Articles producció científicaMedicina i Cirurgia

Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme

  • Identification data

    Identifier:  imarina:9267551
    Handle: https://hdl.handle.net/20.500.11797/imarina9267551
    Authors:  Lopez, Maria; Mayer, Celia; Fernandez-Garcia, Laura; Blasco, Lucia; Muras, Andrea; Martin Ruiz, Federico; Bou, German; Otero, Ana; Tomas, Maria
    Abstract:
    Acinetobacter baumannii is an important pathogen that causes nosocomial infections generally associated with high mortality and morbidity in Intensive Care Units (ICUs). Currently, little is known about the Quorum Sensing (QS)/Quorum Quenching (QQ) systems of this pathogen. We analyzed these mechanisms in seven clinical isolates of A. baumannii. Micro array analysis of one of these clinical isolates, Ab1 (A. baumanniiST-2_clon_2010), previously cultured in the presence of 3-oxo-C12-HSL (a QS signalling molecule) revealed a putative QQ enzyme (a/beta hydrolase gene, AidA). This QQ enzyme was present in all non motile clinical isolates (67% of which were isolated from the respiratory tract) cultured in nutrient depleted LB medium. Interestingly, this gene was not located in the genome of the only motile clinical strain growing in this medium (A. baumannii strain Ab421_GEIH-2010 [Ab7], isolated from a blood sample). The AidA protein expressed in E. coli showed QQ activity. Finally, we observed downregulation of the AidA protein (QQ system attenuation) in the presence of H2O2 (ROS stress). In conclusion, most of the A. baumanni clinical strains were not surface motile (84%) and were of respiratory origin (67%). Only the pilT gene was involved in surface motility and related to the QS system. Finally, a new QQ enzyme (a/beta hydrolase gene, AidA protein) was detected in these strains.

  • Others:

    Link to the original source: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174454
    APA: Lopez, Maria; Mayer, Celia; Fernandez-Garcia, Laura; Blasco, Lucia; Muras, Andrea; Martin Ruiz, Federico; Bou, German; Otero, Ana; Tomas, Maria (2017). Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme. Plos One, 12(3), e0174454-. DOI: 10.1371/journal.pone.0174454
    Paper original source: Plos One. 12 (3): e0174454-
    Article's DOI: 10.1371/journal.pone.0174454
    Journal publication year: 2017
    Entity: Universitat Rovira i Virgili
    Paper version: info:eu-repo/semantics/publishedVersion
    Record's date: 2025-02-08
    URV's Author/s: Iftimie Iftimie, Simona Mihaela
    Department: Medicina i Cirurgia
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Publication Type: Journal Publications
    Author, as appears in the article.: Lopez, Maria; Mayer, Celia; Fernandez-Garcia, Laura; Blasco, Lucia; Muras, Andrea; Martin Ruiz, Federico; Bou, German; Otero, Ana; Tomas, Maria
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Thematic Areas: Zootecnia / recursos pesqueiros, Sociology, Sociología, Serviço social, Saúde coletiva, Química, Psychology, Psicología, Planejamento urbano e regional / demografia, Odontología, Nutrição, Multidisciplinary sciences, Multidisciplinary, Medicine (miscellaneous), Medicina veterinaria, Medicina iii, Medicina ii, Medicina i, Materiais, Matemática / probabilidade e estatística, Linguística e literatura, Letras / linguística, Interdisciplinary research in the social sciences, Interdisciplinar, Human geography and urban studies, History & philosophy of science, Historia, Geografía, Geociências, General medicine, General biochemistry,genetics and molecular biology, General agricultural and biological sciences, Farmacia, Environmental studies, Ensino, Engenharias iv, Engenharias iii, Engenharias ii, Engenharias i, Enfermagem, Educação física, Educação, Economia, Direito, Demography, Comunicação e informação, Ciências sociais aplicadas i, Ciências biológicas iii, Ciências biológicas ii, Ciências biológicas i, Ciências ambientais, Ciências agrárias i, Ciência política e relações internacionais, Ciência de alimentos, Ciência da computação, Biotecnología, Biology, Biodiversidade, Biochemistry, genetics and molecular biology (miscellaneous), Astronomia / física, Arquitetura, urbanismo e design, Archaeology, Antropologia / arqueologia, Anthropology, Agricultural and biological sciences (miscellaneous), Administração, ciências contábeis e turismo, Administração pública e de empresas, ciências contábeis e turismo
    Author's mail: simonamihaela.iftime@urv.cat

  • Keywords:

    Signal
    Pseudomonas-aeruginosa pao1
    Oxidative stress
    N-acylhomoserine lactonase
    Efflux pumps
    Biofilm formation
    Autoinducer-2
    Agrobacterium-tumefaciens
    Acyl-homoserine lactone
    Acinetobacter-baumannii
    Agricultural and Biological Sciences (Miscellaneous)
    Biochemistry
    Genetics and Molecular Biology (Miscellaneous)
    Biology
    Medicine (Miscellaneous)
    Multidisciplinary
    Multidisciplinary Sciences
    Zootecnia / recursos pesqueiros
    Sociology
    Sociología
    Serviço social
    Saúde coletiva
    Química
    Psychology
    Psicología
    Planejamento urbano e regional / demografia
    Odontología
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Linguística e literatura
    Letras / linguística
    Interdisciplinary research in the social sciences
    Interdisciplinar
    Human geography and urban studies
    History & philosophy of science
    Historia
    Geografía
    Geociências
    General medicine
    General biochemistry
    genetics and molecular biology
    General agricultural and biological sciences
    Farmacia
    Environmental studies
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Enfermagem
    Educação física
    Educação
    Economia
    Direito
    Demography
    Comunicação e informação
    Ciências sociais aplicadas i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência política e relações internacionais
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
    Astronomia / física
    Arquitetura
    urbanismo e design
    Archaeology
    Antropologia / arqueologia
    Anthropology
    Administração
    ciências contábeis e turismo
    Administração pública

  • Documents:

    DocumentPrincipal

  • Cerca a google

    Search to google scholar