Autor según el artículo: Pol L; Eckstein C; Acosta L; Xifré-Pérez E; Ferré-Borrull J; Marsal L

Departamento: Enginyeria Electrònica, Elèctrica i Automàtica

e-ISSN: 2079-4991

Autor/es de la URV: Acosta Capilla, Laura Karen / Ferré Borrull, José / Marsal Garví, Luis Francisco / Xifré Pérez, Elisabet

Palabras clave: Streptavidin Rifs Nanoporous anodic alumina Biotin Biosensing rifs nanoporous anodic alumina biotin biosensing

Resumen: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores.

Áreas temáticas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)

Acceso a la licencia de uso: thttps://creativecommons.org/licenses/by/3.0/es/

ISSN: 20794991

Direcció de correo del autor: laurakaren.acosta@urv.cat lluis.marsal@urv.cat elisabet.xifre@urv.cat josep.ferre@urv.cat laurakaren.acosta@urv.cat

Identificador del autor: 0000-0002-5976-1408 0000-0001-6072-9889 0000-0002-5210-5133

Fecha de alta del registro: 2023-05-21

Volumen de revista: 9

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/2079-4991/9/3/478

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Nanomaterials. 9 (3):

Referencia de l'ítem segons les normes APA: Pol L; Eckstein C; Acosta L; Xifré-Pérez E; Ferré-Borrull J; Marsal L (2019). Real-time monitoring of biotinylated molecules detection dynamics in nanoporous anodic alumina for bio-sensing. Nanomaterials, 9(3), -. DOI: 10.3390/nano9030478

DOI del artículo: 10.3390/nano9030478

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2019

Tipo de publicación: Journal Publications