Autor según el artículo: Murciano, Celia; Lee, Chung-Te; Fernandez-Bravo, Ana; Hsieh, Tsung-Han; Fouz, Belen; Hor, Lien-I; Amaro, Carmen;
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: Fernández Bravo, Ana
Palabras clave: Virulence plasmid Vibrio V. vulnificus Sepsis Rtx toxin Qpcr array Pathogen Metalloprotease Martx Infection Identification Gene expression Escherichia-coli Domain Biotype 2 Bacteria
Resumen: Vibrio vulnificus biotype 2-serovar E is a zoonotic clonal complex that can cause death by sepsis in humans and fish. Unlike other biotypes, Bt2 produces a unique type of MARTX(Vv) (Multifunctional-Autoprocessive-Repeats-in-Toxin; RtxA1(3)), which is encoded by a gene duplicated in the pVvBt2 plasmid and chromosome II. In this work, we analyzed the activity of this toxin and its role in human sepsis by performing in vitro, ex vivo, and in vivo assays. First, we demonstrated that the ACD domain, present exclusively in this toxin variant, effectively has an actin-cross-linking activity. Second, we determined that the whole toxin caused death of human endotheliocytes and monocytes by lysis and apoptosis, respectively. Finally, we tested the hypothesis that RtxA1(3) contributes to human death caused by this zoonotic serovar by triggering an early cytokine storm in blood. To this end, we used a Bt2-SerE strain (R99) together with its rtxA1(3) deficient mutant, and a Bt1 strain (YJ016) producing RtxA1(1) (the most studied MARTX(Vv)) together with its rtxA1(1) deficient mutant, as controls. Our results showed that RtxA1(3) was essential for virulence, as R99 Delta Delta rtxA1(3) was completely avirulent in our murine model of infection, and that R99, but not strain YJ016, induced an early, strong and dysregulated immune response involving the up-regulation of a high number of genes. This dysregulated immune response was directly linked to RtxA1(3). Based on these results and those obtained ex vivo (human blood), we propose a model of infection for the zoonotic serovar of V. vulnificus, in which RtxA1(3) would act as a sepsis-inducing toxin.
Áreas temáticas: Saúde coletiva Química Odontología Microbiology (medical) Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology Geociências Farmacia Ensino Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: ana.fernandez@urv.cat
Identificador del autor: 0000-0001-7637-5958
Fecha de alta del registro: 2024-09-07
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.frontiersin.org/articles/10.3389/fcimb.2017.00332/full
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Frontiers In Cellular And Infection Microbiology. 7
Referencia de l'ítem segons les normes APA: Murciano, Celia; Lee, Chung-Te; Fernandez-Bravo, Ana; Hsieh, Tsung-Han; Fouz, Belen; Hor, Lien-I; Amaro, Carmen; (2017). MARTX Toxin in the Zoonotic Serovar of Vibrio vulnificus Triggers an Early Cytokine Storm in Mice. Frontiers In Cellular And Infection Microbiology, 7(), -. DOI: 10.3389/fcimb.2017.00332
DOI del artículo: 10.3389/fcimb.2017.00332
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2017
Tipo de publicación: Journal Publications