Articles producció científica> Medicina i Cirurgia

Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection-A Preliminary Report

  • Datos identificativos

    Identificador: imarina:9225161
    Autores:
    Peña, KBRiu, FGumà, JGuilarte, CPique, BHernandez, AAvila, AParra, SCastro, ARovira, CCueto, PVallverdu, IParada, D
    Resumen:
    The pandemic caused by the SARS-CoV-2 infection affects many aspects of public health knowledge, science, and practice around the world. Several studies have shown that SARS-CoV-2 RNA in plasma seems to be associated with a worse prognosis of COVID-19. In the present study, we investigated plasma and buffy RNA in patients with COVID-19 to determine its prognostic value. A prospective study was carried out in patients hospitalized for COVID-19, in which RNA was analyzed in plasma and the buffy coat. Morphological and immunohistochemical studies were used to detect the presence of SARS-CoV-2 in the buffy coat. In COVID-19 patients, the obtained RNA concentration in plasma was 448.3 +/- 31.30 ng/mL. Of all the patients with positive plasma tests for SARS-CoV-2, 46.15% died from COVID-19. In four cases, tests revealed that SARS-CoV-2 was present in the buffy coat. Abnormal morphology of monocytes, lymphocytes and neutrophils was found. An immunohistochemical study showed positivity in mononuclear cells and platelets. Our results suggest that SARS-CoV-2 is present in the plasma. This facilitates viral dissemination and migration to specific organs, where SARS-CoV-2 infects target cells by binding to their receptors. In our study, the presence of plasma SARS-CoV-2 RNA was correlated with worse prognoses.
  • Otros:

    Autor según el artículo: Peña, KB; Riu, F; Gumà, J; Guilarte, C; Pique, B; Hernandez, A; Avila, A; Parra, S; Castro, A; Rovira, C; Cueto, P; Vallverdu, I; Parada, D
    Departamento: Medicina i Cirurgia
    Autor/es de la URV: Castro Salomó, Antoni / Gumà Padró, José / Parada Dominguez, David / Parra Pérez, Sandra / Piqué Smith, Berta / Rovira Anglès, Conxita / Vallverdú Perapoch, Inmaculada
    Palabras clave: Thrombocyte Target cell Sars-cov-2 Rna Prospective study Prognosis Plasma Neutrophil Morphology Monocyte Male Lymphocyte Immunohistochemistry Immunohistochemical Human tissue Human cell Human Female Covid-19 testing Clinical article Cell migration Case report Buffy coat Blood Article Adult
    Resumen: The pandemic caused by the SARS-CoV-2 infection affects many aspects of public health knowledge, science, and practice around the world. Several studies have shown that SARS-CoV-2 RNA in plasma seems to be associated with a worse prognosis of COVID-19. In the present study, we investigated plasma and buffy RNA in patients with COVID-19 to determine its prognostic value. A prospective study was carried out in patients hospitalized for COVID-19, in which RNA was analyzed in plasma and the buffy coat. Morphological and immunohistochemical studies were used to detect the presence of SARS-CoV-2 in the buffy coat. In COVID-19 patients, the obtained RNA concentration in plasma was 448.3 +/- 31.30 ng/mL. Of all the patients with positive plasma tests for SARS-CoV-2, 46.15% died from COVID-19. In four cases, tests revealed that SARS-CoV-2 was present in the buffy coat. Abnormal morphology of monocytes, lymphocytes and neutrophils was found. An immunohistochemical study showed positivity in mononuclear cells and platelets. Our results suggest that SARS-CoV-2 is present in the plasma. This facilitates viral dissemination and migration to specific organs, where SARS-CoV-2 infects target cells by binding to their receptors. In our study, the presence of plasma SARS-CoV-2 RNA was correlated with worse prognoses.
    Áreas temáticas: Molecular biology Microbiology (medical) Microbiology Infectious diseases Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Immunology and allergy General immunology and microbiology Farmacia
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: conxita.rovira@urv.cat david.parada@urv.cat inmaculada.vallverdu@urv.cat berta.pique@estudiants.urv.cat sandra.parra@urv.cat jose.guma@urv.cat antoni.castro@urv.cat
    Identificador del autor: 0000-0001-9363-6574 0000-0001-7541-9832 0000-0001-5441-6333
    Fecha de alta del registro: 2024-07-27
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2076-0817/10/7/805
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Pathogens. 10 (7):
    Referencia de l'ítem segons les normes APA: Peña, KB; Riu, F; Gumà, J; Guilarte, C; Pique, B; Hernandez, A; Avila, A; Parra, S; Castro, A; Rovira, C; Cueto, P; Vallverdu, I; Parada, D (2021). Study of the Plasma and Buffy Coat in Patients with SARS-CoV-2 Infection-A Preliminary Report. Pathogens, 10(7), -. DOI: 10.3390/pathogens10070805
    DOI del artículo: 10.3390/pathogens10070805
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Immunology and Allergy,Immunology and Microbiology (Miscellaneous),Infectious Diseases,Microbiology,Microbiology (Medical),Molecular Biology
    Thrombocyte
    Target cell
    Sars-cov-2
    Rna
    Prospective study
    Prognosis
    Plasma
    Neutrophil
    Morphology
    Monocyte
    Male
    Lymphocyte
    Immunohistochemistry
    Immunohistochemical
    Human tissue
    Human cell
    Human
    Female
    Covid-19 testing
    Clinical article
    Cell migration
    Case report
    Buffy coat
    Blood
    Article
    Adult
    Molecular biology
    Microbiology (medical)
    Microbiology
    Infectious diseases
    Immunology and microbiology (miscellaneous)
    Immunology and microbiology (all)
    Immunology and allergy
    General immunology and microbiology
    Farmacia
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