Protective effect of the omega-3 polyunsaturated fatty acids: Eicosapentaenoic acid/Docosahexaenoic acid 1:1 ratio on cardiovascular disease risk markers in rats

Autor según el artículo: Lluís L; Taltavull N; Muñoz-Cortés M; Sánchez-Martos V; Romeu M; Giralt M; Molinar-Toribio E; Torres J; Pérez-Jiménez J; Pazos M; Méndez L; Gallardo J; Medina I; Nogués M

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: Giralt Batista, Montserrat / Muñoz Cortés, Mónica / Nogués Llort, Maria Rosa / Romeu Ferran, Marta / Sánchez Martos, Vanessa / Taltavull Anglada, Núria

Palabras clave: Triglycerides; Triacylglycerols; Supplementation; Superoxide-dismutase; Superoxide dismutase; Rats, wistar; Rats; Oxidized low density lipoprotein; Oxidative stress; Omega-3 pufa; Omega-3 polyunsaturated fatty acids (pufa); Metabolic syndrome; Malondialdehyde; Liver; Lipoproteins, ldl; Lipid peroxidation; Insulin resistance; Increases; Hypertension; Glycated hemoglobin a; Glycated hemoglobin; Glutathione reductase; Glutathione peroxidase; Fish-oil; Fish oils; Female; Erythrocytes; Eicosapentaenoic acid (epa); Eicosapentaenoic acid; Docosahexaenoic acids; Docosahexaenoic acid (dha); Diet; Controlled-trial; Cholesterol, ldl; Cholesterol, hdl; Catalase; Cardiovascular diseases; Cardiovascular disease risk; C-reactive protein; Apolipoproteins; Antioxidant status; Animals

Resumen: Background: High consumption of fish carries a lower risk of cardiovascular disease as a consequence of dietary omega-3 long chain polyunsaturated fatty acid (n-3 PUFA; especially EPA and DHA) content. A controversy exists about the component/s responsible of these beneficial effects and, in consequence, which is the best proportion between both fatty acids. We sought to determine, in healthy Wistar rats, the proportions of EPA and DHA that would induce beneficial effects on biomarkers of oxidative stress, and cardiovascular disease risk. Methods. Female Wistar rats were fed for 13 weeks with 5 different dietary supplements of oils; 3 derived from fish (EPA/DHA ratios of 1:1, 2:1, 1:2) plus soybean and linseed as controls. The activities of major antioxidant enzymes (SOD, CAT, GPX, and GR) were determined in erythrocytes and liver, and the ORAC test was used to determine the antioxidant capacity in plasma. Also measured were: C reactive protein (CRP), endothelial dysfunction (sVCAM and sICAM), prothrombotic activity (PAI-1), lipid profile (triglycerides, cholesterol, HDLc, LDLc, Apo-A1, and Apo-B100), glycated haemoglobin and lipid peroxidation (LDL-ox and MDA values). Results: After three months of nutritional intervention, we observed statistically significant differences in the ApoB100/ApoA1 ratio, glycated haemoglobin, VCAM-1, SOD and GPx in erythrocytes, ORAC values and LDL-ox. Supplementation with fish oil derived omega-3 PUFA increased VCAM-1, LDL-ox and plasma antioxidant capacity (ORAC). Conversely, the ApoB100/ApoA1 ratio and percentage glycated haemoglobin decreased. Conclusions: Our results showed that a diet of a 1:1 ratio of EPA/DHA improved many of the oxidative stress parameters (SOD and GPx in erythrocytes), plasma antioxidant capacity (ORAC) and cardiovascular risk factors (glycated haemoglobin) relative to the other diets. © 2013 Lluís et al.; licensee BioMed Central Ltd.

Áreas temáticas: Saúde coletiva; Química; Odontología; Nutrition & dietetics; Nutrição; Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; Farmacia; Ensino; Enfermagem; Endocrinology, diabetes and metabolism; Endocrinology; Educação física; Clinical biochemistry; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciência de alimentos; Biotecnología; Biodiversidade; Biochemistry (medical); Biochemistry & molecular biology

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 1476511X

Direcció de correo del autor: monica.munoz@urv.cat; nuria.taltavull@urv.cat; montse.giralt@urv.cat; vanessa.sanchez@urv.cat; vanessa.sanchez@urv.cat; marta.romeu@urv.cat

Fecha de alta del registro: 2025-01-08

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-140

URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Lipids In Health And Disease. 12 (1): 140-

Referencia de l'ítem segons les normes APA: Lluís L; Taltavull N; Muñoz-Cortés M; Sánchez-Martos V; Romeu M; Giralt M; Molinar-Toribio E; Torres J; Pérez-Jiménez J; Pazos M; Méndez L; Gallardo J (2013). Protective effect of the omega-3 polyunsaturated fatty acids: Eicosapentaenoic acid/Docosahexaenoic acid 1:1 ratio on cardiovascular disease risk markers in rats. Lipids In Health And Disease, 12(1), 140-. DOI: 10.1186/1476-511X-12-140

DOI del artículo: 10.1186/1476-511X-12-140

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2013

Tipo de publicación: Journal Publications