Articles producció científica> Química Física i Inorgànica

Nanoparticle-based mobile biosensors for the rapid detection of sepsis biomarkers in whole blood

  • Datos identificativos

    Identificador: imarina:6185849
    Handle: http://hdl.handle.net/20.500.11797/imarina6185849
  • Autores:

    Alba-Patiño A
    Russell SM
    Borges M
    Pazos-Pérez N
    Álvarez-Puebla RA
    De La Rica R
  • Otros:

    Autor según el artículo: Alba-Patiño A; Russell SM; Borges M; Pazos-Pérez N; Álvarez-Puebla RA; De La Rica R
    Departamento: Química Física i Inorgànica
    Autor/es de la URV: Alvarez Puebla, Ramon Angel / Pazos Pérez, Nicolás Carlos
    Palabras clave: Hashtag Etiqueta «#» @uroweb @residentesaeu @infoAeu
    Resumen: This journal is © The Royal Society of Chemistry. Detecting small variations in the levels of IL-6 is crucial for the early diagnosis of sepsis. To be useful in clinical decision-making, this requires detecting IL-6 rapidly in whole blood and with portable readers. Here we introduce immunosensors made of filter paper that use plasmonic nanoprobes to detect IL-6 rapidly in unprocessed blood with an unmodified smartphone. Key aspects of the biosensor fabrication were optimized in order to reduce the assay time without losing sensitivity. This included testing three bioconjugation routes for protein attachment to nanoprobes using gold nanoparticles covered with carboxylate or amine moieties, or polyvinylpyrrolidone (PVP), as starting materials, and using alternating layers of polyelectrolytes to bind the capture antibody to the paper substrate. Smartphone-based signal quantification was achieved with a custom-made app featuring a unique augmented reality guidance system that circumvents the need for smartphone attachments and automates all the steps involved in color quantification. The biosensors were able to detect IL-6 with a limit of detection of 0.1 pg mL-1 and a total assay time within 17 min. They could also detect an increase in IL-6 of only 12.5 pg mL-1 over basal levels in whole blood with 99% confidence. The high sensitivity and rapid turnaround time afforded by the optimized biosensors and the fully automated real-time densitometry app make our biosensors well suited for emergency healthcare situations such as the identification of potential sepsis cases.
    Áreas temáticas: Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General engineering General chemistry Engineering (miscellaneous) Engineering (all) Chemistry, multidisciplinary Chemistry (miscellaneous) Chemistry (all) Bioengineering Atomic and molecular physics, and optics
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 25160230
    Direcció de correo del autor: ramon.alvarez@urv.cat nicolas.pazos@urv.cat
    Identificador del autor: 0000-0003-4770-5756 0000-0002-2326-4231
    Fecha de alta del registro: 2023-02-22
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://pubs.rsc.org/en/content/articlelanding/2020/na/d0na00026d#!divAbstract
    URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Nanoscale Advances. 2 (3): 1253-1260
    Referencia de l'ítem segons les normes APA: Alba-Patiño A; Russell SM; Borges M; Pazos-Pérez N; Álvarez-Puebla RA; De La Rica R (2020). Nanoparticle-based mobile biosensors for the rapid detection of sepsis biomarkers in whole blood. Nanoscale Advances, 2(3), 1253-1260. DOI: 10.1039/d0na00026d
    DOI del artículo: 10.1039/d0na00026d
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Atomic and Molecular Physics, and Optics,Bioengineering,Chemistry (Miscellaneous),Chemistry, Multidisciplinary,Engineering (Miscellaneous),Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Nanoscience & Nanotechnology
    Nanoscience & nanotechnology
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    General materials science
    General engineering
    General chemistry
    Engineering (miscellaneous)
    Engineering (all)
    Chemistry, multidisciplinary
    Chemistry (miscellaneous)
    Chemistry (all)
    Bioengineering
    Atomic and molecular physics, and optics
  • Documentos:

  • Cerca a google

    Search to google scholar