Autor según el artículo: Sok, Vibol; Fragoso, Alex;

Departamento: Enginyeria Química

Autor/es de la URV: Fragoso Sierra, Alex / SOK, VIBOL

Palabras clave: Phenoxy herbicide Pesticides Peroxidase Immunosensors Horseradish-peroxidase Glyphosate Electrochemical biosensor Electrochemical biosen-sor Cyclodextrin Carbon nano-onion 5-t 4-d 4 2,4-dichlorophenoxyacetic acid 2,4-d 2,4,5-t 2

Resumen: Featured Application Electrochemical detection of phenoxy herbicides based on peroxidase inhibition using a carbon nano-onion modified biosensor. Carbon nano-onions are emerging electrode materials in biosensing due to their high conductivity and biocompatibility. Phenoxy-based herbicides are a source of environmental contamination that can be detected using their property to inhibit the activity of some enzymes. Here we report a biosensor based on peroxidase immobilized on carbon nano-onions in a cyclodextrin polymer matrix for the amperometric detection of 2,4-D and 2,4,5-T. The inhibition mechanism of 2,4-D and 2,4,5-T on peroxidase activity was first elucidated by activity measurements and molecular docking. The biosensor was characterized by electrochemical and microscopy methods and applied to the amperometric detection of these herbicides. The incorporation of carbon nano-onions enhanced the sensitivity of the biosensor and improved its stability and repeatability. The application of the developed biosensor to the detection of 2,4-D in soil and 2,4,5-T in river water samples is also reported.

Áreas temáticas: Química Process chemistry and technology Physics, applied Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) Materiais Instrumentation General materials science General engineering Fluid flow and transfer processes Engineering, multidisciplinary Engineering (miscellaneous) Engineering (all) Engenharias ii Engenharias i Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Chemistry, multidisciplinary Biodiversidade Astronomia / física

Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/

Direcció de correo del autor: alex.fragoso@urv.cat

Identificador del autor: 0000-0003-4839-6094

Fecha de alta del registro: 2023-02-23

Volumen de revista: 11

Versión del articulo depositado: info:eu-repo/semantics/publishedVersion

Enlace a la fuente original: https://www.mdpi.com/2076-3417/11/15/6889

URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/

Referencia al articulo segun fuente origial: Applied Sciences-Basel. 11 (15):

Referencia de l'ítem segons les normes APA: Sok, Vibol; Fragoso, Alex; (2021). Carbon Nano-Onion Peroxidase Composite Biosensor for Electrochemical Detection of 2,4-D and 2,4,5-T. Applied Sciences-Basel, 11(15), -. DOI: 10.3390/app11156889

DOI del artículo: 10.3390/app11156889

Entidad: Universitat Rovira i Virgili

Año de publicación de la revista: 2021

Tipo de publicación: Journal Publications