Articles producció científica> Ciències Mèdiques Bàsiques

Dissemination of NDM-producing Klebsiella pneumoniae and Escherichia coli high-risk clones in Catalan healthcare institutions

  • Datos identificativos

    Identificador: imarina:9173249
    Autores:
    Mari-Almirall, MartaCosgaya, ClaraPitart, CristinaVines, JoaquimMunoz, LauraCampo, IreneCusco, AnnaRodriguez-Serna, LauraSantana, GeminaDel Rio, AnaFrancino, OlgaCiruela, PilarPujol, IsabelBallester, FredericMarco, FrancescAntonio Martinez, JoseSoriano, AlexVila, JordiRoca, IgnasiMERCyCAT Study Grp
    Resumen:
    Objectives: To characterize the clonal spread of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli isolates between different healthcare institutions in Catalonia, Spain. Methods: Antimicrobial susceptibility was tested by disc diffusion. MICs were determined by gradient diffusion or broth microdilution. Carbapenemase production was confirmed by Lateral flow. PCR and Sanger sequencing were used to identify the allelic variants of resistance genes. Clonality studies were performed by PFGE and MLST. Plasmid typing, conjugation assays, S1-PFGE plus Southern blotting and MinION Oxford Nanopore sequencing were used to characterize resistance plasmids. Results: Twenty-nine carbapenem-resistant isolates recovered from three healthcare institutions between January and November 2016 were included: 14 K. pneumoniae isolates from a tertiary hospital in the south of Catalonia (hospital A); 2 K. pneumoniae isolates from a nearby healthcare centre; and 12 K. pneumoniae isolates and 1 E. coli isolate from a tertiary hospital in Barcelona (hospital B). The majority of isolates were resistant to all antimicrobial agents, except colistin, and all were NDM producers. PFGE identified a major K. pneumoniae clone (n = 27) belonging to ST147 and co-producing NDM-1 and CTX-M-15, with a few isolates also harbouring bla(OXA-48). Two sporadic isolates of K. pneumoniae ST307 and E. coli ST167 producing NDM-7 were also identified. bla(OXA-48). was carried in two related IncR plasmid populations and bla(NDM)(-1) in a conjugative 50 kb IncX3 plasmid. Conclusions: We report the inter-hospital dissemination of XDR high-risk clones of K. pneumoniae and E. coli associated with the carriage of small, transferable plasmids harbouring bla(NDM) genes.
  • Otros:

