferator-activated receptor alpha, carnitine palmitoyltransferase 1, carnitineO-octanoyltransferase, ATP-binding cassette subfamily A member 1, and ATP-binding cassette subfamily G member 1). Finally, this study found a positive correlation betweenNOTCH2andTLR2,TLR4, andTLR9and a positive relationship betweenNOTCH1andTLR9. Conclusions Taken together, these findings suggest that hepatic expression of Notch proteins and ligands in relation to lipid metabolism pathways in the liver could have a role in NAFLD pathogenesis.
  • Others:

    Author, as appears in the article.: Auguet, Teresa; Bertran, Laia; Binetti, Jessica; Aguilar, Carmen; Martinez, Salome; Guiu-Jurado, Esther; Sabench, Fatima; Adalid, Laia; Antonio Porras, Jose; Riesco, David; Del Castillo, Daniel; Richart, Cristobal
    Department: Medicina i Cirurgia
    URV's Author/s: Adalid Llansà, Laia / Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Binetti, Jessica Angela / Del Castillo Déjardin, Daniel / GUIU JURADO, ESTHER / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima
    Keywords: Signal transduction Receptors, notch Obesity Non-alcoholic fatty liver disease Middle aged Lipid metabolism Insulin-resistance Inhibition Humans Hepatocytes Fibrosis Female Differentiation Cohort studies Animals Adaptive immunity
    Abstract: Objective This cohort study aimed to explore the relationship between the Notch signaling pathway and the degree of nonalcoholic fatty liver disease (NAFLD). Moreover, this study intended to investigate whether this pathway is related to hepatic lipid metabolism and Toll-like receptors (TLRs). Methods This study used real-time polymerase chain reaction analysis to evaluate the hepatic expression level of all genes studied (Notch receptorsNOTCH1,NOTCH2,NOTCH3, andNOTCH4, transcription factorsHES1andHES5, and Hes-related repressor proteinsHEY1andHEY2) in hepatic tissue from two cohorts: women with severe obesity (n = 57) and normal liver structure (n = 20) or NAFLD (n = 37). Results In women with severe obesity and NAFLD, this study found downregulation of hepaticHES5expression. This expression correlated positively with the hepatic expression ofHES1,HEY1, andNOTCH3. This study also found a positive correlation betweenHES5expression and sterol regulatory element-binding protein 1c (SREBP1c) and betweenNOTCH3and several genes related to hepatic lipid metabolism (encoding liver X nuclear receptor alpha variant 1, farnesoid X nuclear receptor,SREBP1c, acetyl-CoA carboxylase 1, fatty acid synthase, peroxisome proliferator-activated receptor alpha, carnitine palmitoyltransferase 1, carnitineO-octanoyltransferase, ATP-binding cassette subfamily A member 1, and ATP-binding cassette subfamily G member 1). Finally, this study found a positive correlation betweenNOTCH2andTLR2,TLR4, andTLR9and a positive relationship betweenNOTCH1andTLR9. Conclusions Taken together, these findings suggest that hepatic expression of Notch proteins and ligands in relation to lipid metabolism pathways in the liver could have a role in NAFLD pathogenesis.
    Thematic Areas: Saúde coletiva Psicología Nutrition and dietetics Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Endocrinology Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: joseantonio.porras@urv.cat mariasalome.martinez@urv.cat danieldel.castillo@urv.cat jessicaangela.binetti@estudiants.urv.cat laia.adalid@estudiants.urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Author identifier: 0000-0001-6418-1822 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-9660-7752 0000-0001-7543-9921 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Record's date: 2024-09-28
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/oby.22873
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Obesity. 28 (8): 1487-1493
    APA: Auguet, Teresa; Bertran, Laia; Binetti, Jessica; Aguilar, Carmen; Martinez, Salome; Guiu-Jurado, Esther; Sabench, Fatima; Adalid, Laia; Antonio Porras (2020). Hepatocyte Notch Signaling Deregulation Related to Lipid Metabolism in Women with Obesity and Nonalcoholic Fatty Liver. Obesity, 28(8), 1487-1493. DOI: 10.1002/oby.22873
    Article's DOI: 10.1002/oby.22873
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Endocrinology,Endocrinology & Metabolism,Endocrinology, Diabetes and Metabolism,Medicine (Miscellaneous),Nutrition & Dietetics,Nutrition and Dietetics
    Signal transduction
    Receptors, notch
    Obesity
    Non-alcoholic fatty liver disease
    Middle aged
    Lipid metabolism
    Insulin-resistance
    Inhibition
    Humans
    Hepatocytes
    Fibrosis
    Female
    Differentiation
    Cohort studies
    Animals
    Adaptive immunity
    Saúde coletiva
    Psicología
    Nutrition and dietetics
    Nutrition & dietetics
    Nutrição
    Medicine (miscellaneous)
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    General medicine
    Farmacia
    Enfermagem
    Endocrinology, diabetes and metabolism
    Endocrinology & metabolism
    Endocrinology
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciência de alimentos
    Biotecnología
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