Autor según el artículo: Jose Valera, Maria; Zeida, Ari; Boido, Eduardo; Beltran, Gemma; Jesus Torija, Maria; Mas, Albert; Radi, Rafael; Dellacassa, Eduardo; Carrau, Francisco
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Beltran Casellas, Gemma / Mas Baron, Alberto / Torija Martínez, María Jesús / VALERA MARTÍNEZ, MARÍA JOSÉ
Palabras clave: Wine yeast Transcriptome Substrate-specificity Saccharomyces cerevisiae proteins Saccharomyces cerevisiae Pyruvate decarboxylase Phenylpyruvate decarboxylase Pathway L-phenylalanine Hanseniaspora Fermentation Diversity Crystal-structure Coenzyme q Biosynthetic pathways Benzyl alcohol Benzoylformate decarboxylase Benzene derivatives Benzaldehydes Benzaldehyde Assimilable nitrogen Aroma compounds Aro10 protein, s cerevisiae Angstrom resolution Acid 4-hydroxybenzaldehyde coenzyme q benzoylformate decarboxylase 4-hydroxybenzaldehyde
Resumen: © 2020 John Wiley & Sons, Ltd. Benzenoids are compounds associated with floral and fruity flavours in flowers, fruits and leaves and present a role in hormonal signalling in plants. These molecules are produced by the phenyl ammonia lyase pathway. However, some yeasts can also synthesize them from aromatic amino acids using an alternative pathway that remains unknown. Hanseniaspora vineae can produce benzenoids at levels up to two orders of magnitude higher than Saccharomyces species, so it is a model microorganism for studying benzenoid biosynthesis pathways in yeast. According to their genomes, several enzymes have been proposed to be involved in a mandelate pathway similar to that described for some prokaryotic cells. Among them, the ARO10 gene product could present benzoylformate decarboxylase activity. This enzyme catalyses the decarboxylation of benzoylformate into benzaldehyde at the end of the mandelate pathway in benzyl alcohol formation. Two homologous genes of ARO10 were found in the two sequenced H. vineae strains. In this study, nine other H. vineae strains were analysed to detect the presence and per cent homology of ARO10 sequences by PCR using specific primers designed for this species. Also, the copy number of the genes was estimated by quantitative PCR. To verify the relation of ARO10 with the production of benzyl alcohol during fermentation, a deletion mutant in the ARO10 gene of Saccharomyces cerevisiae was used. The two HvARO10 paralogues were analysed and compared with other α-ketoacid decarboxylases at the sequence and structural level.
Áreas temáticas: Química Odontología Mycology Microbiology Medicine (miscellaneous) Medicina ii Medicina i Genetics General medicine Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biotechnology Bioengineering Biodiversidade Biochemistry & molecular biology Biochemistry Applied microbiology and biotechnology
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 0749-503X
Direcció de correo del autor: gemma.beltran@urv.cat mjesus.torija@urv.cat albert.mas@urv.cat
Identificador del autor: 0000-0002-7071-205X 0000-0001-6419-0745 0000-0002-0763-1679
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/10.1002/yea.3508
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Yeast. 37 (9-10): 427-435
Referencia de l'ítem segons les normes APA: Jose Valera, Maria; Zeida, Ari; Boido, Eduardo; Beltran, Gemma; Jesus Torija, Maria; Mas, Albert; Radi, Rafael; Dellacassa, Eduardo; Carrau, Francisco (2020). Genetic and transcriptomic evidences suggest ARO10 genes are involved in benzenoid biosynthesis by yeast. Yeast, 37(9-10), 427-435. DOI: 10.1002/yea.3508
DOI del artículo: 10.1002/yea.3508
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2020
Tipo de publicación: Journal Publications