Evidence that Nitric Oxide is Involved in the Blood Pressure Lowering Effect of the Peptide AVFQHNCQE in Spontaneously Hypertensive Rats.

Autor según el artículo: Mas-Capdevila, Anna; Iglesias-Carres, Lisard; Arola-Arnal, Anna; Aragones, Gerard; Aleixandre, Amaya; Bravo, Francisca I; Muguerza, Begona

Departamento: Bioquímica i Biotecnologia

e-ISSN: 2072-6643

Autor/es de la URV: Aragonès Bargalló, Gerard / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Muguerza Marquínez, Maria Begoña

Palabras clave: Spontaneously hypertensive rats; Rna; Rats, sprague-dawley; Rats, inbred shr; Rats; Proteins; Pro; Peptides; Oxidative stress; Nitric oxide; Mechanisms; Malondialdehyde; Male; Gene expression regulation; Endothelium, vascular; Endothelial dysfunction; Egg-white hydrolysate; Cardiovascular-diseases; Blood pressure; Bioactivity; Bioactive peptide; Aorta; Antihypertensive peptides; Antihypertensive agents; Animals; Amino acid sequence; Alpha-lactorphin; Ace-inhibitory peptides; nitric oxide; endothelial dysfunction; blood pressure; bioactivity; bioactive peptide

Resumen: AVFQHNCQE is an antihypertensive nonapeptide obtained from a chicken foot protein hydrolysate. The present study aims to investigate the mechanisms involved in its blood pressure (BP)-lowering effect. Male (17?20 weeks old) spontaneously hypertensive rats (SHR) were used in this study. Rats were divided into two groups and orally administered water or 10 mg/kg body weight (bw) AVFQHNCQE. One hour post-administration, animals of both groups were intra-peritoneally treated with 1 mL of saline or with 1 mL of saline containing 30 mg/kg bw N?-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, or with 1 mL of saline containing 5 mg/kg bw indomethacin, which is an inhibitor of prostacyclin synthesis (n = 6 per group). Systolic BP was recorded before oral administration and six hours after oral administration. In an additional experiment, SHR were administered water or 10 mg/kg bw AVFQHNCQE (n = 6 per group) and sacrificed six hours post-administration to study the mechanisms underlying the peptide anti-hypertensive effect. Moreover, the relaxation caused by AVFQHNCQE in isolated aortic rings from Sprague-Dawley rats was evaluated. The BP-lowering effect of the peptide was not changed after indomethacin administration but was completely abolished by L-NAME, which demonstrates that its anti-hypertensive effect is mediated by changes in endothelium-derived NO availability. In addition, AVFQHNCQE administration downregulated aortic gene expression of the vasoconstrictor factor endothelin-1 and the endothelial major free radical producer NADPH. Moreover, while no changes in plasma ACE activity were observed after its administration, liver GSH levels were higher in the peptide-treated group than in the water group, which demonstrates that AVFQHNCQE presents antioxidant properties.

Áreas temáticas: Zootecnia / recursos pesqueiros; Saúde coletiva; Química; Psicología; Planejamento urbano e regional / demografia; Nutrition and dietetics; Nutrition & dietetics; Nutrição; Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Food science; Farmacia; Engenharias iv; Engenharias ii; Enfermagem; Educação física; Economia; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências agrárias i; Ciência de alimentos; Biotecnología

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: franciscaisabel.bravo@urv.cat; anna.arola@urv.cat; gerard.aragones@urv.cat; begona.muguerza@urv.cat

Fecha de alta del registro: 2025-02-08

Volumen de revista: 11

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/2072-6643/11/2/225

URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Nutrients. 11 (2): e225-

Referencia de l'ítem segons les normes APA: Mas-Capdevila, Anna; Iglesias-Carres, Lisard; Arola-Arnal, Anna; Aragones, Gerard; Aleixandre, Amaya; Bravo, Francisca I; Muguerza, Begona (2019). Evidence that Nitric Oxide is Involved in the Blood Pressure Lowering Effect of the Peptide AVFQHNCQE in Spontaneously Hypertensive Rats.. Nutrients, 11(2), e225-. DOI: 10.3390/nu11020225

DOI del artículo: 10.3390/nu11020225

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2019

Tipo de publicación: Journal Publications