Autor según el artículo: Areitio M; Martin-Vicente A; Arbizu A; Antoran A; Aparicio-Fernandez L; Buldain I; Martin-Souto L; Rementeria A; Capilla J; Hernando FL; Ramirez-Garcia A

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: Capilla Luque, Javier

Palabras clave: Serological antigen Mucormycosis Immunoreactive protein Diagnostic target Antigen detection Antigen

Resumen: © 2020 Asociación Española de Micología Background: Mucor circinelloides is an opportunistic fungus capable of causing mucormycosis, a highly aggressive infection of quick spreading. Besides, it also has a high mortality rate due to late diagnosis and difficult treatment. Aims: In this study we have identified the most immunoreactive proteins of the secretome and the total protein extract of M. circinelloides using sera from immunocompromised infected mice. Methods: The proteins of the secretome and the total extract were analyzed by two-dimensional electrophoresis and the most immunoreactive antigens were detected by Western Blot, facing the sera of immunocompromised infected mice to the proteins obtained in both extracts of M. circinelloides. Results: Seven antigens were detected in the secretome extract, and two in the total extract, all of them corresponding only to three proteins. The enzyme enolase was detected in both extracts, while triosephosphate isomerase was detected in the secretome, and heat shock protein HSS1 in the total extract. Conclusions: In this work the most immunoreactive antigens of the secretome and the total extract of M. circinelloides were identified. The identified proteins are well known fungal antigens and, therefore, these findings can be useful for future research into alternatives for the diagnosis and treatment of mucormycosis.

Áreas temáticas: Saúde coletiva Revistas de ciencias de la naturaleza Odontología Mycology Microbiology Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases Farmacia Engenharias ii Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: javier.capilla@urv.cat

Identificador del autor: 0000-0002-0765-6403

Fecha de alta del registro: 2023-02-19

Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion

Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S1130140620300322?via%3Dihub

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Revista Iberoamericana De Micologia. 37 (3-4): 81-86

Referencia de l'ítem segons les normes APA: Areitio M; Martin-Vicente A; Arbizu A; Antoran A; Aparicio-Fernandez L; Buldain I; Martin-Souto L; Rementeria A; Capilla J; Hernando FL; Ramirez-Garci (2020). Identification of Mucor circinelloides antigens recognized by sera from immunocompromised infected mice. Revista Iberoamericana De Micologia, 37(3-4), 81-86. DOI: 10.1016/j.riam.2020.07.002

DOI del artículo: 10.1016/j.riam.2020.07.002

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2020

Tipo de publicación: Journal Publications