Author, as appears in the article.: Urpi-Sarda, Mireia; Casas, Rosa; Sacanella, Emilio; Corella, Dolores; Andres-Lacueva, Cristina; Llorach, Rafael; Garrabou, Gloria; Cardellach, Francesc; Sala-Vila, Aleix; Ros, Emilio; Ruiz-Canela, Miguel; Fito, Montserrat; Salas-Salvado, Jordi; Estruch, Ramon;

Department: Bioquímica i Biotecnologia

URV's Author/s: Salas Salvadó, Jorge / Urpi Sarda, Mireia

Keywords: Style diet Nutrition Nlrp3 inflammasome Markers Low-grade inflammation Inflammation Immunity Gene expression Dysfunction Dietary pattern Cxcl5 Cholesterol Chemokines Cardiovascular diseases Atherosclerosis

Abstract: The intervention with the Mediterranean diet (MD) pattern has evidenced short-term anti-inflammatory effects, but little is known about its long-term anti-inflammatory properties at molecular level. This study aims to investigate the 3-year effect of MD interventions compared to low-fat diet (LFD) on changes on inflammatory biomarkers related to atherosclerosis in a free-living population with a high-risk of cardiovascular disease (CD). Participants (n = 285) in the PREDIMED trial were randomly assigned into three intervention groups: MD with extra-virgin olive oil (EVOO) or MD-Nuts, and a LFD. Fourteen plasma inflammatory biomarkers were determined by Luminex assays. An additional pilot study of gene expression (GE) was determined by RT-PCR in 35 participants. After 3 years, both MDs showed a significant reduction in the plasma levels of IL-1 beta, IL-6, IL-8, TNF-alpha, IFN-gamma, hs-CRP, MCP-1, MIP-1 beta, RANTES, and ENA78 (p < 0.05; all). The decreased levels of IL-1 beta, IL-6, IL-8, and TNF-alpha after MD significantly differed from those in the LFD (p < 0.05). No significant changes were observed at the gene level after MD interventions, however, the GE of CXCR2 and CXCR3 tended to increase in the control LFD group (p = 0.09). This study supports the implementation of MD as a healthy long-term dietary pattern in the prevention of CD in populations at high cardiovascular risk.

Thematic Areas: Pharmacology & pharmacy Medicine, research & experimental Medicine (miscellaneous) General biochemistry,genetics and molecular biology Ciencias sociales Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Biochemistry & molecular biology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: mireia.urpi@urv.cat jordi.salas@urv.cat

Author identifier: 0000-0003-2700-7459

Record's date: 2023-02-26

Journal volume: 9

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://www.mdpi.com/2227-9059/9/8/862

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Biomedicines. 9 (8):

APA: Urpi-Sarda, Mireia; Casas, Rosa; Sacanella, Emilio; Corella, Dolores; Andres-Lacueva, Cristina; Llorach, Rafael; Garrabou, Gloria; Cardellach, Frances (2021). The 3-Year Effect of the Mediterranean Diet Intervention on Inflammatory Biomarkers Related to Cardiovascular Disease. Biomedicines, 9(8), -. DOI: 10.3390/biomedicines9080862

Article's DOI: 10.3390/biomedicines9080862

Entity: Universitat Rovira i Virgili

Journal publication year: 2021

Publication Type: Journal Publications