Autor según el artículo: Sánchez-Ceinos J; Guzmán-Ruiz R; Rangel-Zúñiga OA; López-Alcalá J; Moreno-Caño E; Del Río-Moreno M; Romero-Cabrera JL; Pérez-Martínez P; Maymo-Masip E; Vendrell J; Fernández-Veledo S; Fernández-Real JM; Laurencikiene J; Rydén M; Membrives A; Luque RM; López-Miranda J; Malagón MM
Departamento: Medicina i Cirurgia Bioquímica i Biotecnologia
Autor/es de la URV: Fernandez Veledo, Sonia / Maymo Masip, Elsa / Vendrell Ortega, Juan José
Palabras clave: Preadipocytes Human adipose-tissue upr tool stress rna splicing obesity-related metabolic diseases insulin-resistance human adipocytes human homeostasis fat erad er-proteostasis differentiation cells adipogenesis
Resumen: Preadipocytes are crucial for healthy adipose tissue expansion. Preadipocyte differentiation is altered in obese individuals, which has been proposed to contribute to obesity-associated metabolic disturbances. Here, we aimed at identifying the pathogenic processes underlying impaired adipocyte differentiation in obese individuals with insulin resistance (IR)/type 2 diabetes (T2D). We report that down-regulation of a key member of the major spliceosome, PRFP8/PRP8, as observed in IR/T2D preadipocytes from subcutaneous (SC) fat, prevented adipogenesis by altering both the expression and splicing patterns of adipogenic transcription factors and lipid droplet-related proteins, while adipocyte differentiation was restored upon recovery of PRFP8/PRP8 normal levels. Adipocyte differentiation was also compromised under conditions of endoplasmic reticulum (ER)-associated protein degradation (ERAD) hyperactivation, as occurs in SC and omental (OM) preadipocytes in IR/T2D obesity. Thus, targeting mRNA splicing and ER proteostasis in preadipocytes could improve adipose tissue function and thus contribute to metabolic health in obese individuals.
Áreas temáticas: Saúde coletiva Química Nutrição Neuroscience (miscellaneous) Neuroscience (all) Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Immunology and microbiology (miscellaneous) Immunology and microbiology (all) Geografía Geociências General neuroscience General medicine General immunology and microbiology General biochemistry,genetics and molecular biology Farmacia Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biology Biodiversidade Biochemistry, genetics and molecular biology (miscellaneous) Biochemistry, genetics and molecular biology (all) Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: sonia.fernandez@urv.cat elsa.maymo@urv.cat juanjose.vendrell@urv.cat
Identificador del autor: 0000-0003-2906-3788 0000-0002-9133-3120 0000-0002-6994-6115
Fecha de alta del registro: 2024-07-27
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Elife. 10
Referencia de l'ítem segons les normes APA: Sánchez-Ceinos J; Guzmán-Ruiz R; Rangel-Zúñiga OA; López-Alcalá J; Moreno-Caño E; Del Río-Moreno M; Romero-Cabrera JL; Pérez-Martínez P; Maymo-Masip E (2021). Impaired mRNA splicing and proteostasis in preadipocytes in obesity related metabolic disease. Elife, 10(), -. DOI: 10.7554/eLife.65996
DOI del artículo: 10.7554/eLife.65996
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2021
Tipo de publicación: Journal Publications
Repositori URV