Autor según el artículo: Mendez, Lucia; Munoz, Silvia; Miralles-Perez, Bernat; Rosa Nogues, Maria; Ramos-Romero, Sara; Lluis Torres, Josep; Medina, Isabel

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: Miralles Pérez, Bernat / Nogués Llort, Maria Rosa

Palabras clave: Protein carbonylation Omega-3 pufas High-fat High sucrose diet Grape polyphenols Fish oils Diet-induced obesity omega-3 pufas high-fat, high sucrose diet grape polyphenols fish oils diet-induced obesity

Resumen: Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases and type 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damage biomolecules, especially proteins. The present study was designed to investigate the effect of marine omega-3 polyunsaturated fatty acids (PUFAs) (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) and their combination with grape seed polyphenols (GSE) on carbonyl-modified proteins from plasma and liver in Wistar Kyoto rats fed an obesogenic diet, namely high-fat and high-sucrose (HFHS) diet. A proteomics approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labelling of protein carbonyls, visualization of FTSC-labelled protein on 1-DE or 2-DE gels, and protein identification by MS/MS was used for the protein oxidation assessment. Results showed the efficiency of the combination of both bioactive compounds in decreasing the total protein carbonylation induced by HFHS diet in both plasma and liver. The analysis of carbonylated protein targets, also referred to as the 'carbonylome', revealed an individual response of liver proteins to supplements and a modulatory effect on specific metabolic pathways and processes due to, at least in part, the control exerted by the supplements on the liver protein carbonylome. This investigation highlights the additive effect of dietary fish oils and grape seed polyphenols in modulating in vivo oxidative damage of proteins induced by the consumption of HFHS diets.

Áreas temáticas: Zootecnia / recursos pesqueiros Química Pharmacology, toxicology and pharmaceutics (miscellaneous) Pharmacology & pharmacy Pharmaceutical science Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências Farmacia Engenharias iii Engenharias ii Educação física Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Biotecnología Biodiversidade Astronomia / física

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 16603397

Direcció de correo del autor: bernat.miralles@urv.cat mariarosa.nogues@urv.cat bernat.miralles@urv.cat

Identificador del autor: 0000-0003-1294-7069 0000-0003-4626-5672 0000-0003-1294-7069

Fecha de alta del registro: 2023-02-18

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/1660-3397/18/1/34

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Marine Drugs. 18 (1):

Referencia de l'ítem segons les normes APA: Mendez, Lucia; Munoz, Silvia; Miralles-Perez, Bernat; Rosa Nogues, Maria; Ramos-Romero, Sara; Lluis Torres, Josep; Medina, Isabel (2020). Modulation of the Liver Protein Carbonylome by the Combined Effect of Marine Omega-3 PUFAs and Grape Polyphenols Supplementation in Rats Fed an Obesogenic High Fat and High Sucrose Diet. Marine Drugs, 18(1), -. DOI: 10.3390/md18010034

DOI del artículo: 10.3390/md18010034

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2020

Tipo de publicación: Journal Publications