Autor según el artículo: Romero A., Jordá T., Rozès N., Martínez-Pastor M., Puig S.
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Rozès, Nicolas Andre Louis
Palabras clave: Yeast Saccharomyces cerevisiae Ole1 Mga2 Iron deficiency Hypoxia Fatty acids Cold saccharomyces cerevisiae ole1 mga2 iron deficiency hypoxia fatty acids cold
Resumen: Unsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerevisiae is Ole1. OLE1 expression is tightly regulated to adapt UFA biosynthesis and lipid bilayer properties to changes in temperature, and in UFA or oxygen availability. Despite iron deficiency being the most extended nutritional disorder worldwide, very little is known about the mechanisms and the biological relevance of fatty acid desaturases regulation in response to iron starvation. In this report, we show that endoplasmic reticulum-anchored transcription factor Mga2 activates OLE1 transcription in response to nutritional and genetic iron deficiencies. Cells lacking MGA2 display low UFA levels and do not grow under iron-limited conditions, unless UFAs are supplemented or OLE1 is overexpressed. The proteasome, E3 ubiquitin ligase Rsp5 and the Cdc48Npl4/Ufd1 complex are required for OLE1 activation during iron depletion. Interestingly, Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. These results reveal the mechanism of OLE1 regulation when iron is scarce and identify the MGA2 auto-regulation as a potential activation strategy in multiple stresses
Áreas temáticas: Química Molecular biology Medicina ii Medicina i Linguística e literatura Interdisciplinar Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Biophysics Biodiversidade Biochemistry & molecular biology
ISSN: 13881981
Direcció de correo del autor: nicolasrozes@urv.cat
Identificador del autor: 0000-0001-9718-3429
Página final: 668
Fecha de alta del registro: 2024-09-07
Volumen de revista: 1863
Versión del articulo depositado: info:eu-repo/semantics/submittedVersion
Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S1388198118300490
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids. 1863 (6): 657-668
Referencia de l'ítem segons les normes APA: Romero A., Jordá T., Rozès N., Martínez-Pastor M., Puig S. (2018). Regulation of yeast fatty acid desaturase in response to iron deficiency. Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids, 1863(6), 657-668. DOI: 10.1016/j.bbalip.2018.03.008
DOI del artículo: 10.1016/j.bbalip.2018.03.008
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2018
Página inicial: 657
Tipo de publicación: Journal Publications