Autor según el artículo: Guerrini L; Alvarez-Puebla RA

Departamento: Química Física i Inorgànica

e-ISSN: 2079-4991

Autor/es de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca

Palabras clave: Triplex Surface-enhanced raman spectroscopy Plasmonic nanoparticles Dna

Resumen: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Direct, label-free analysis of nucleic acids via surface-enhanced Raman spectroscopy (SERS) has been continuously expanding its range of applications as an intriguing and powerful analytical tool for the structural characterization of diverse DNA structures. Still, interrogation of nucleic acid tertiary structures beyond the canonical double helix often remains challenging. In this work, we report for the first time the structural identification of DNA triplex structures. This class of nucleic acids has been attracting great interest because of their intriguing biological functions and pharmacological potential in gene therapy, and the ability for precisely engineering DNA-based functional nanomaterials. Herein, structural discrimination of the triplex structure against its du-plex and tertiary strand counterparts is univocally revealed by recognizing key markers bands in the intrinsic SERS fingerprint. These vibrational features are informative of the base stacking, Hoogsteen hydrogen bonding and sugar–phosphate backbone reorganization associated with the triple helix formation. This work expands the applicability of direct SERS to nucleic acids analysis, with potential impact on fields such as sensing, biology and drug design.

Áreas temáticas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: ramon.alvarez@urv.cat luca.guerrini@urv.cat

Identificador del autor: 0000-0003-4770-5756 0000-0002-2925-1562

Fecha de alta del registro: 2023-02-19

Volumen de revista: 11

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/2079-4991/11/2/326

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Nanomaterials. 11 (2): 1-9

Referencia de l'ítem segons les normes APA: Guerrini L; Alvarez-Puebla RA (2021). Structural recognition of triple-stranded DNA by surface-enhanced raman spectroscopy. Nanomaterials, 11(2), 1-9. DOI: 10.3390/nano11020326

DOI del artículo: 10.3390/nano11020326

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2021

Tipo de publicación: Journal Publications