Autor según el artículo: Lorenzo-Gutierrez, Damaris; Gomez-Gil, Lucia; Guarro, Josep; Roncero, M. Isabel G.; Capilla, Javier; Lopez-Fernandez, Loida;

Departamento: Ciències Mèdiques Bàsiques

Autor/es de la URV: Capilla Luque, Javier

Palabras clave: P-ib-type atpase Oxidative stress Metal tolerance Intracellular-localization Homeostasis Gene Fusarium-oxysporum Fungal pathogenesis Cu homeostasis Crp Cpc motif Copper-transporter Copper (cu) transport Candida-albicans Atpase pib-type atpase cu homeostasis crp copper (cu) transport

Resumen: Cu is an essential trace element for cell growth and proliferation. However, excess of Cu accumulation leads to cellular toxicity. Thus, precise and tight regulation of Cu homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is required. Moreover, the maintenance of Cu homeostasis is critical for the survival and virulence of fungal pathogens. Cu homeostasis has been extensively studied in mammals, bacteria, and yeast, but it has not yet been well documented in filamentous fungi. In the present work, we investigated Cu tolerance in the filamentous fungusFusarium oxysporumby analysing the Cu transporter coding genecrpF, previously studied inAspergillus fumigatus. The expression studies demonstrated thatcrpFis upregulated in the presence of Cu and its deletion leads to severe sensitivity to low levels of CuSO(4)inF. oxysporum. Targeted deletion ofcrpFdid not significantly alter the resistance of the fungus to macrophage killing, nor its pathogenic behaviour on the tomato plants. However, the targeted deletion mutant Delta crpFshowed increased virulence in a murine model of systemic infection compared to wild-type strain (wt).

Áreas temáticas: Saúde coletiva Parasitology Odontología Microbiology (medical) Microbiology Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology General medicine Farmacia Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

ISSN: 21505594

Direcció de correo del autor: javier.capilla@urv.cat

Identificador del autor: 0000-0002-0765-6403

Fecha de alta del registro: 2023-02-19

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.tandfonline.com/doi/full/10.1080/21505594.2020.1809324

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Virulence. 11 (1): 1108-1121

Referencia de l'ítem segons les normes APA: Lorenzo-Gutierrez, Damaris; Gomez-Gil, Lucia; Guarro, Josep; Roncero, M. Isabel G.; Capilla, Javier; Lopez-Fernandez, Loida; (2020). Cu transporter protein CrpF protects against Cu-induced toxicity inFusarium oxysporum. Virulence, 11(1), 1108-1121. DOI: 10.1080/21505594.2020.1809324

DOI del artículo: 10.1080/21505594.2020.1809324

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2020

Tipo de publicación: Journal Publications