Author, as appears in the article.: Binetti J; Bertran L; Riesco D; Aguilar C; Martínez S; Sabench F; Porras JA; Camaron J; Del Castillo D; Richart C; Auguet T

Department: Medicina i Cirurgia

URV's Author/s: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Bertran Ramos, Laia / Binetti, Jessica Angela / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Porras Ledantes, Jose Antonio / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima

Keywords: Weight-loss Serotonin Plasma serotonin Peripheral serotonin Oxidative stress Non‐alcoholic fatty liver disease Non-alcoholic fatty liver disease Morbid obesity Metabolic syndrome Metabolic roles Gut microbiota Fatty liver-disease Expression Cell-function Bariatric surgery 5-ht4 receptors

Abstract: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Non‐alcoholic fatty liver disease (NAFLD) extends from simple steatosis (SS) to non‐alcoholic steatohepatitis (NASH). Peripheral serotonin (5‐HT) has become as an important regulator of different metabolic pathways. 5‐HT has been related to obesity and lipid accumulation in the liver. The objective of this study was to assess the relationship between the 5‐HT signaling pathway and the degree of NAFLD, as well as to investigate whether peripheral 5‐HT levels are related to the hepatic and jejunal mRNA abundance of serotonin receptors (HTR) in a cohort of women with morbid obesity (MO) and NAFLD. ELISA was used to quantify the serum 5‐HT from normal‐weight subjects (n = 26) and patients with MO (n = 58). We used RTq‐PCR analysis to evaluate the relative expression of HTR in women with MO with normal liver (n = 22), SS (n = 21), and NASH (n = 15). The 5‐HT was diminished in women with MO under a hypocaloric diet, regardless of the presence of NAFLD. Additionally, we report a negative correlation of 5‐HT levels with metabolic syndrome criteria, suggesting that serotonin may have a protective role in obesity. Additionally, the hepatic expression of HTR2A and HTR2B were decreased in women with MO and NAFLD, but no significant differences in the HTR jejunal expression according to the presence of NAFLD were found.

Thematic Areas: Space and planetary science Paleontology General biochemistry,genetics and molecular biology Ecology, evolution, behavior and systematics Biology Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: joseantonio.porras@urv.cat danieldel.castillo@urv.cat mariasalome.martinez@urv.cat cristobalmanuel.richart@urv.cat mariateresa.auguet@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat fatima.sabench@urv.cat laia.bertran@urv.cat jessicaangela.binetti@estudiants.urv.cat

Author identifier: 0000-0001-6418-1822 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-6185-2889 0000-0003-0852-6739 0000-0003-0396-6428 0000-0002-4440-562X 0000-0002-4440-562X 0000-0002-9262-8756 0000-0001-9052-1368 0000-0001-9660-7752

Record's date: 2023-06-24

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2075-1729/10/10/245

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Life. 10 (10): 1-17

APA: Binetti J; Bertran L; Riesco D; Aguilar C; Martínez S; Sabench F; Porras JA; Camaron J; Del Castillo D; Richart C; Auguet T (2020). Deregulated serotonin pathway in women with morbid obesity and nafld. Life, 10(10), 1-17. DOI: 10.3390/life10100245

Article's DOI: 10.3390/life10100245

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications