Articles producció científica> Medicina i Cirurgia

Increased Hepatic ATG7 mRNA and ATG7 Protein Expression in Nonalcoholic Steatohepatitis Associated with Obesity

  • Identification data

    Identifier: imarina:9291523
    Authors:
    Barrientos-Riosalido, AndreaReal, MonicaBertran, LaiaAguilar, CarmenMartinez, SalomeParada, DavidVives, MargaritaSabench, FatimaRiesco, DavidCastillo, Daniel DelRichart, CristobalAuguet, Teresa
    Abstract:
    The autophagy gene ATG7 has been shown to be essential for the induction of autophagy, a process that used to be suppressed in nonalcoholic fatty liver disease (NAFLD). However, the specific role of ATG7 in NAFLD remains unclear. The aim of this study was to analyze hepatic ATG7 mRNA and ATG7 protein expression regarding obesity-associated NAFLD. Patients included women classified into normal weight (NW, n = 6) and morbid obesity (MO, n = 72). The second group was subclassified into normal liver (NL, n = 11), simple steatosis (SS, n= 29), and nonalcoholic steatohepatitis (NASH, n = 32). mRNA expression was analyzed by RT–qPCR and protein expression was evaluated by Western blotting. Our results showed that NASH patients presented higher ATG7 mRNA and ATG7 protein levels. ATG7 mRNA expression was increased in NASH compared with SS, while ATG7 protein abundance was enhanced in NASH compared with NL. ATG7 mRNA correlated negatively with the expression of some hepatic lipid metabolism-related genes and positively with endocannabinoid receptors, adiponectin hepatic expression, and omentin levels. These results suggest that ATG7-mediated autophagy may play an important role in the pathogenesis of NAFLD, especially in NASH, perhaps playing a possible protective role. However, this is a preliminary study that needs to be further studied.
  • Others:

    Author, as appears in the article.: Barrientos-Riosalido, Andrea; Real, Monica; Bertran, Laia; Aguilar, Carmen; Martinez, Salome; Parada, David; Vives, Margarita; Sabench, Fatima; Riesco, David; Castillo, Daniel Del; Richart, Cristobal; Auguet, Teresa
    Department: Medicina i Cirurgia
    URV's Author/s: Aguilar Crespillo, Carmen Isabel / Auguet Quintillà, Maria Teresa / Barrientos Riosalido, Andrea / Bertran Ramos, Laia / Del Castillo Déjardin, Daniel / Martínez González, María Salomé / Richart Jurado, Cristobal Manuel / Sabench Pereferrer, Fàtima / Vives Espelta, Margarida
    Keywords: Rna, messenger Obesity Nonalcoholic steatohepatitis Non-alcoholic fatty liver disease Nafld Liver Lipid metabolism Humans Female Autophagy-related protein 7 Autophagy Atg7 protein, human Atg7
    Abstract: The autophagy gene ATG7 has been shown to be essential for the induction of autophagy, a process that used to be suppressed in nonalcoholic fatty liver disease (NAFLD). However, the specific role of ATG7 in NAFLD remains unclear. The aim of this study was to analyze hepatic ATG7 mRNA and ATG7 protein expression regarding obesity-associated NAFLD. Patients included women classified into normal weight (NW, n = 6) and morbid obesity (MO, n = 72). The second group was subclassified into normal liver (NL, n = 11), simple steatosis (SS, n= 29), and nonalcoholic steatohepatitis (NASH, n = 32). mRNA expression was analyzed by RT–qPCR and protein expression was evaluated by Western blotting. Our results showed that NASH patients presented higher ATG7 mRNA and ATG7 protein levels. ATG7 mRNA expression was increased in NASH compared with SS, while ATG7 protein abundance was enhanced in NASH compared with NL. ATG7 mRNA correlated negatively with the expression of some hepatic lipid metabolism-related genes and positively with endocannabinoid receptors, adiponectin hepatic expression, and omentin levels. These results suggest that ATG7-mediated autophagy may play an important role in the pathogenesis of NAFLD, especially in NASH, perhaps playing a possible protective role. However, this is a preliminary study that needs to be further studied.
    Thematic Areas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: andrea.barrientos@urv.cat andrea.barrientos@urv.cat margarida.vives@urv.cat mariasalome.martinez@urv.cat danieldel.castillo@urv.cat laia.bertranr@estudiants.urv.cat laia.bertranr@estudiants.urv.cat fatima.sabench@urv.cat carmenisabel.aguilar@urv.cat carmenisabel.aguilar@urv.cat mariateresa.auguet@urv.cat
    Author identifier: 0000-0001-6185-2889 0000-0003-0456-3102 0000-0001-7024-7824 0000-0001-9052-1368 0000-0001-9052-1368 0000-0002-9262-8756 0000-0002-4440-562X 0000-0002-4440-562X 0000-0003-0396-6428
    Record's date: 2024-09-28
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1422-0067/24/2/1324
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: International Journal Of Molecular Sciences. 24 (2): 1324-
    APA: Barrientos-Riosalido, Andrea; Real, Monica; Bertran, Laia; Aguilar, Carmen; Martinez, Salome; Parada, David; Vives, Margarita; Sabench, Fatima; Riesco (2023). Increased Hepatic ATG7 mRNA and ATG7 Protein Expression in Nonalcoholic Steatohepatitis Associated with Obesity. International Journal Of Molecular Sciences, 24(2), 1324-. DOI: 10.3390/ijms24021324
    Article's DOI: 10.3390/ijms24021324
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2023
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Rna, messenger
    Obesity
    Nonalcoholic steatohepatitis
    Non-alcoholic fatty liver disease
    Nafld
    Liver
    Lipid metabolism
    Humans
    Female
    Autophagy-related protein 7
    Autophagy
    Atg7 protein, human
    Atg7
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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