    Autor según el artículo: Mari-Almirall, Marta; Cosgaya, Clara; Pitart, Cristina; Vines, Joaquim; Munoz, Laura; Campo, Irene; Cusco, Anna; Rodriguez-Serna, Laura; Santana, Gemina; Del Rio, Ana; Francino, Olga; Ciruela, Pilar; Pujol, Isabel; Ballester, Frederic; Marco, Francesc; Antonio Martinez, Jose; Soriano, Alex; Vila, Jordi; Roca, Ignasi;MERCyCAT Study Grp
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Gomez Bertomeu, Frederic-Francesc / Pujol Bajador, Isabel
    Palabras clave: Tobramycin Tigecycline Tertiary care center Spain Southern blotting Sequence analysis Sanger sequencing R factor Plasmids Plasmid Nonhuman Nanopore sequencing Multilocus sequence typing Molecular typing Minimum inhibitory concentration Microbial sensitivity tests Microbial sensitivity test Meropenem Levofloxacin Klebsiella pneumoniae Klebsiella infections Klebsiella infection Imipenem Humans Human Health care facility Health care delivery Gentamicin Genetics Genetic resistance Fosfomycin Escherichia coli Enzyme synthesis Disk diffusion Delivery of health care Controlled study Colistin Clone cells Clone Clonal variation Ciprofloxacin Cell clone Ceftazidime Cefoxitin Cefotaxime Cefepime Catalonia Carbapenemase Carbapenem resistant klebsiella pneumoniae Carbapenem resistant escherichia coli Broth dilution Beta-lactamases Beta lactamase Bacterium isolate Bacterium identification Bacterial transmission Aztreonam Article Antiinfective agent Antibiotic sensitivity Antibiotic resistome Antibiotic agent Anti-bacterial agents Amikacin
    Resumen: Objectives: To characterize the clonal spread of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli isolates between different healthcare institutions in Catalonia, Spain. Methods: Antimicrobial susceptibility was tested by disc diffusion. MICs were determined by gradient diffusion or broth microdilution. Carbapenemase production was confirmed by Lateral flow. PCR and Sanger sequencing were used to identify the allelic variants of resistance genes. Clonality studies were performed by PFGE and MLST. Plasmid typing, conjugation assays, S1-PFGE plus Southern blotting and MinION Oxford Nanopore sequencing were used to characterize resistance plasmids. Results: Twenty-nine carbapenem-resistant isolates recovered from three healthcare institutions between January and November 2016 were included: 14 K. pneumoniae isolates from a tertiary hospital in the south of Catalonia (hospital A); 2 K. pneumoniae isolates from a nearby healthcare centre; and 12 K. pneumoniae isolates and 1 E. coli isolate from a tertiary hospital in Barcelona (hospital B). The majority of isolates were resistant to all antimicrobial agents, except colistin, and all were NDM producers. PFGE identified a major K. pneumoniae clone (n = 27) belonging to ST147 and co-producing NDM-1 and CTX-M-15, with a few isolates also harbouring bla(OXA-48). Two sporadic isolates of K. pneumoniae ST307 and E. coli ST167 producing NDM-7 were also identified. bla(OXA-48). was carried in two related IncR plasmid populations and bla(NDM)(-1) in a conjugative 50 kb IncX3 plasmid. Conclusions: We report the inter-hospital dissemination of XDR high-risk clones of K. pneumoniae and E. coli associated with the carriage of small, transferable plasmids harbouring bla(NDM) genes.
    Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Pharmacology (medical) Pharmacology & pharmacy Pharmacology Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases General medicine Farmacia Engenharias iii Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciência de alimentos Biotecnología Biodiversidade Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: frederic-francesc.gomez@urv.cat isabel.pujol@urv.cat
    Identificador del autor: 0000-0002-8039-2889
    Fecha de alta del registro: 2024-07-27
    Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
    Enlace a la fuente original: https://academic.oup.com/jac/article/76/2/345/5983892?login=false
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Journal Of Antimicrobial Chemotherapy. 76 (2): 345-354
    Referencia de l'ítem segons les normes APA: Mari-Almirall, Marta; Cosgaya, Clara; Pitart, Cristina; Vines, Joaquim; Munoz, Laura; Campo, Irene; Cusco, Anna; Rodriguez-Serna, Laura; Santana, Gemi (2021). Dissemination of NDM-producing Klebsiella pneumoniae and Escherichia coli high-risk clones in Catalan healthcare institutions. Journal Of Antimicrobial Chemotherapy, 76(2), 345-354. DOI: 10.1093/jac/dkaa459
    DOI del artículo: 10.1093/jac/dkaa459
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Infectious Diseases,Microbiology,Microbiology (Medical),Pharmacology,Pharmacology & Pharmacy,Pharmacology (Medical)
    Tobramycin
    Tigecycline
    Tertiary care center
    Spain
    Southern blotting
    Sequence analysis
    Sanger sequencing
    R factor
    Plasmids
    Plasmid
    Nonhuman
    Nanopore sequencing
    Multilocus sequence typing
    Molecular typing
    Minimum inhibitory concentration
    Microbial sensitivity tests
    Microbial sensitivity test
    Meropenem
    Levofloxacin
    Klebsiella pneumoniae
    Klebsiella infections
    Klebsiella infection
    Imipenem
    Humans
    Human
    Health care facility
    Health care delivery
    Gentamicin
    Genetics
    Genetic resistance
    Fosfomycin
    Escherichia coli
    Enzyme synthesis
    Disk diffusion
    Delivery of health care
    Controlled study
    Colistin
    Clone cells
    Clone
    Clonal variation
    Ciprofloxacin
    Cell clone
    Ceftazidime
    Cefoxitin
    Cefotaxime
    Cefepime
    Catalonia
    Carbapenemase
    Carbapenem resistant klebsiella pneumoniae
    Carbapenem resistant escherichia coli
    Broth dilution
    Beta-lactamases
    Beta lactamase
    Bacterium isolate
    Bacterium identification
    Bacterial transmission
    Aztreonam
    Article
    Antiinfective agent
    Antibiotic sensitivity
    Antibiotic resistome
    Antibiotic agent
    Anti-bacterial agents
    Amikacin
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Pharmacology (medical)
    Pharmacology & pharmacy
    Pharmacology
    Odontología
    Nutrição
    Microbiology (medical)
    Microbiology
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    General medicine
    Farmacia
    Engenharias iii
    Engenharias ii
    Enfermagem
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciência de alimentos
    Biotecnología
    Biodiversidade
    Astronomia / física
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