Articles producció científica> Ciències Mèdiques Bàsiques

Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice

  • Identification data

    Identifier: imarina:9138895
    Authors:
    Areitio MMartin-Vicente AArbizu AAntoran AAparicio-Fernandez LBuldain IMartin-Souto LRementeria ACapilla JHernando FLRamirez-Garcia A
    Abstract:
    © 2020 Asociación Española de Micología Background: Mucor circinelloides is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment. Aims: In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of M. circinelloides using sera from immunocompromised infected mice. Methods: The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of M. circinelloides. Results: Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract. Conclusions: In this work the most immunoreactive antigens of the secretome and the total extract of M. circinelloides were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.
  • Others:

    Author, as appears in the article.: Areitio M; Martin-Vicente A; Arbizu A; Antoran A; Aparicio-Fernandez L; Buldain I; Martin-Souto L; Rementeria A; Capilla J; Hernando FL; Ramirez-Garcia A
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Capilla Luque, Javier
    Keywords: Serological antigen Mucormycosis Immunoreactive protein Diagnostic target Antigen detection Antigen
    Abstract: © 2020 Asociación Española de Micología Background: Mucor circinelloides is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment. Aims: In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of M. circinelloides using sera from immunocompromised infected mice. Methods: The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of M. circinelloides. Results: Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract. Conclusions: In this work the most immunoreactive antigens of the secretome and the total extract of M. circinelloides were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.
    Thematic Areas: Saúde coletiva Revistas de ciencias de la naturaleza Odontología Mycology Microbiology Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases Farmacia Engenharias ii Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: javier.capilla@urv.cat
    Author identifier: 0000-0002-0765-6403
    Record's date: 2023-02-19
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1130140620300322?via%3Dihub
    Papper original source: Revista Iberoamericana De Micologia. 37 (3-4): 81-86
    APA: Areitio M; Martin-Vicente A; Arbizu A; Antoran A; Aparicio-Fernandez L; Buldain I; Martin-Souto L; Rementeria A; Capilla J; Hernando FL; Ramirez-Garci (2020). Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice. Revista Iberoamericana De Micologia, 37(3-4), 81-86. DOI: 10.1016/j.riam.2020.07.002
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.riam.2020.07.002
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2020
    Publication Type: Journal Publications
  • Keywords:

    Infectious Diseases,Microbiology,Mycology
    Serological antigen
    Mucormycosis
    Immunoreactive protein
    Diagnostic target
    Antigen detection
    Antigen
    Saúde coletiva
    Revistas de ciencias de la naturaleza
    Odontología
    Mycology
    Microbiology
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Infectious diseases
    Farmacia
    Engenharias ii
    Engenharias i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Biotecnología
    Biodiversidade
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