Author, as appears in the article.: David Parada D; Peña G KB; Vives M; Molina A; Mayayo E; Riu F; Sabench F; del Castillo D

Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques

URV's Author/s: Del Castillo Déjardin, Daniel / Mayayo Artal, Emilio Manuel / MOLINA LÓPEZ, ALICIA / Sabench Pereferrer, Fàtima

Keywords: Serum Rats Obesity Laparoscopic surgery Laparoscopic sleeve gastrectomy Identification Hormone Histopathology Helicobacter-pylori infection Ghrelin Gastric Endocrine Cells Acylated peptide laparoscopic surgery ghrelin gastric cells

Abstract: © 2020 Parada D et al. Purpose: The cellular distribution of ghrelin positive cells is not well defined. The aim of the study is to quantify and determine the distribution of ghrelin cells in gastric mucosa in patients with morbid obesity and relate this finding to gender, comorbidities, gastritis, and plasmatic levels of acyl and desacylghrelin before and after laparoscopic sleeve gastrectomy. Patient and Methods: We performed a study on 61 patients with BMI≥50 kg/m2 operated by laparoscopic sleeve gastrectomy. Three gastric regions were analyzed by histopathology and immunohistology. Blood sample was taken before surgery, and at 6 and 12 months post-surgery, to study the plasma levels of ghrelin isoforms. Results: Ghrelin cells are present in all regions of the gastric mucosa, with a greater number in the body and the fundus. Difference was found in the antrum between male and female patients (p=0.018). Patients with arterial hypertension also showed a lower level of cells in antrum (p<0.05). Acylghrelin levels after surgery were significantly lower (32.83+5.6 pg/mL to 10.09+11.8 pg/mL, p<0.05). Values of desacylghrelin tended to decrease but no significant variation was observed (207.4+39.3 pg/mL to 188.84+52.3 pg/mL). Conclusion: Our patients show ghrelin cells in all areas of the stomach. Gender, comorbid-ities, and gastritis are determinant on gastric ghrelin-producing cells distribution.

Thematic Areas: Saúde coletiva Pharmacology Odontología Nutrição Medicina ii Medicina i Internal medicine Interdisciplinar Farmacia Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Biotecnología

licence for use: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 11787007

Author's mail: danieldel.castillo@urv.cat emilio.mayayo@urv.cat fatima.sabench@urv.cat

Author identifier: 0000-0003-0456-3102 0000-0001-7024-7824 0000-0003-4485-3809 0000-0002-9262-8756

Record's date: 2023-02-23

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443401/

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Diabetes Metabolic Syndrome And Obesity-Targets And Therapy. 13 2855-2864

APA: David Parada D; Peña G KB; Vives M; Molina A; Mayayo E; Riu F; Sabench F; del Castillo D (2020). Quantitative and topographic analysis by immunohistochemical expression of ghrelin gastric cells in patients with morbid obesity. Diabetes Metabolic Syndrome And Obesity-Targets And Therapy, 13(), 2855-2864. DOI: 10.2147/DMSO.S260483

Article's DOI: 10.2147/DMSO.S260483

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications