Articles producció científica> Enginyeria Química

Differential protein expression of hippocampal cells associated with heavy metals (Pb, As, and MeHg) neurotoxicity: Deepening into the molecular mechanism of neurodegenerative diseases

  • Datos identificativos

    Identificador: imarina:5132977
    Autores:
    Karri, VenkatanaiduRamos, DavidBauza Martinez, JuliaOdena, AntoniaOliveira, EliandreCoort, Susan L.Evelo, Chris T.Mariman, Edwin C. M.Schuhmacher, MartaKumar, Vikas
    Resumen:
    Chronic exposure to heavy metals such as Pb, As, and MeHg can be associated with an increased risk of developing neurodegenerative diseases. Our in vitro bioassays results showed the potency of heavy metals in the order of Pb < As < MeHg on hippocampal cells. The main objective of this study was combining in vitro label free proteomics and systems biology approach for elucidating patterns of biological response, discovering underlying mechanisms of Pb, As, and MeHg toxicity in hippocampal cells. The omics data was refined by using different filters and normalization and multilevel analysis tools were employed to explore the data visualization. The functional and pathway visualization was performed by using Gene ontology and PathVisio tools. Using these all integrated approaches, we identified significant proteins across treatments within the mitochondrial dysfunction, oxidative stress, ubiquitin proteome dysfunction, and mRNA splicing related to neurodegenerative diseases. The systems biology analysis revealed significant alterations in proteins implicated in Parkinson's disease (PD) and Alzheimer's disease (AD). The current proteomics analysis of three metals support the insight into the proteins involved in neurodegeneration and the altered proteins can be useful for metal-specific biomarkers of exposure and its adverse effects.
  • Otros:

    Autor según el artículo: Karri, Venkatanaidu; Ramos, David; Bauza Martinez, Julia; Odena, Antonia; Oliveira, Eliandre; Coort, Susan L.; Evelo, Chris T.; Mariman, Edwin C. M.; Schuhmacher, Marta; Kumar, Vikas;
    Departamento: Enginyeria Química
    Autor/es de la URV: Kumar, Vikas / Schuhmacher Ansuategui, Marta
    Palabras clave: Proteomics Pathway visualization Neurodegenerative diseases Metal exposure pathway visualization neurodegenerative diseases metal exposure
    Resumen: Chronic exposure to heavy metals such as Pb, As, and MeHg can be associated with an increased risk of developing neurodegenerative diseases. Our in vitro bioassays results showed the potency of heavy metals in the order of Pb < As < MeHg on hippocampal cells. The main objective of this study was combining in vitro label free proteomics and systems biology approach for elucidating patterns of biological response, discovering underlying mechanisms of Pb, As, and MeHg toxicity in hippocampal cells. The omics data was refined by using different filters and normalization and multilevel analysis tools were employed to explore the data visualization. The functional and pathway visualization was performed by using Gene ontology and PathVisio tools. Using these all integrated approaches, we identified significant proteins across treatments within the mitochondrial dysfunction, oxidative stress, ubiquitin proteome dysfunction, and mRNA splicing related to neurodegenerative diseases. The systems biology analysis revealed significant alterations in proteins implicated in Parkinson's disease (PD) and Alzheimer's disease (AD). The current proteomics analysis of three metals support the insight into the proteins involved in neurodegeneration and the altered proteins can be useful for metal-specific biomarkers of exposure and its adverse effects.
    Áreas temáticas: Zootecnia / recursos pesqueiros Química Psicología Odontología Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Ensino Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biophysics Biodiversidade Biochemistry Biochemical research methods Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: vikas.kumar@urv.cat marta.schuhmacher@urv.cat
    Identificador del autor: 0000-0002-9795-5967 0000-0003-4381-2490
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    Enlace a la fuente original: https://www.sciencedirect.com/science/article/abs/pii/S1874391918302641
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Journal Of Proteomics. 187 106-125
    Referencia de l'ítem segons les normes APA: Karri, Venkatanaidu; Ramos, David; Bauza Martinez, Julia; Odena, Antonia; Oliveira, Eliandre; Coort, Susan L.; Evelo, Chris T.; Mariman, Edwin C. M.; (2018). Differential protein expression of hippocampal cells associated with heavy metals (Pb, As, and MeHg) neurotoxicity: Deepening into the molecular mechanism of neurodegenerative diseases. Journal Of Proteomics, 187(), 106-125. DOI: 10.1016/j.jprot.2018.06.020
    DOI del artículo: 10.1016/j.jprot.2018.06.020
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2018
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemical Research Methods,Biochemistry,Biophysics
    Proteomics
    Pathway visualization
    Neurodegenerative diseases
    Metal exposure
    pathway visualization
    neurodegenerative diseases
    metal exposure
    Zootecnia / recursos pesqueiros
    Química
    Psicología
    Odontología
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Farmacia
    Ensino
    Engenharias iii
    Engenharias ii
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biophysics
    Biodiversidade
    Biochemistry
    Biochemical research methods
    Astronomia / física
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