Autor según el artículo: Hoch, Denise; Bachbauer, Martina; Poechlauer, Caroline; Algaba-Chueca, Francisco; Tandl, Veronika; Novakovic, Boris; Megia, Ana; Gauster, Martin; Saffery, Richard; Glasner, Andreas; Desoye, Gernot; Majali-Martinez, Alejandro;
Departamento: Medicina i Cirurgia
Autor/es de la URV: Megía Colet, Ana
Palabras clave: Trophoblasts Phosphorylation Oxygen Obesity Invasion Insulin Impact Identification Human placenta Growth First trimester Expression Early pregnancy Checkpoint Cell cycle Brca1
Resumen: In the first trimester of pregnancy, placental development involves a wide range of cellular processes. These include trophoblast proliferation, fusion, and differentiation, which are dependent on tight cell cycle control. The intrauterine environment affects placental development, which also includes the trophoblast cell cycle. In this work, we focus on maternal obesity to assess whether an altered intrauterine milieu modulates expression and protein levels of placental cell cycle regulators in early human pregnancy. For this purpose, we use first trimester placental tissue from lean and obese women (gestational week 5(+0)-11(+6), n = 58). Using a PCR panel, a cell cycle protein array, and STRING database analysis, we identify a network of cell cycle regulators increased by maternal obesity in which breast cancer 1 (BRCA1) is a central player. Immunostaining localizes BRCA1 predominantly to the villous and the extravillous cytotrophoblast. Obesity-driven BRCA1 upregulation is not able to be explained by DNA methylation (EPIC array) or by short-term treatment of chorionic villous explants at 2.5% oxygen with tumor necrosis factor alpha (TNF-alpha) (50 mg/mL), leptin (100 mg/mL), interleukin 6 (IL-6) (100 mg/mL), or high glucose (25 nM). Oxygen tension rises during the first trimester, but this change in vitro has no effect on BRCA1 (2.5% and 6.5% O-2). We conclude that maternal obesity affects placental cell cycle regulation and speculate this may alter placental development.
Áreas temáticas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: ana.megia@urv.cat
Identificador del autor: 0000-0002-5101-9452
Fecha de alta del registro: 2023-02-19
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/1422-0067/21/2/468
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: International Journal Of Molecular Sciences. 21 (2):
Referencia de l'ítem segons les normes APA: Hoch, Denise; Bachbauer, Martina; Poechlauer, Caroline; Algaba-Chueca, Francisco; Tandl, Veronika; Novakovic, Boris; Megia, Ana; Gauster, Martin; Saff (2020). Maternal Obesity Alters Placental Cell Cycle Regulators in the First Trimester of Human Pregnancy: New Insights for BRCA1. International Journal Of Molecular Sciences, 21(2), -. DOI: 10.3390/ijms21020468
DOI del artículo: 10.3390/ijms21020468
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Tipo de publicación: Journal Publications
Repositori URV