Autor según el artículo: Nicolas Rozès Ricardo Cordero-Otero Montse Poblet Gema López-Martínez Anna Borrull

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: ROZÈS ., NICOLAS ANDRE LOUIS CORDERO OTERO, RICARDO ROMÁN POBLET ICART, MARIA MONTSERRAT LÓPEZ MARTÍNEZ, GEMA ISABEL BORRULL RIERA, ANNA

Palabras clave: medium chain fatty acid decanoic acid derivative Alcohol

Resumen: Octanoic (C8) and decanoic (C10) acids are produced in hypoxic conditions by the yeast Saccharomyces cerevisiae as by-products of its metabolism and are considered fermentation inhibitors in the presence of ethanol at acidic pH. This study aims to broaden our understanding of the physiological limits between toxicity and ester production in yeast cells. To this end, the non-inhibitory concentration (NIC) and maximum inhibitory concentration (MIC) values were first established for C8 and C10 at physiological pH (5.8) without ethanol. The results showed that when these acids were added to culture medium at these values, they tended to accumulate in different cellular fractions of the yeast. While C8 was almost entirely located in the cell wall fraction, C10 was found in the endocellular fraction. Cell fatty acid detoxification was also different; while the esterification of fatty acids was more efficient in the case of C10, the peroxisome was activated regardless of which fatty acid was added. Furthermore, the study of the Pdr12 and Tpo1 transporters that evolved during the detoxification process revealed that C8 was mostly expelled by the Pdr12 carrier, which was related to higher β-oxidative damage in the presence of endocellular C10. C10 is more toxic at lower concentrations than C8. Although they are produced by yeast, the resulting intracellular medium-chain fatty acids (MCFAs) caused a level of toxicity which promoted cell death. However, MCFAs are involved in the production of beverage flavours. © 2015 John Wiley & Sons, Ltd.

Grupo de investigación: Biotecnologia Enològica Biotecnologia Microbiana dels Aliments

Áreas temáticas: Biochemistry and technology Bioquímica y tecnología Bioquímica i biotecnologia

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1097-0061

Identificador del autor: 0000-0001-9718-3429 0000-0001-5402-1278 0000-0002-9958-5736 n/a n/a

Fecha de alta del registro: 2015-08-05

Página final: 460

Volumen de revista: 32

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: http://onlinelibrary.wiley.com/doi/10.1002/yea.3071/full

DOI del artículo: 10.1002/yea.3071

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2015

Página inicial: 451

Tipo de publicación: Article Artículo Article