Author, as appears in the article.: Mari-Almirall, Marta; Cosgaya, Clara; Pitart, Cristina; Vines, Joaquim; Munoz, Laura; Campo, Irene; Cusco, Anna; Rodriguez-Serna, Laura; Santana, Gemina; Del Rio, Ana; Francino, Olga; Ciruela, Pilar; Pujol, Isabel; Ballester, Frederic; Marco, Francesc; Antonio Martinez, Jose; Soriano, Alex; Vila, Jordi; Roca, Ignasi
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Gomez Bertomeu, Frederic-Francesc / Pujol Bajador, Isabel
Keywords: Tobramycin; Tigecycline; Tertiary care center; Spain; Southern blotting; Sequence analysis; Sanger sequencing; R factor; Plasmids; Plasmid; Nonhuman; Nanopore sequencing; Multilocus sequence typing; Molecular typing; Minimum inhibitory concentration; Microbial sensitivity tests; Microbial sensitivity test; Meropenem; Levofloxacin; Klebsiella pneumoniae; Klebsiella infections; Klebsiella infection; Imipenem; Humans; Human; Health care facility; Health care delivery; Gentamicin; Genetics; Genetic resistance; Fosfomycin; Escherichia coli; Enzyme synthesis; Disk diffusion; Delivery of health care; Controlled study; Colistin; Clone cells; Clone; Clonal variation; Ciprofloxacin; Cell clone; Ceftazidime; Cefoxitin; Cefotaxime; Cefepime; Catalonia; Carbapenemase; Carbapenem resistant klebsiella pneumoniae; Carbapenem resistant escherichia coli; Broth dilution; Beta-lactamases; Beta lactamase; Bacterium isolate; Bacterium identification; Bacterial transmission; Aztreonam; Article; Antiinfective agent; Antibiotic sensitivity; Antibiotic resistome; Antibiotic agent; Anti-bacterial agents; Amikacin
Abstract: Objectives: To characterize the clonal spread of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli isolates between different healthcare institutions in Catalonia, Spain.
Methods: Antimicrobial susceptibility was tested by disc diffusion. MICs were determined by gradient diffusion or broth microdilution. Carbapenemase production was confirmed by Lateral flow. PCR and Sanger sequencing were used to identify the allelic variants of resistance genes. Clonality studies were performed by PFGE and MLST. Plasmid typing, conjugation assays, S1-PFGE plus Southern blotting and MinION Oxford Nanopore sequencing were used to characterize resistance plasmids.
Results: Twenty-nine carbapenem-resistant isolates recovered from three healthcare institutions between January and November 2016 were included: 14 K. pneumoniae isolates from a tertiary hospital in the south of Catalonia (hospital A); 2 K. pneumoniae isolates from a nearby healthcare centre; and 12 K. pneumoniae isolates and 1 E. coli isolate from a tertiary hospital in Barcelona (hospital B). The majority of isolates were resistant to all antimicrobial agents, except colistin, and all were NDM producers. PFGE identified a major K. pneumoniae clone (n = 27) belonging to ST147 and co-producing NDM-1 and CTX-M-15, with a few isolates also harbouring bla(OXA-48). Two sporadic isolates of K. pneumoniae ST307 and E. coli ST167 producing NDM-7 were also identified. bla(OXA-48). was carried in two related IncR plasmid populations and bla(NDM)(-1) in a conjugative 50 kb IncX3 plasmid.
Conclusions: We report the inter-hospital dissemination of XDR high-risk clones of K. pneumoniae and E. coli associated with the carriage of small, transferable plasmids harbouring bla(NDM) genes.
Thematic Areas: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Pharmacology (medical); Pharmacology & pharmacy; Pharmacology; Odontología; Nutrição; Microbiology (medical); Microbiology; Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Infectious diseases; General medicine; Farmacia; Engenharias iii; Engenharias ii; Enfermagem; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciência de alimentos; Biotecnología; Biodiversidade; Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: frederic-francesc.gomez@urv.cat; isabel.pujol@urv.cat
Record's date: 2025-02-24
Paper version: info:eu-repo/semantics/submittedVersion
Link to the original source: https://academic.oup.com/jac/article/76/2/345/5983892?login=false
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Journal Of Antimicrobial Chemotherapy. 76 (2): 345-354
APA: Mari-Almirall, Marta; Cosgaya, Clara; Pitart, Cristina; Vines, Joaquim; Munoz, Laura; Campo, Irene; Cusco, Anna; Rodriguez-Serna, Laura; Santana, Gemi (2021). Dissemination of NDM-producing Klebsiella pneumoniae and Escherichia coli high-risk clones in Catalan healthcare institutions. Journal Of Antimicrobial Chemotherapy, 76(2), 345-354. DOI: 10.1093/jac/dkaa459
Article's DOI: 10.1093/jac/dkaa459
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV