Articles producció científica> Ciències Mèdiques Bàsiques

Cu transporter protein CrpF protects against Cu-induced toxicity inFusarium oxysporum

  • Datos identificativos

    Identificador: imarina:8262805
    Autores:
    Lorenzo-Gutierrez, DamarisGomez-Gil, LuciaGuarro, JosepRoncero, M. Isabel G.Capilla, JavierLopez-Fernandez, Loida
    Resumen:
    Cu is an essential trace element for cell growth and proliferation. However, excess of Cu accumulation leads to cellular toxicity. Thus, precise and tight regulation of Cu homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is required. Moreover, the maintenance of Cu homeostasis is critical for the survival and virulence of fungal pathogens. Cu homeostasis has been extensively studied in mammals, bacteria, and yeast, but it has not yet been well documented in filamentous fungi. In the present work, we investigated Cu tolerance in the filamentous fungusFusarium oxysporumby analysing the Cu transporter coding genecrpF, previously studied inAspergillus fumigatus. The expression studies demonstrated thatcrpFis upregulated in the presence of Cu and its deletion leads to severe sensitivity to low levels of CuSO(4)inF. oxysporum. Targeted deletion ofcrpFdid not significantly alter the resistance of the fungus to macrophage killing, nor its pathogenic behaviour on the tomato plants. However, the targeted deletion mutant Delta crpFshowed increased virulence in a murine model of systemic infection compared to wild-type strain (wt).
  • Otros:

    Autor según el artículo: Lorenzo-Gutierrez, Damaris; Gomez-Gil, Lucia; Guarro, Josep; Roncero, M. Isabel G.; Capilla, Javier; Lopez-Fernandez, Loida;
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Capilla Luque, Javier
    Palabras clave: P-ib-type atpase Oxidative stress Metal tolerance Intracellular-localization Homeostasis Gene Fusarium-oxysporum Fungal pathogenesis Cu homeostasis Crp Cpc motif Copper-transporter Copper (cu) transport Candida-albicans Atpase pib-type atpase cu homeostasis crp copper (cu) transport
    Resumen: Cu is an essential trace element for cell growth and proliferation. However, excess of Cu accumulation leads to cellular toxicity. Thus, precise and tight regulation of Cu homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is required. Moreover, the maintenance of Cu homeostasis is critical for the survival and virulence of fungal pathogens. Cu homeostasis has been extensively studied in mammals, bacteria, and yeast, but it has not yet been well documented in filamentous fungi. In the present work, we investigated Cu tolerance in the filamentous fungusFusarium oxysporumby analysing the Cu transporter coding genecrpF, previously studied inAspergillus fumigatus. The expression studies demonstrated thatcrpFis upregulated in the presence of Cu and its deletion leads to severe sensitivity to low levels of CuSO(4)inF. oxysporum. Targeted deletion ofcrpFdid not significantly alter the resistance of the fungus to macrophage killing, nor its pathogenic behaviour on the tomato plants. However, the targeted deletion mutant Delta crpFshowed increased virulence in a murine model of systemic infection compared to wild-type strain (wt).
    Áreas temáticas: Saúde coletiva Parasitology Odontología Microbiology (medical) Microbiology Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology General medicine Farmacia Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 21505594
    Direcció de correo del autor: javier.capilla@urv.cat
    Identificador del autor: 0000-0002-0765-6403
    Fecha de alta del registro: 2023-02-19
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.tandfonline.com/doi/full/10.1080/21505594.2020.1809324
    Referencia al articulo segun fuente origial: Virulence. 11 (1): 1108-1121
    Referencia de l'ítem segons les normes APA: Lorenzo-Gutierrez, Damaris; Gomez-Gil, Lucia; Guarro, Josep; Roncero, M. Isabel G.; Capilla, Javier; Lopez-Fernandez, Loida; (2020). Cu transporter protein CrpF protects against Cu-induced toxicity inFusarium oxysporum. Virulence, 11(1), 1108-1121. DOI: 10.1080/21505594.2020.1809324
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    DOI del artículo: 10.1080/21505594.2020.1809324
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2020
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Immunology,Infectious Diseases,Microbiology,Microbiology (Medical),Parasitology
    P-ib-type atpase
    Oxidative stress
    Metal tolerance
    Intracellular-localization
    Homeostasis
    Gene
    Fusarium-oxysporum
    Fungal pathogenesis
    Cu homeostasis
    Crp
    Cpc motif
    Copper-transporter
    Copper (cu) transport
    Candida-albicans
    Atpase
    pib-type atpase
    cu homeostasis
    crp
    copper (cu) transport
    Saúde coletiva
    Parasitology
    Odontología
    Microbiology (medical)
    Microbiology
    Medicina ii
    Medicina i
    Interdisciplinar
    Infectious diseases
    Immunology
    General medicine
    Farmacia
    Engenharias i
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciência de alimentos
    Biotecnología
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