Author, as appears in the article.: Campas, Monica; Leonardo, Sandra; Rambla-Alegre, Maria; Sagrista, Nuria; Vaya, Raquel; Diogene, Jorge; Torrens, Mabel; Fragoso, Alex
Department: Enginyeria Química
URV's Author/s: CAMPAS HOMS, MONICA / Fragoso Sierra, Alex / TORRENS DEL VALLE, MABEL
Keywords: Variola louti; Seriola dumerili; Reproducibility of results; Matrix effects; Marine toxins; Humans; Gamma-cyclodextrins; Fishes; Epichlorohydrin; Dinoflagellida; Cyclodextrin polymer; Cyclodextrin (cd) polymer; Ciguatoxins; Ciguatoxin (ctx); Ciguatera; Cell-based assay (cba); Animals; variola louti; seriola dumerili; samples; matrix effects; ciguatoxin (ctx); cell -based assay (cba); brevetoxins; bioassay
Abstract: Ciguatoxins (CTXs) are marine toxins produced by microalgae of the genera Gambierdiscus and Fukuyoa, which are transferred through the food webs, reaching humans and causing a poisoning known as ciguatera. The cell-based assay (CBA) is commonly used for their detection because of its high sensitivity and the provided toxicological information. However, matrix effects may interfere in the CBA. In this work, γ-cyclodextrin-hexamethylene diisocyanate (γ-CD-HDI), γ-cyclodextrin-epichlorohydrin (γ-CD-EPI) and γ-CD-EPI conjugated to magnetic beads (γ-CD-EPI-MB) have been evaluated as clean-up materials for fish flesh extracts containing CTXs. The best results were achieved with γ-CD-HDI in column format, which showed a CTX1B recovery of 42% and 32% for Variola louti and Seriola dumerili, respectively, and allowed exposing cells to at least 400 mg/mL of fish flesh. This clean-up strategy provides at least 4.6 and 3.0-fold higher sensitivities to the assay for V. louti and S. dumerili, respectively, improving the reliability of CTX quantification.
Thematic Areas: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Odontología; Nutrition & dietetics; Nutrição; Medicine (miscellaneous); Medicina veterinaria; Medicina ii; Medicina i; Materiais; Matemática / probabilidade e estatística; Interdisciplinar; Geociências; Food science & technology; Food science; Farmacia; Ensino; Engenharias iv; Engenharias iii; Engenharias ii; Engenharias i; Enfermagem; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Ciência da computação; Chemistry, applied; Biotecnología; Biodiversidade; Astronomia / física; Analytical chemistry; Administração pública e de empresas, ciências contábeis e turismo
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: monica.campas@urv.cat; alex.fragoso@urv.cat
Record's date: 2025-02-18
Paper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0308814622021586
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Food Chemistry. 401 134196-
APA: Campas, Monica; Leonardo, Sandra; Rambla-Alegre, Maria; Sagrista, Nuria; Vaya, Raquel; Diogene, Jorge; Torrens, Mabel; Fragoso, Alex (2023). Cyclodextrin polymer clean-up method for the detection of ciguatoxins in fish with cell-based assays. Food Chemistry, 401(), 134196-. DOI: 10.1016/j.foodchem.2022.134196
Article's DOI: 10.1016/j.foodchem.2022.134196
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV