Articles producció científica> Medicina i Cirurgia

Quantitative and topographic analysis by immunohistochemical expression of ghrelin gastric cells in patients with morbid obesity

  • Identification data

    Identifier: imarina:7979989
    Authors:
    David Parada DPeña G KBVives MMolina AMayayo ERiu FSabench Fdel Castillo D
    Abstract:
    © 2020 Parada D et al. Purpose: The cellular distribution of ghrelin positive cells is not well defined. The aim of the study is to quantify and determine the distribution of ghrelin cells in gastric mucosa in patients with morbid obesity and relate this finding to gender, comorbidities, gastritis, and plasmatic levels of acyl and desacylghrelin before and after laparoscopic sleeve gastrectomy. Patient and Methods: We performed a study on 61 patients with BMI≥50 kg/m2 operated by laparoscopic sleeve gastrectomy. Three gastric regions were analyzed by histopathology and immunohistology. Blood sample was taken before surgery, and at 6 and 12 months post-surgery, to study the plasma levels of ghrelin isoforms. Results: Ghrelin cells are present in all regions of the gastric mucosa, with a greater number in the body and the fundus. Difference was found in the antrum between male and female patients (p=0.018). Patients with arterial hypertension also showed a lower level of cells in antrum (p<0.05). Acylghrelin levels after surgery were significantly lower (32.83+5.6 pg/mL to 10.09+11.8 pg/mL, p<0.05). Values of desacylghrelin tended to decrease but no significant variation was observed (207.4+39.3 pg/mL to 188.84+52.3 pg/mL). Conclusion: Our patients show ghrelin cells in all areas of the stomach. Gender, comorbid-ities, and gastritis are determinant on gastric ghrelin-producing cells distribution.
  • Others:

    Author, as appears in the article.: David Parada D; Peña G KB; Vives M; Molina A; Mayayo E; Riu F; Sabench F; del Castillo D
    Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques
    URV's Author/s: Del Castillo Déjardin, Daniel / Mayayo Artal, Emilio Manuel / MOLINA LÓPEZ, ALICIA / Sabench Pereferrer, Fàtima
    Keywords: Serum Rats Obesity Laparoscopic surgery Laparoscopic sleeve gastrectomy Identification Hormone Histopathology Helicobacter-pylori infection Ghrelin Gastric Endocrine Cells Acylated peptide laparoscopic surgery ghrelin gastric cells
    Abstract: © 2020 Parada D et al. Purpose: The cellular distribution of ghrelin positive cells is not well defined. The aim of the study is to quantify and determine the distribution of ghrelin cells in gastric mucosa in patients with morbid obesity and relate this finding to gender, comorbidities, gastritis, and plasmatic levels of acyl and desacylghrelin before and after laparoscopic sleeve gastrectomy. Patient and Methods: We performed a study on 61 patients with BMI≥50 kg/m2 operated by laparoscopic sleeve gastrectomy. Three gastric regions were analyzed by histopathology and immunohistology. Blood sample was taken before surgery, and at 6 and 12 months post-surgery, to study the plasma levels of ghrelin isoforms. Results: Ghrelin cells are present in all regions of the gastric mucosa, with a greater number in the body and the fundus. Difference was found in the antrum between male and female patients (p=0.018). Patients with arterial hypertension also showed a lower level of cells in antrum (p<0.05). Acylghrelin levels after surgery were significantly lower (32.83+5.6 pg/mL to 10.09+11.8 pg/mL, p<0.05). Values of desacylghrelin tended to decrease but no significant variation was observed (207.4+39.3 pg/mL to 188.84+52.3 pg/mL). Conclusion: Our patients show ghrelin cells in all areas of the stomach. Gender, comorbid-ities, and gastritis are determinant on gastric ghrelin-producing cells distribution.
    Thematic Areas: Saúde coletiva Pharmacology Odontología Nutrição Medicina ii Medicina i Internal medicine Interdisciplinar Farmacia Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 11787007
    Author's mail: danieldel.castillo@urv.cat emilio.mayayo@urv.cat fatima.sabench@urv.cat
    Author identifier: 0000-0003-0456-3102 0000-0001-7024-7824 0000-0003-4485-3809 0000-0002-9262-8756
    Record's date: 2023-02-23
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443401/
    Papper original source: Diabetes Metabolic Syndrome And Obesity-Targets And Therapy. 13 2855-2864
    APA: David Parada D; Peña G KB; Vives M; Molina A; Mayayo E; Riu F; Sabench F; del Castillo D (2020). Quantitative and topographic analysis by immunohistochemical expression of ghrelin gastric cells in patients with morbid obesity. Diabetes Metabolic Syndrome And Obesity-Targets And Therapy, 13(), 2855-2864. DOI: 10.2147/DMSO.S260483
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.2147/DMSO.S260483
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Endocrinology & Metabolism,Internal Medicine,Pharmacology
    Serum
    Rats
    Obesity
    Laparoscopic surgery
    Laparoscopic sleeve gastrectomy
    Identification
    Hormone
    Histopathology
    Helicobacter-pylori infection
    Ghrelin
    Gastric
    Endocrine
    Cells
    Acylated peptide
    laparoscopic surgery
    ghrelin
    gastric
    cells
    Saúde coletiva
    Pharmacology
    Odontología
    Nutrição
    Medicina ii
    Medicina i
    Internal medicine
    Interdisciplinar
    Farmacia
    Endocrinology & metabolism
    Educação física
    Ciências biológicas ii
    Ciências biológicas i
    Biotecnología
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