Articles producció científica> Medicina i Cirurgia

Maternal Obesity Alters Placental Cell Cycle Regulators in the First Trimester of Human Pregnancy: New Insights for BRCA1

  • Datos identificativos

    Identificador: imarina:9216878
    Autores:
    Hoch, DeniseBachbauer, MartinaPoechlauer, CarolineAlgaba-Chueca, FranciscoTandl, VeronikaNovakovic, BorisMegia, AnaGauster, MartinSaffery, RichardGlasner, AndreasDesoye, GernotMajali-Martinez, Alejandro
    Resumen:
    In the first trimester of pregnancy, placental development involves a wide range of cellular processes. These include trophoblast proliferation, fusion, and differentiation, which are dependent on tight cell cycle control. The intrauterine environment affects placental development, which also includes the trophoblast cell cycle. In this work, we focus on maternal obesity to assess whether an altered intrauterine milieu modulates expression and protein levels of placental cell cycle regulators in early human pregnancy. For this purpose, we use first trimester placental tissue from lean and obese women (gestational week 5(+0)-11(+6), n = 58). Using a PCR panel, a cell cycle protein array, and STRING database analysis, we identify a network of cell cycle regulators increased by maternal obesity in which breast cancer 1 (BRCA1) is a central player. Immunostaining localizes BRCA1 predominantly to the villous and the extravillous cytotrophoblast. Obesity-driven BRCA1 upregulation is not able to be explained by DNA methylation (EPIC array) or by short-term treatment of chorionic villous explants at 2.5% oxygen with tumor necrosis factor alpha (TNF-alpha) (50 mg/mL), leptin (100 mg/mL), interleukin 6 (IL-6) (100 mg/mL), or high glucose (25 nM). Oxygen tension rises during the first trimester, but this change in vitro has no effect on BRCA1 (2.5% and 6.5% O-2). We conclude that maternal obesity affects placental cell cycle regulation and speculate this may alter placental development.
  • Otros:

    Autor según el artículo: Hoch, Denise; Bachbauer, Martina; Poechlauer, Caroline; Algaba-Chueca, Francisco; Tandl, Veronika; Novakovic, Boris; Megia, Ana; Gauster, Martin; Saffery, Richard; Glasner, Andreas; Desoye, Gernot; Majali-Martinez, Alejandro;
    Departamento: Medicina i Cirurgia
    Autor/es de la URV: Megía Colet, Ana
    Palabras clave: Trophoblasts Phosphorylation Oxygen Obesity Invasion Insulin Impact Identification Human placenta Growth First trimester Expression Early pregnancy Checkpoint Cell cycle Brca1
    Resumen: In the first trimester of pregnancy, placental development involves a wide range of cellular processes. These include trophoblast proliferation, fusion, and differentiation, which are dependent on tight cell cycle control. The intrauterine environment affects placental development, which also includes the trophoblast cell cycle. In this work, we focus on maternal obesity to assess whether an altered intrauterine milieu modulates expression and protein levels of placental cell cycle regulators in early human pregnancy. For this purpose, we use first trimester placental tissue from lean and obese women (gestational week 5(+0)-11(+6), n = 58). Using a PCR panel, a cell cycle protein array, and STRING database analysis, we identify a network of cell cycle regulators increased by maternal obesity in which breast cancer 1 (BRCA1) is a central player. Immunostaining localizes BRCA1 predominantly to the villous and the extravillous cytotrophoblast. Obesity-driven BRCA1 upregulation is not able to be explained by DNA methylation (EPIC array) or by short-term treatment of chorionic villous explants at 2.5% oxygen with tumor necrosis factor alpha (TNF-alpha) (50 mg/mL), leptin (100 mg/mL), interleukin 6 (IL-6) (100 mg/mL), or high glucose (25 nM). Oxygen tension rises during the first trimester, but this change in vitro has no effect on BRCA1 (2.5% and 6.5% O-2). We conclude that maternal obesity affects placental cell cycle regulation and speculate this may alter placental development.
    Áreas temáticas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: ana.megia@urv.cat
    Identificador del autor: 0000-0002-5101-9452
    Fecha de alta del registro: 2023-02-19
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/1422-0067/21/2/468
    Referencia al articulo segun fuente origial: International Journal Of Molecular Sciences. 21 (2):
    Referencia de l'ítem segons les normes APA: Hoch, Denise; Bachbauer, Martina; Poechlauer, Caroline; Algaba-Chueca, Francisco; Tandl, Veronika; Novakovic, Boris; Megia, Ana; Gauster, Martin; Saff (2020). Maternal Obesity Alters Placental Cell Cycle Regulators in the First Trimester of Human Pregnancy: New Insights for BRCA1. International Journal Of Molecular Sciences, 21(2), -. DOI: 10.3390/ijms21020468
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.3390/ijms21020468
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Trophoblasts
    Phosphorylation
    Oxygen
    Obesity
    Invasion
    Insulin
    Impact
    Identification
    Human placenta
    Growth
    First trimester
    Expression
    Early pregnancy
    Checkpoint
    Cell cycle
    Brca1
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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