Author, as appears in the article.: Areitio M; Martin-Vicente A; Arbizu A; Antoran A; Aparicio-Fernandez L; Buldain I; Martin-Souto L; Rementeria A; Capilla J; Hernando FL; Ramirez-Garcia A

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Capilla Luque, Javier

Keywords: Serological antigen Mucormycosis Immunoreactive protein Diagnostic target Antigen detection Antigen

Abstract: © 2020 Asociación Española de Micología Background: Mucor circinelloides is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment. Aims: In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of M. circinelloides using sera from immunocompromised infected mice. Methods: The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of M. circinelloides. Results: Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract. Conclusions: In this work the most immunoreactive antigens of the secretome and the total extract of M. circinelloides were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.

Thematic Areas: Saúde coletiva Revistas de ciencias de la naturaleza Odontología Mycology Microbiology Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases Farmacia Engenharias ii Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: javier.capilla@urv.cat

Author identifier: 0000-0002-0765-6403

Record's date: 2023-02-19

Papper version: info:eu-repo/semantics/acceptedVersion

Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1130140620300322?via%3Dihub

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Revista Iberoamericana De Micologia. 37 (3-4): 81-86

APA: Areitio M; Martin-Vicente A; Arbizu A; Antoran A; Aparicio-Fernandez L; Buldain I; Martin-Souto L; Rementeria A; Capilla J; Hernando FL; Ramirez-Garci (2020). Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice. Revista Iberoamericana De Micologia, 37(3-4), 81-86. DOI: 10.1016/j.riam.2020.07.002

Article's DOI: 10.1016/j.riam.2020.07.002

Entity: Universitat Rovira i Virgili

Journal publication year: 2020

Publication Type: Journal Publications