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Structural recognition of triple-stranded DNA by surface-enhanced raman spectroscopy

  • Datos identificativos

    Identificador: imarina:9150163
    Autores:
    Guerrini LAlvarez-Puebla RA
    Resumen:
    © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Direct, label-free analysis of nucleic acids via surface-enhanced Raman spectroscopy (SERS) has been continuously expanding its range of applications as an intriguing and powerful analytical tool for the structural characterization of diverse DNA structures. Still, interrogation of nucleic acid tertiary structures beyond the canonical double helix often remains challenging. In this work, we report for the first time the structural identification of DNA triplex structures. This class of nucleic acids has been attracting great interest because of their intriguing biological functions and pharmacological potential in gene therapy, and the ability for precisely engineering DNA-based functional nanomaterials. Herein, structural discrimination of the triplex structure against its du-plex and tertiary strand counterparts is univocally revealed by recognizing key markers bands in the intrinsic SERS fingerprint. These vibrational features are informative of the base stacking, Hoogsteen hydrogen bonding and sugar–phosphate backbone reorganization associated with the triple helix formation. This work expands the applicability of direct SERS to nucleic acids analysis, with potential impact on fields such as sensing, biology and drug design.
  • Otros:

    Autor según el artículo: Guerrini L; Alvarez-Puebla RA
    Departamento: Química Física i Inorgànica
    e-ISSN: 2079-4991
    Autor/es de la URV: Alvarez Puebla, Ramon Angel / Guerrini, Luca
    Palabras clave: Triplex Surface-enhanced raman spectroscopy Plasmonic nanoparticles Dna
    Resumen: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Direct, label-free analysis of nucleic acids via surface-enhanced Raman spectroscopy (SERS) has been continuously expanding its range of applications as an intriguing and powerful analytical tool for the structural characterization of diverse DNA structures. Still, interrogation of nucleic acid tertiary structures beyond the canonical double helix often remains challenging. In this work, we report for the first time the structural identification of DNA triplex structures. This class of nucleic acids has been attracting great interest because of their intriguing biological functions and pharmacological potential in gene therapy, and the ability for precisely engineering DNA-based functional nanomaterials. Herein, structural discrimination of the triplex structure against its du-plex and tertiary strand counterparts is univocally revealed by recognizing key markers bands in the intrinsic SERS fingerprint. These vibrational features are informative of the base stacking, Hoogsteen hydrogen bonding and sugar–phosphate backbone reorganization associated with the triple helix formation. This work expands the applicability of direct SERS to nucleic acids analysis, with potential impact on fields such as sensing, biology and drug design.
    Áreas temáticas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: ramon.alvarez@urv.cat luca.guerrini@urv.cat
    Identificador del autor: 0000-0003-4770-5756 0000-0002-2925-1562
    Fecha de alta del registro: 2024-07-27
    Volumen de revista: 11
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2079-4991/11/2/326
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Nanomaterials. 11 (2): 1-9
    Referencia de l'ítem segons les normes APA: Guerrini L; Alvarez-Puebla RA (2021). Structural recognition of triple-stranded DNA by surface-enhanced raman spectroscopy. Nanomaterials, 11(2), 1-9. DOI: 10.3390/nano11020326
    DOI del artículo: 10.3390/nano11020326
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Chemical Engineering (Miscellaneous),Chemistry, Multidisciplinary,Materials Science (Miscellaneous),Materials Science, Multidisciplinary,Nanoscience & Nanotechnology,Physics, Applied
    Triplex
    Surface-enhanced raman spectroscopy
    Plasmonic nanoparticles
    Dna
    Physics, applied
    Nanoscience & nanotechnology
    Materials science, multidisciplinary
    Materials science (miscellaneous)
    Materials science (all)
    General materials science
    General chemical engineering
    Engenharias ii
    Chemistry, multidisciplinary
    Chemical engineering (miscellaneous)
    Chemical engineering (all)
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