Articles producció científica> Bioquímica i Biotecnologia

Protective properties of grape-seed proanthocyanidins in human ex vivo acute colonic dysfunction induced by dextran sodium sulfate

  • Datos identificativos

    Identificador: imarina:6184563
    Autores:
    Gonzalez-Quilen, CarlosGrau-Bove, CarmeJorba-Martin, RosaCaro-Tarrago, AleidisPinent, MontserratArdevol, AnnaBeltran-Debon, RaulTerra, XimenaBlay, M Teresa
    Resumen:
    Purpose Anti-inflammatory and barrier-protective properties have been attributed to proanthocyanidins in the context of intestinal dysfunction, however little information is available about the impact of these phytochemicals on intestinal barrier integrity and immune response in the human. Here we assessed the putative protective properties of a grape-seed proanthocyanidin extract (GSPE) against dextran sodium sulfate (DSS)-induced acute dysfunction of the human colon in an Ussing chamber system. Methods Human proximal and distal colon tissues from colectomized patients were submitted ex vivo for a 30-min preventive GSPE treatment (50 or 200 mu g mL(-1)) followed by 1-h incubation with DSS (12% w v(-1)). Transepithelial electrical resistance (TEER), permeation of a fluorescently-labeled dextran (FD4) and proinflammatory cytokine release [tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta] of colonic tissues were determined. Results DSS reduced TEER (45-52%) in both the proximal and distal colon; however, significant increments in FD4 permeation (fourfold) and TNF-alpha release (61%) were observed only in the proximal colon. The preventive GSPE treatment decreased DSS-induced TEER loss (20-32%), FD4 permeation (66-73%) and TNF-alpha release (22-33%) of the proximal colon dose-dependently. The distal colon was not responsive to the preventive treatment but showed a reduction in IL-1 beta release below basal levels with the highest GSPE concentration. Conclusions Our results demonstrate potential preventive effects of GSPE on human colon dysfunction. Further studies are required to test whether administering GSPE could be a complementary therapeutic approach in colonic dysfunction associated with metabolic disorders and inflammatory bowel disease.
  • Otros:

    Autor según el artículo: Gonzalez-Quilen, Carlos; Grau-Bove, Carme; Jorba-Martin, Rosa; Caro-Tarrago, Aleidis; Pinent, Montserrat; Ardevol, Anna; Beltran-Debon, Raul; Terra, Ximena; Blay, M Teresa
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: Ardévol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GONZALEZ QUILEN, CARLOS ALBERTO / Jorba Martin, Rosa Maria / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
    Palabras clave: Vitis Ussing chamber Sulfates Sodium sulfate Signal-transduction pathways Seeds Release Rats Procyanidin Proanthocyanidins Permeability Macrophages Inflammation Humans Human colon Flavonoid Flavanols Extract Disease Dextrans Dextran sulfate Cytokines Colon Colitis procyanidin permeability inflammation human colon flavonoid
    Resumen: Purpose Anti-inflammatory and barrier-protective properties have been attributed to proanthocyanidins in the context of intestinal dysfunction, however little information is available about the impact of these phytochemicals on intestinal barrier integrity and immune response in the human. Here we assessed the putative protective properties of a grape-seed proanthocyanidin extract (GSPE) against dextran sodium sulfate (DSS)-induced acute dysfunction of the human colon in an Ussing chamber system. Methods Human proximal and distal colon tissues from colectomized patients were submitted ex vivo for a 30-min preventive GSPE treatment (50 or 200 mu g mL(-1)) followed by 1-h incubation with DSS (12% w v(-1)). Transepithelial electrical resistance (TEER), permeation of a fluorescently-labeled dextran (FD4) and proinflammatory cytokine release [tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta] of colonic tissues were determined. Results DSS reduced TEER (45-52%) in both the proximal and distal colon; however, significant increments in FD4 permeation (fourfold) and TNF-alpha release (61%) were observed only in the proximal colon. The preventive GSPE treatment decreased DSS-induced TEER loss (20-32%), FD4 permeation (66-73%) and TNF-alpha release (22-33%) of the proximal colon dose-dependently. The distal colon was not responsive to the preventive treatment but showed a reduction in IL-1 beta release below basal levels with the highest GSPE concentration. Conclusions Our results demonstrate potential preventive effects of GSPE on human colon dysfunction. Further studies are required to test whether administering GSPE could be a complementary therapeutic approach in colonic dysfunction associated with metabolic disorders and inflammatory bowel disease.
    Áreas temáticas: Serviço social Saúde coletiva Química Nutrition and dietetics Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1436-6207
    Direcció de correo del autor: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat rosamaria.jorba@urv.cat raul.beltran@urv.cat
    Identificador del autor: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378 0000-0003-3307-4340 0000-0001-9691-1906
    Fecha de alta del registro: 2024-10-19
    Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: European Journal Of Nutrition. 60 (1): 79-88
    Referencia de l'ítem segons les normes APA: Gonzalez-Quilen, Carlos; Grau-Bove, Carme; Jorba-Martin, Rosa; Caro-Tarrago, Aleidis; Pinent, Montserrat; Ardevol, Anna; Beltran-Debon, Raul; Terra, X (2021). Protective properties of grape-seed proanthocyanidins in human ex vivo acute colonic dysfunction induced by dextran sodium sulfate. European Journal Of Nutrition, 60(1), 79-88. DOI: 10.1007/s00394-020-02222-3
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Medicine (Miscellaneous),Nutrition & Dietetics,Nutrition and Dietetics
    Vitis
    Ussing chamber
    Sulfates
    Sodium sulfate
    Signal-transduction pathways
    Seeds
    Release
    Rats
    Procyanidin
    Proanthocyanidins
    Permeability
    Macrophages
    Inflammation
    Humans
    Human colon
    Flavonoid
    Flavanols
    Extract
    Disease
    Dextrans
    Dextran sulfate
    Cytokines
    Colon
    Colitis
    procyanidin
    permeability
    inflammation
    human colon
    flavonoid
    Serviço social
    Saúde coletiva
    Química
    Nutrition and dietetics
    Nutrition & dietetics
    Nutrição
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Farmacia
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
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