Author, as appears in the article.: Peña, KB; Riu, F; Gumà, J; Guilarte, C; Pique, B; Hernandez, A; Avila, A; Parra, S; Castro, A; Rovira, C; Cueto, P; Vallverdu, I; Parada, D
Department: Medicina i Cirurgia
URV's Author/s: Castro Salomó, Antoni / Gumà Padró, José / Parada Dominguez, David / Parra Pérez, Sandra / Piqué Smith, Berta / Rovira Anglès, Conxita / Vallverdú Perapoch, Inmaculada
Keywords: Thrombocyte Target cell Sars-cov-2 Rna Prospective study Prognosis Plasma Neutrophil Morphology Monocyte Male Lymphocyte Immunohistochemistry Immunohistochemical Human tissue Human cell Human Female Covid-19 testing Clinical article Cell migration Case report Buffy coat Blood Article Adult
Abstract: The pandemic caused by the SARS-CoV-2 infection affects many aspects of public health knowledge, science, and practice around the world. Several studies have shown that SARS-CoV-2 RNA in plasma seems to be associated with a worse prognosis of COVID-19. In the present study, we investigated plasma and buffy RNA in patients with COVID-19 to determine its prognostic value. A prospective study was carried out in patients hospitalized for COVID-19, in which RNA was analyzed in plasma and the buffy coat. Morphological and immunohistochemical studies were used to detect the presence of SARS-CoV-2 in the buffy coat. In COVID-19 patients, the obtained RNA concentration in plasma was 448.3 +/- 31.30 ng/mL. Of all the patients with positive plasma tests for SARS-CoV-2, 46.15% died from COVID-19. In four cases, tests revealed that SARS-CoV-2 was present in the buffy coat. Abnormal morphology of monocytes, lymphocytes and neutrophils was found. An immunohistochemical study showed positivity in mononuclear cells and platelets. Our results suggest that SARS-CoV-2 is present in the plasma. This facilitates viral dissemination and migration to specific organs, where SARS-CoV-2 infects target cells by binding to their receptors. In our study, the presence of plasma SARS-CoV-2 RNA was correlated with worse prognoses.
Thematic Areas: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: conxita.rovira@urv.cat david.parada@urv.cat inmaculada.vallverdu@urv.cat berta.pique@estudiants.urv.cat sandra.parra@urv.cat jose.guma@urv.cat antoni.castro@urv.cat
Author identifier: 0000-0001-9363-6574 0000-0001-7541-9832 0000-0001-5441-6333
Record's date: 2024-07-27
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2076-0817/10/7/805
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Pathogens. 10 (7):
APA: Peña, KB; Riu, F; Gumà, J; Guilarte, C; Pique, B; Hernandez, A; Avila, A; Parra, S; Castro, A; Rovira, C; Cueto, P; Vallverdu, I; Parada, D (2021). Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection-A Preliminary Report. Pathogens, 10(7), -. DOI: 10.3390/pathogens10070805
Article's DOI: 10.3390/pathogens10070805
Entity: Universitat Rovira i Virgili
Journal publication year: 2021
Publication Type: Journal Publications
Repositori URV