Autor según el artículo: Lopez, Maria; Mayer, Celia; Fernandez-Garcia, Laura; Blasco, Lucia; Muras, Andrea; Martin Ruiz, Federico; Bou, German; Otero, Ana; Tomas, Maria;GEIH-GEMARA SEIMC
Departamento: Medicina i Cirurgia
Autor/es de la URV: Iftimie Iftimie, Simona Mihaela
Palabras clave: Acinetobacter-baumannii Acyl-homoserine lactone Agrobacterium-tumefaciens Autoinducer-2 Biofilm formation Efflux pumps N-acylhomoserine lactonase Oxidative stress Pseudomonas-aeruginosa pao1 Signal
Resumen: Acinetobacter baumannii is an important pathogen that causes nosocomial infections generally associated with high mortality and morbidity in Intensive Care Units (ICUs). Currently, little is known about the Quorum Sensing (QS)/Quorum Quenching (QQ) systems of this pathogen. We analyzed these mechanisms in seven clinical isolates of A. baumannii. Micro array analysis of one of these clinical isolates, Ab1 (A. baumanniiST-2_clon_2010), previously cultured in the presence of 3-oxo-C12-HSL (a QS signalling molecule) revealed a putative QQ enzyme (a/beta hydrolase gene, AidA). This QQ enzyme was present in all non motile clinical isolates (67% of which were isolated from the respiratory tract) cultured in nutrient depleted LB medium. Interestingly, this gene was not located in the genome of the only motile clinical strain growing in this medium (A. baumannii strain Ab421_GEIH-2010 [Ab7], isolated from a blood sample). The AidA protein expressed in E. coli showed QQ activity. Finally, we observed downregulation of the AidA protein (QQ system attenuation) in the presence of H2O2 (ROS stress). In conclusion, most of the A. baumanni clinical strains were not surface motile (84%) and were of respiratory origin (67%). Only the pilT gene was involved in surface motility and related to the QS system. Finally, a new QQ enzyme (a/beta hydrolase gene, AidA protein) was detected in these strains.
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Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: simonamihaela.iftime@urv.cat simonamihaela.iftime@urv.cat
Identificador del autor: 0000-0003-0714-8414 0000-0003-0714-8414
Fecha de alta del registro: 2022-07-16
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174454
Referencia al articulo segun fuente origial: Plos One. 12 (3): e0174454-
Referencia de l'ítem segons les normes APA: Lopez, Maria; Mayer, Celia; Fernandez-Garcia, Laura; Blasco, Lucia; Muras, Andrea; Martin Ruiz, Federico; Bou, German; Otero, Ana; Tomas, Maria;GEIH-G (2017). Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme. Plos One, 12(3), e0174454-. DOI: 10.1371/journal.pone.0174454
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1371/journal.pone.0174454
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2017
Tipo de publicación: Journal Publications
Repositori URV