Author, as appears in the article.: Hoch, Denise; Bachbauer, Martina; Poechlauer, Caroline; Algaba-Chueca, Francisco; Tandl, Veronika; Novakovic, Boris; Megia, Ana; Gauster, Martin; Saffery, Richard; Glasner, Andreas; Desoye, Gernot; Majali-Martinez, Alejandro;
Department: Medicina i Cirurgia
URV's Author/s: Megía Colet, Ana
Keywords: Trophoblasts Phosphorylation Oxygen Obesity Invasion Insulin Impact Identification Human placenta Growth First trimester Expression Early pregnancy Checkpoint Cell cycle Brca1
Abstract: In the first trimester of pregnancy, placental development involves a wide range of cellular processes. These include trophoblast proliferation, fusion, and differentiation, which are dependent on tight cell cycle control. The intrauterine environment affects placental development, which also includes the trophoblast cell cycle. In this work, we focus on maternal obesity to assess whether an altered intrauterine milieu modulates expression and protein levels of placental cell cycle regulators in early human pregnancy. For this purpose, we use first trimester placental tissue from lean and obese women (gestational week 5(+0)-11(+6), n = 58). Using a PCR panel, a cell cycle protein array, and STRING database analysis, we identify a network of cell cycle regulators increased by maternal obesity in which breast cancer 1 (BRCA1) is a central player. Immunostaining localizes BRCA1 predominantly to the villous and the extravillous cytotrophoblast. Obesity-driven BRCA1 upregulation is not able to be explained by DNA methylation (EPIC array) or by short-term treatment of chorionic villous explants at 2.5% oxygen with tumor necrosis factor alpha (TNF-alpha) (50 mg/mL), leptin (100 mg/mL), interleukin 6 (IL-6) (100 mg/mL), or high glucose (25 nM). Oxygen tension rises during the first trimester, but this change in vitro has no effect on BRCA1 (2.5% and 6.5% O-2). We conclude that maternal obesity affects placental cell cycle regulation and speculate this may alter placental development.
Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: ana.megia@urv.cat
Author identifier: 0000-0002-5101-9452
Record's date: 2023-02-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/1422-0067/21/2/468
Papper original source: International Journal Of Molecular Sciences. 21 (2):
APA: Hoch, Denise; Bachbauer, Martina; Poechlauer, Caroline; Algaba-Chueca, Francisco; Tandl, Veronika; Novakovic, Boris; Megia, Ana; Gauster, Martin; Saff (2020). Maternal Obesity Alters Placental Cell Cycle Regulators in the First Trimester of Human Pregnancy: New Insights for BRCA1. International Journal Of Molecular Sciences, 21(2), -. DOI: 10.3390/ijms21020468
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.3390/ijms21020468
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV