Repositori URV

Autor según el artículo: Lopez, Maria; Mayer, Celia; Fernandez-Garcia, Laura; Blasco, Lucia; Muras, Andrea; Martin Ruiz, Federico; Bou, German; Otero, Ana; Tomas, Maria;GEIH-GEMARA SEIMC

Departamento: Medicina i Cirurgia

Autor/es de la URV: Iftimie Iftimie, Simona Mihaela

Palabras clave: Signal Pseudomonas-aeruginosa pao1 Oxidative stress N-acylhomoserine lactonase Efflux pumps Biofilm formation Autoinducer-2 Agrobacterium-tumefaciens Acyl-homoserine lactone Acinetobacter-baumannii

Resumen: Acinetobacter baumannii is an important pathogen that causes nosocomial infections generally associated with high mortality and morbidity in Intensive Care Units (ICUs). Currently, little is known about the Quorum Sensing (QS)/Quorum Quenching (QQ) systems of this pathogen. We analyzed these mechanisms in seven clinical isolates of A. baumannii. Micro array analysis of one of these clinical isolates, Ab1 (A. baumanniiST-2_clon_2010), previously cultured in the presence of 3-oxo-C12-HSL (a QS signalling molecule) revealed a putative QQ enzyme (a/beta hydrolase gene, AidA). This QQ enzyme was present in all non motile clinical isolates (67% of which were isolated from the respiratory tract) cultured in nutrient depleted LB medium. Interestingly, this gene was not located in the genome of the only motile clinical strain growing in this medium (A. baumannii strain Ab421_GEIH-2010 [Ab7], isolated from a blood sample). The AidA protein expressed in E. coli showed QQ activity. Finally, we observed downregulation of the AidA protein (QQ system attenuation) in the presence of H2O2 (ROS stress). In conclusion, most of the A. baumanni clinical strains were not surface motile (84%) and were of respiratory origin (67%). Only the pilT gene was involved in surface motility and related to the QS system. Finally, a new QQ enzyme (a/beta hydrolase gene, AidA protein) was detected in these strains.

Áreas temáticas: Zootecnia / recursos pesqueiros Sociology Sociología Serviço social Saúde coletiva Química Psychology Psicología Planejamento urbano e regional / demografia Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Linguística e literatura Letras / linguística Interdisciplinary research in the social sciences Interdisciplinar Human geography and urban studies History & philosophy of science Historia Geografía Geociências General medicine General biochemistry,genetics and molecular biology General agricultural and biological sciences Farmacia Environmental studies Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Enfermagem Educação física Educação Economia Direito Demography Comunicação e informação Ciências sociais aplicadas i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência política e relações internacionais Ciência de alimentos Ciência da computação Biotecnología Biology Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Astronomia / física Arquitetura, urbanismo e design Archaeology Antropologia / arqueologia Anthropology Agricultural and biological sciences (miscellaneous) Administração, ciências contábeis e turismo Administração pública e de empresas, ciências contábeis e turismo

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: simonamihaela.iftime@urv.cat

Identificador del autor: 0000-0003-0714-8414

Fecha de alta del registro: 2024-09-07

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174454

URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Plos One. 12 (3): e0174454-

Referencia de l'ítem segons les normes APA: Lopez, Maria; Mayer, Celia; Fernandez-Garcia, Laura; Blasco, Lucia; Muras, Andrea; Martin Ruiz, Federico; Bou, German; Otero, Ana; Tomas, Maria;GEIH-G (2017). Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme. Plos One, 12(3), e0174454-. DOI: 10.1371/journal.pone.0174454

DOI del artículo: 10.1371/journal.pone.0174454

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2017

Tipo de publicación: Journal Publications