Autor según el artículo: Gemma Aragonès; Teresa Auguet; Alba Berlanga; Esther Guiu-Jurado; Salomé Martinez; Sandra Armengol; Fàtima Sabench; Rosa Ras; Mercè Hernandez; Carmen Aguilar; Josep Colom; Joan Josep Sirvent; Daniel Del Castillo; Cristóbal Richart

Departamento: Ciències Mèdiques Bàsiques Medicina i Cirurgia Bioquímica i Biotecnologia

Autor/es de la URV: ARAGONÈS BARGALLÓ, GEMMA; AUGUET QUINTILLÀ, MARIA TERESA; BERLANGA BUSTOS, ALBA; GUIU JURADO, ESTHER; MARTÍNEZ GONZÁLEZ, MARÍA SALOMÉ; ARMENGOL LÓPEZ, SANDRA; SABENCH PEREFERRER, FÀTIMA; RAS MALLORQUÍ, MARIA ROSA; Mercè Hernandez; AGUILAR MARMOL, CARME; Josep Colom; SIRVENT CALVERA, JUAN JOSÉ; DEL CASTILLO DÉJARDIN, DANIEL; RICHART JURADO, CRISTOBAL MANUEL

Palabras clave: Està en blanc

Resumen: Background: Non-alcoholic fatty liver disease (NAFLD) causes a wide spectrum of liver damage, ranging from simple steatosis to cirrhosis. However, simple steatosis (SS) and steatohepatitis (NASH) cannot yet be distinguished by clinical or laboratory features. The aim of this study was to assess the relationship between alpha-ketoglutarate and the degrees of NAFLD in morbidly obese patients. Materials and Methods: We used a gas chromatography-quadruple time-of-flight-mass spectrometry analysis to quantify alpha-ketoglutarate in serum from normal-weight subjects (n = 30) and morbidly obese women (n = 97) with or without NAFLD. Results: We found that serum levels of alpha-ketoglutarate were significantly higher in morbidly obese women than in normal-weight women. We showed that circulating levels of alpha-ketoglutarate were lower in lean controls and morbidly obese patients without NAFLD. We also found that alpha-ketoglutarate serum levels were higher in both SS and NASH than in normal liver of morbidly obese patients. However, there was no difference between SS and NASH. Moreover, we observed that circulating levels of alpha-ketoglutarate were associated with glucose metabolism parameters, lipid profile, hepatic enzymes and steatosis degree. In addition, diagnostic performance of alpha-ketoglutarate has been analyzed in NAFLD patients. The AUROC curves from patients with liver steatosis exhibited an acceptable clinical utility. Finally, we showed that the combination of biomarkers (AST, ALT and alpha-ketoglutarate) had the highest accuracy in diagnosing liver steatosis. Conclusion: These findings suggest that alpha-ketoglutarate can determine the presence of non-alcoholic fatty liver in morbidly obese patients but it is not valid a biomarker for NASH.

Grupo de investigación: Grup de Recerca en Medicina Aplicada Hospital Joan XXIII Grup de Recerca Biomèdica HJ23 Unitat de Recerca en Cirurgia

Áreas temáticas: Biochemistry and technology Bioquímica y tecnología Bioquímica i biotecnologia

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1932-6203

Identificador del autor: 0000-0002-1924-9231; 0000-0003-0396-6428; 0000-0002-8650-241X; 0000-0003-0371-7210; n/a; n/a; n/a; n/a; n/a; n/a; n/a; n/a; n/a; n/a

Fecha de alta del registro: 2016-05-18

Volumen de revista: 11

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154601

DOI del artículo: 10.1371/journal.pone.0154601

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2016

Página inicial: Article number e0154601

Tipo de publicación: Article Artículo Article