Articles producció científica> Bioquímica i Biotecnologia

A pilot study for metabolic profiling of obesity-associated microbial gut dysbiosis in male wistar rats

  • Datos identificativos

    Identificador: imarina:9162275
    Autores:
    Hernandez-Baixauli JPuigbò PTorrell HPalacios-Jordan HRipoll VJRCaimari ABas JMDBaselga-Escudero LMulero M
    Resumen:
    © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Obesity is one of the most incident and concerning disease worldwide. Definite strategies to prevent obesity and related complications remain elusive. Among the risk factors of the onset of obesity, gut microbiota might play an important role in the pathogenesis of the disease, and it has received extensive attention because it affects the host metabolism. In this study, we aimed to define a metabolic profile of the segregated obesity-associated gut dysbiosis risk factor. The study of the metabolome, in an obesity-associated gut dysbiosis model, provides a relevant way for the discrim-ination on the different biomarkers in the obesity onset. Thus, we developed a model of this obesity risk factors through the transference of gut microbiota from obese to non-obese male Wistar rats and performed a subsequent metabolic analysis in the receptor rats. Our results showed alterations in the lipid metabolism in plasma and in the phenylalanine metabolism in urine. In consequence, we have identified metabolic changes characterized by: (1) an increase in DG:34:2 in plasma, a decrease in hippurate, (2) an increase in 3-HPPA, and (3) an increase in o-coumaric acid. Hereby, we propose these metabolites as a metabolic profile associated to a segregated dysbiosis state related to obesity disease.
  • Otros:

    Autor según el artículo: Hernandez-Baixauli J; Puigbò P; Torrell H; Palacios-Jordan H; Ripoll VJR; Caimari A; Bas JMD; Baselga-Escudero L; Mulero M
    Departamento: Bioquímica i Biotecnologia
    Autor/es de la URV: BASELGA ESCUDERO, LAURA / Mulero Abellán, Miguel / PUIGBÒ AVALOS, PEDRO
    Palabras clave: O-coumaric acid Microbial dysbiosis Metagenomics Metabolomics Metabolic profile Hippurate Gut microbiota Dysbiosis biomarkers Dysbiosis bi-omarkers Diacylglycerol 34:2 3-hppa
    Resumen: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Obesity is one of the most incident and concerning disease worldwide. Definite strategies to prevent obesity and related complications remain elusive. Among the risk factors of the onset of obesity, gut microbiota might play an important role in the pathogenesis of the disease, and it has received extensive attention because it affects the host metabolism. In this study, we aimed to define a metabolic profile of the segregated obesity-associated gut dysbiosis risk factor. The study of the metabolome, in an obesity-associated gut dysbiosis model, provides a relevant way for the discrim-ination on the different biomarkers in the obesity onset. Thus, we developed a model of this obesity risk factors through the transference of gut microbiota from obese to non-obese male Wistar rats and performed a subsequent metabolic analysis in the receptor rats. Our results showed alterations in the lipid metabolism in plasma and in the phenylalanine metabolism in urine. In consequence, we have identified metabolic changes characterized by: (1) an increase in DG:34:2 in plasma, a decrease in hippurate, (2) an increase in 3-HPPA, and (3) an increase in o-coumaric acid. Hereby, we propose these metabolites as a metabolic profile associated to a segregated dysbiosis state related to obesity disease.
    Áreas temáticas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: miquel.mulero@urv.cat
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2218-273X/11/2/303
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Biomolecules. 11 (2): 1-22
    Referencia de l'ítem segons les normes APA: Hernandez-Baixauli J; Puigbò P; Torrell H; Palacios-Jordan H; Ripoll VJR; Caimari A; Bas JMD; Baselga-Escudero L; Mulero M (2021). A pilot study for metabolic profiling of obesity-associated microbial gut dysbiosis in male wistar rats. Biomolecules, 11(2), 1-22. DOI: 10.3390/biom11020303
    DOI del artículo: 10.3390/biom11020303
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry,Biochemistry & Molecular Biology,Molecular Biology
    O-coumaric acid
    Microbial dysbiosis
    Metagenomics
    Metabolomics
    Metabolic profile
    Hippurate
    Gut microbiota
    Dysbiosis biomarkers
    Dysbiosis bi-omarkers
    Diacylglycerol 34:2
    3-hppa
    Química
    Molecular biology
    Materiais
    General medicine
    Farmacia
    Ensino
    Biochemistry & molecular biology
    Biochemistry
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