Impact of gut microbiota on plasma oxylipins profile under healthy and obesogenic conditions

Autor según el artículo: Avila-Roman, Javier; Arreaza-Gil, Veronica; Cortes-Espinar, Antonio J; Soliz-Rueda, Jorge R; Mulero, Miquel; Muguerza, Begona; Arola-Arnal, Anna; Arola, Lluis; Torres-Fuentes, Cristina

Departamento: Bioquímica i Biotecnologia

Autor/es de la URV: Arola Arnal, Anna / Arola Ferrer, Luis Maria / Arreaza Gil, Verónica / Ávila Román, Francisco Javier / Cortés Espinar, Antonio Jesús / Muguerza Marquínez, Maria Begoña / Mulero Abellán, Miguel / Soliz Rueda, Jorge Ricardo / Torres Fuentes, Cristina

Palabras clave: Resolution; Rats, wistar; Rats; Oxylipins; Obesity; Metabolomics; Metabolic syndrome; Mechanisms; Male; Lipid mediators; Inflammation; Gut microbiota; Germ-free; Gastrointestinal microbiome; Dysbiosis; Disease models, animal; Dietary linoleic-acid; Diet; Cafeteria diet; Biomarkers; Bacteria; Antibiotics; Anti-bacterial agents; Animals; Accumulation

Resumen: © 2021 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism Background & aims: Oxylipins (OXLs) are bioactive lipid metabolites derived from polyunsaturated fatty acids (PUFAs) which act as signaling molecules and are involved in inflammatory processes such as those that occur in obesity. On the other hand, gut microbiota plays an essential role in regulating inflammatory responses. However, little is known about the potential impact of gut bacteria on OXLs metabolism. Thus, the objective of this study was to investigate the effect of gut microbiota dysbiosis on plasma oxylipins profile in healthy and diet-induced obese animals. Methods: Eight-week-old male Wistar rats were fed with either a standard or cafeteria diet (CAF) for 5 weeks and administered an antibiotic cocktail (ABX) in the drinking water (Ampicillin: 1 g/ml, Vancomycin: 0.5 g/ml, Imipenem: 0.25 g/ml) for the last 2 weeks in order to induce gut microbiota dysbiosis. Metabolomics analysis of OXLs in plasma was performed by HPLC–MS analysis. No antibiotic treated animals were included as controls. Results: Plasma OXLs profile was significantly altered due to both CAF feeding and ABX administration. ABX effect was more pronounced under obesogenic conditions. Several significant correlations between different bacteria taxa and these lipid mediators were observed. Among these, the positive correlation of Proteobacteria with LTB4, a proinflammatory OXL involved in obesity-related disorders, was especially remarkable. Conclusions: Gut microbiota plays a key role in regulating these lipid metabolites and, therefore, affecting oxylipins-mediated inflammatory processes. These results are the first evidence to our knowledge of gut microbiota impact on OXLs metabolism. Moreover, this can set the basis for developing new obesity markers based on OXLs and gut microbiota profiles.

Áreas temáticas: Saúde coletiva; Química; Odontología; Nutrition and dietetics; Nutrition & dietetics; Nutrição; Medicina iii; Medicina ii; Medicina i; Interdisciplinar; General medicine; Farmacia; Engenharias iv; Enfermagem; Educação física; Critical care and intensive care medicine; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciência de alimentos; Biotecnología

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: antoniojesus.cortes@urv.cat; franciscojavier.avila@urv.cat; jorgericardo.soliz@urv.cat; antoniojesus.cortes@urv.cat; antoniojesus.cortes@urv.cat; veronica.arreaza@urv.cat; veronica.arreaza@urv.cat; veronica.arreaza@urv.cat; miquel.mulero@urv.cat; anna.arola@urv.cat; lluis.arola@urv.cat; begona.muguerza@urv.cat; cristina.torres@urv.cat

Fecha de alta del registro: 2025-01-28

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.clinicalnutritionjournal.com/article/S0261-5614(21)00123-0/fulltext#%20

URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Clinical Nutrition. 40 (4): 1475-1486

Referencia de l'ítem segons les normes APA: Avila-Roman, Javier; Arreaza-Gil, Veronica; Cortes-Espinar, Antonio J; Soliz-Rueda, Jorge R; Mulero, Miquel; Muguerza, Begona; Arola-Arnal, Anna; Arol (2021). Impact of gut microbiota on plasma oxylipins profile under healthy and obesogenic conditions. Clinical Nutrition, 40(4), 1475-1486. DOI: 10.1016/j.clnu.2021.02.035

DOI del artículo: 10.1016/j.clnu.2021.02.035

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2021

Tipo de publicación: Journal Publications