Autor según el artículo: García-Fuentes E; Santiago-Fernández C; Gutiérrez-Repiso C; Mayas M; Oliva-Olivera W; Coín-Aragüez L; Alcaide J; Ocaña-Wilhelmi L; Vendrell J; Tinahones F; Garrido-Sánchez L
Departamento: Medicina i Cirurgia
Autor/es de la URV: Vendrell Ortega, Juan José
Palabras clave: Suppression Obesity Morbid obesity Mechanisms Insulin-resistance Insulin resistance Hypoxia Hif-1aα Hif-1a? Hif-1 alpha Dysregulation Disease Blood-flow Binding Atp citrate-lyase Adipose tissue Adipocytes
Resumen: Background: A key role for HIF-1 alpha in the promotion and maintenance of dietary obesity has been proposed. We analyzed the association between hypoxia and de novo lipogenesis in human adipose tissue.
Methods: We studied HIF-1 alpha mRNA and protein expression in fasting status in visceral adipose tissue (VAT) from non-obese and morbidly obese subjects, and in VAT from wild-type and ob/ob C57BL6J mice in both fasting and feeding status. We also analyzed the effect of hypoxia on the VAT mRNA expression of genes involved in lipogenesis.
Results: HIF-1 alpha was increased in VAT from morbidly obese subjects. In fasting status, C57BL6J ob/ob mice had a higher VAT HIF-1 alpha mRNA expression than C57BL6J wild-type mice. In feeding status, VAT HIF-1 alpha mRNA expression significantly increased in C57BL6J wild-type, but not in C57BL6J ob/ob mice. In humans, HIF-1 alpha mRNA expression correlated positively with body mass index and insulin resistance. VAT HIF-1 alpha mRNA expression correlated negatively with ACC1, PDHB and SIRT3 mRNA expression, and positively with PPAR-gamma. VAT explants incubated in hypoxia showed reduced SIRT3 and increased PPAR-gamma, SREBP-1c, ACLY, ACC1 and FASN mRNA expression.
Conclusions: Morbidly obese subjects have a higher level of VAT HIF-1 alpha. Postprandial status is associated with an increase in HIF-1 alpha mRNA expression in C57BL6J wild-type mice. Hypoxia alters the mRNA expression of genes involved in de novo lipogenesis in human VAT.
Áreas temáticas: Odontología Nutrição Medicine, research & experimental Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar General medicine General biochemistry,genetics and molecular biology Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all)
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: juanjose.vendrell@urv.cat
Identificador del autor: 0000-0002-6994-6115
Fecha de alta del registro: 2024-09-07
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-015-0732-5
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Journal Of Translational Medicine. 13 (1):
Referencia de l'ítem segons les normes APA: García-Fuentes E; Santiago-Fernández C; Gutiérrez-Repiso C; Mayas M; Oliva-Olivera W; Coín-Aragüez L; Alcaide J; Ocaña-Wilhelmi L; Vendrell J; Tinahon (2015). Hypoxia is associated with a lower expression of genes involved in lipogenesis in visceral adipose tissue. Journal Of Translational Medicine, 13(1), -. DOI: 10.1186/s12967-015-0732-5
DOI del artículo: 10.1186/s12967-015-0732-5
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2015
Tipo de publicación: Journal Publications