Autor según el artículo: Savchuk, Oleksandr; Carvajal Marti, Joan Josep; Cascales, Concepcion; Haro-Gonzalez, Patricia; Sanz-Rodriguez, Francisco; Aguilo, Magdalena; Diaz, Francesc;
Departamento: Química Física i Inorgànica
Autor/es de la URV: Aguiló Díaz, Magdalena / Carvajal Martí, Joan Josep / Díaz González, Francisco Manuel
Palabras clave: Working Upconverting nanoparticles Tm3+ Thermometry Sensitivity Platform Luminescence thermometry Hela cells Core-shell nanoparticles Biological windows Biolabeling hela cells core-shell nanoparticles biological windows biolabeling
Resumen: The bifunctional possibilities of Tm,Yb:GdVO4@SiO2 core-shell nanoparticles for temperature sensing by using the near-infrared (NIR)-excited upconversion emissions in the first biological window, and biolabeling through the visible emissions they generate, were investigated. The two emission lines located at 700 and 800 nm, that arise from the thermally coupled F-3(2,3) and H-3(4) energy levels of Tm3+, were used to develop a luminescent thermometer, operating through the Fluorescence Intensity Ratio (FIR) technique, with a very high thermal relative sensitivity. Moreover, since the inert shell surrounding the luminescent active core allows for dispersal of the nanoparticles in water and biological compatible fluids, we investigated the penetration depth that can be realized in biological tissues with their emissions in the NIR range, achieving a value of 0.8 mm when excited at powers of 50 mW. After their internalization in HeLa cells, a low toxicity was observed and the potentiality for biolabelling in the visible range was demonstrated, which facilitated the identification of the location of the nanoparticles inside the cells, and the temperature determination.
Áreas temáticas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 20794991
Direcció de correo del autor: f.diaz@urv.cat magdalena.aguilo@urv.cat joanjosep.carvajal@urv.cat
Identificador del autor: 0000-0003-4581-4967 0000-0001-6130-9579 0000-0002-4389-7298
Fecha de alta del registro: 2024-04-27
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2079-4991/10/5/993
Referencia al articulo segun fuente origial: Nanomaterials. 10 (5):
Referencia de l'ítem segons les normes APA: Savchuk, Oleksandr; Carvajal Marti, Joan Josep; Cascales, Concepcion; Haro-Gonzalez, Patricia; Sanz-Rodriguez, Francisco; Aguilo, Magdalena; Diaz, Fra (2020). Bifunctional Tm3+,Yb3+:GdVO4@SiO2 Core-Shell Nanoparticles in HeLa Cells: Upconversion Luminescence Nanothermometry in the First Biological Window and Biolabelling in the Visible. Nanomaterials, 10(5), -. DOI: 10.3390/nano10050993
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.3390/nano10050993
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Tipo de publicación: Journal Publications
Repositori URV