Articles producció científica> Enginyeria Química

Cyclodextrin polymer clean-up method for the detection of ciguatoxins in fish with cell-based assays

  • Identification data

    Identifier: imarina:9282207
  • Authors:

    Campàs, M
    Leonardo, S
    Rambla-Alegre, M
    Sagristà, N
    Vaya, R
    Diogène, J
    Torréns, M
    Fragoso, A
  • Others:

    Author, as appears in the article.: Campàs, M; Leonardo, S; Rambla-Alegre, M; Sagristà, N; Vaya, R; Diogène, J; Torréns, M; Fragoso, A
    Department: Enginyeria Química
    URV's Author/s: CAMPAS HOMS, MONICA / Fragoso Sierra, Alex
    Keywords: Variola louti Seriola dumerili Matrix effects Cyclodextrin (cd) polymer Ciguatoxin (ctx) Ciguatera Cell-based assay (cba) variola louti seriola dumerili samples matrix effects ciguatoxin (ctx) cell -based assay (cba) brevetoxins bioassay
    Abstract: Ciguatoxins (CTXs) are marine toxins produced by microalgae of the genera Gambierdiscus and Fukuyoa, which are transferred through the food webs, reaching humans and causing a poisoning known as ciguatera. The cell-based assay (CBA) is commonly used for their detection because of its high sensitivity and the provided toxicological information. However, matrix effects may interfere in the CBA. In this work, γ-cyclodextrin-hexamethylene diisocyanate (γ-CD-HDI), γ-cyclodextrin-epichlorohydrin (γ-CD-EPI) and γ-CD-EPI conjugated to magnetic beads (γ-CD-EPI-MB) have been evaluated as clean-up materials for fish flesh extracts containing CTXs. The best results were achieved with γ-CD-HDI in column format, which showed a CTX1B recovery of 42% and 32% for Variola louti and Seriola dumerili, respectively, and allowed exposing cells to at least 400 mg/mL of fish flesh. This clean-up strategy provides at least 4.6 and 3.0-fold higher sensitivities to the assay for V. louti and S. dumerili, respectively, improving the reliability of CTX quantification.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geociências Food science & technology Food science Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Biotecnología Biodiversidade Astronomia / física Analytical chemistry Administração pública e de empresas, ciências contábeis e turismo
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: monica.campas@urv.cat alex.fragoso@urv.cat
    Author identifier: 0000-0002-1220-7100 0000-0003-4839-6094
    Record's date: 2023-12-16
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0308814622021586
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Food Chemistry. 401 134196-
    APA: Campàs, M; Leonardo, S; Rambla-Alegre, M; Sagristà, N; Vaya, R; Diogène, J; Torréns, M; Fragoso, A (2023). Cyclodextrin polymer clean-up method for the detection of ciguatoxins in fish with cell-based assays. Food Chemistry, 401(), 134196-. DOI: 10.1016/j.foodchem.2022.134196
    Article's DOI: 10.1016/j.foodchem.2022.134196
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Chemistry, Applied,Food Science,Food Science & Technology,Medicine (Miscellaneous),Nutrition & Dietetics
    Variola louti
    Seriola dumerili
    Matrix effects
    Cyclodextrin (cd) polymer
    Ciguatoxin (ctx)
    Ciguatera
    Cell-based assay (cba)
    variola louti
    seriola dumerili
    samples
    matrix effects
    ciguatoxin (ctx)
    cell -based assay (cba)
    brevetoxins
    bioassay
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrition & dietetics
    Nutrição
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Geociências
    Food science & technology
    Food science
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Enfermagem
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, applied
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
    Administração pública e de empresas, ciências contábeis e turismo
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