Author, as appears in the article.: Campas, Monica; Leonardo, Sandra; Rambla-Alegre, Maria; Sagrista, Nuria; Vaya, Raquel; Diogene, Jorge; Torrens, Mabel; Fragoso, Alex
Department: Enginyeria Química
URV's Author/s: CAMPAS HOMS, MONICA / Fragoso Sierra, Alex / TORRENS DEL VALLE, MABEL
Keywords: Variola louti Seriola dumerili Matrix effects Cyclodextrin (cd) polymer Ciguatoxin (ctx) Ciguatera Cell-based assay (cba) variola louti seriola dumerili samples matrix effects ciguatoxin (ctx) cell -based assay (cba) brevetoxins bioassay
Abstract: Ciguatoxins (CTXs) are marine toxins produced by microalgae of the genera Gambierdiscus and Fukuyoa, which are transferred through the food webs, reaching humans and causing a poisoning known as ciguatera. The cell-based assay (CBA) is commonly used for their detection because of its high sensitivity and the provided toxicological information. However, matrix effects may interfere in the CBA. In this work, γ-cyclodextrin-hexamethylene diisocyanate (γ-CD-HDI), γ-cyclodextrin-epichlorohydrin (γ-CD-EPI) and γ-CD-EPI conjugated to magnetic beads (γ-CD-EPI-MB) have been evaluated as clean-up materials for fish flesh extracts containing CTXs. The best results were achieved with γ-CD-HDI in column format, which showed a CTX1B recovery of 42% and 32% for Variola louti and Seriola dumerili, respectively, and allowed exposing cells to at least 400 mg/mL of fish flesh. This clean-up strategy provides at least 4.6 and 3.0-fold higher sensitivities to the assay for V. louti and S. dumerili, respectively, improving the reliability of CTX quantification.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geociências Food science & technology Food science Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Engenharias i Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Biotecnología Biodiversidade Astronomia / física Analytical chemistry Administração pública e de empresas, ciências contábeis e turismo
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: monica.campas@urv.cat alex.fragoso@urv.cat
Author identifier: 0000-0002-1220-7100 0000-0003-4839-6094
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Food Chemistry. 401 134196-
APA: Campas, Monica; Leonardo, Sandra; Rambla-Alegre, Maria; Sagrista, Nuria; Vaya, Raquel; Diogene, Jorge; Torrens, Mabel; Fragoso, Alex (2023). Cyclodextrin polymer clean-up method for the detection of ciguatoxins in fish with cell-based assays. Food Chemistry, 401(), 134196-. DOI: 10.1016/j.foodchem.2022.134196
Entity: Universitat Rovira i Virgili
Journal publication year: 2023
Publication Type: Journal Publications
Repositori URV