Autor según el artículo: Mari-Almirall, Marta; Cosgaya, Clara; Pitart, Cristina; Vines, Joaquim; Munoz, Laura; Campo, Irene; Cusco, Anna; Rodriguez-Serna, Laura; Santana, Gemina; Del Rio, Ana; Francino, Olga; Ciruela, Pilar; Pujol, Isabel; Ballester, Frederic; Marco, Francesc; Antonio Martinez, Jose; Soriano, Alex; Vila, Jordi; Roca, Ignasi;MERCyCAT Study Grp
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: Gomez Bertomeu, Frederic-Francesc / Pujol Bajador, Isabel
Palabras clave: Tobramycin Tigecycline Tertiary care center Spain Southern blotting Sequence analysis Sanger sequencing R factor Plasmids Plasmid Nonhuman Nanopore sequencing Multilocus sequence typing Molecular typing Minimum inhibitory concentration Microbial sensitivity tests Microbial sensitivity test Meropenem Levofloxacin Klebsiella pneumoniae Klebsiella infections Klebsiella infection Imipenem Humans Human Health care facility Health care delivery Gentamicin Genetics Genetic resistance Fosfomycin Escherichia coli Enzyme synthesis Disk diffusion Delivery of health care Controlled study Colistin Clone cells Clone Clonal variation Ciprofloxacin Cell clone Ceftazidime Cefoxitin Cefotaxime Cefepime Catalonia Carbapenemase Carbapenem resistant klebsiella pneumoniae Carbapenem resistant escherichia coli Broth dilution Beta-lactamases Beta lactamase Bacterium isolate Bacterium identification Bacterial transmission Aztreonam Article Antiinfective agent Antibiotic sensitivity Antibiotic resistome Antibiotic agent Anti-bacterial agents Amikacin
Resumen: Objectives: To characterize the clonal spread of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli isolates between different healthcare institutions in Catalonia, Spain.
Methods: Antimicrobial susceptibility was tested by disc diffusion. MICs were determined by gradient diffusion or broth microdilution. Carbapenemase production was confirmed by Lateral flow. PCR and Sanger sequencing were used to identify the allelic variants of resistance genes. Clonality studies were performed by PFGE and MLST. Plasmid typing, conjugation assays, S1-PFGE plus Southern blotting and MinION Oxford Nanopore sequencing were used to characterize resistance plasmids.
Results: Twenty-nine carbapenem-resistant isolates recovered from three healthcare institutions between January and November 2016 were included: 14 K. pneumoniae isolates from a tertiary hospital in the south of Catalonia (hospital A); 2 K. pneumoniae isolates from a nearby healthcare centre; and 12 K. pneumoniae isolates and 1 E. coli isolate from a tertiary hospital in Barcelona (hospital B). The majority of isolates were resistant to all antimicrobial agents, except colistin, and all were NDM producers. PFGE identified a major K. pneumoniae clone (n = 27) belonging to ST147 and co-producing NDM-1 and CTX-M-15, with a few isolates also harbouring bla(OXA-48). Two sporadic isolates of K. pneumoniae ST307 and E. coli ST167 producing NDM-7 were also identified. bla(OXA-48). was carried in two related IncR plasmid populations and bla(NDM)(-1) in a conjugative 50 kb IncX3 plasmid.
Conclusions: We report the inter-hospital dissemination of XDR high-risk clones of K. pneumoniae and E. coli associated with the carriage of small, transferable plasmids harbouring bla(NDM) genes.
Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Pharmacology (medical) Pharmacology & pharmacy Pharmacology Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases General medicine Farmacia Engenharias iii Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: frederic-francesc.gomez@urv.cat isabel.pujol@urv.cat
Identificador del autor: 0000-0002-8039-2889
Fecha de alta del registro: 2024-07-27
Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Journal Of Antimicrobial Chemotherapy. 76 (2): 345-354
Referencia de l'ítem segons les normes APA: Mari-Almirall, Marta; Cosgaya, Clara; Pitart, Cristina; Vines, Joaquim; Munoz, Laura; Campo, Irene; Cusco, Anna; Rodriguez-Serna, Laura; Santana, Gemi (2021). Dissemination of NDM-producing Klebsiella pneumoniae and Escherichia coli high-risk clones in Catalan healthcare institutions. Journal Of Antimicrobial Chemotherapy, 76(2), 345-354. DOI: 10.1093/jac/dkaa459
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2021
Tipo de publicación: Journal Publications
Repositori URV