Articles producció científica> Enginyeria Química

Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish

  • Datos identificativos

    Identificador: imarina:9230993
    Autores:
    Shkembi, XhensilaSkouridou, VassoSvobodova, MarketaLeonardo, SandraBashammakh, Abdulaziz SAlyoubi, Abdulrahman OCampas, MonicaO'Sullivan, Ciara K
    Resumen:
    The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody-aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.
  • Otros:

    Autor según el artículo: Shkembi, Xhensila; Skouridou, Vasso; Svobodova, Marketa; Leonardo, Sandra; Bashammakh, Abdulaziz S; Alyoubi, Abdulrahman O; Campas, Monica; O'Sullivan, Ciara K
    Departamento: Enginyeria Química
    Autor/es de la URV: CAMPAS HOMS, MONICA / O'SULLIVAN, CIARA KATHLEEN / Shkembi, Xhensila / Skouridou, Vasoula / SVOBODOVÁ, MARKÉTA
    Palabras clave: Toxic materials Tetrodotoxin Tetraodontiformes Surface-plasmon resonance Reliable detection Pufferfish Prevention strategies Mass spectrometry Marine toxins Marine species Liquid chromatography - mass spectrometries Liquid chromatography Immunology Immunoassay Health safety Health risks Fast detections Cost effectiveness Chromatography, liquid Chemical detection Binding energy Aptamers Aptamer assay Antibodies Animals ttx toxicity selection lagocephalus-sceleratus immunoassay fish dna-aptamers capture-selex 1st detection
    Resumen: The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody-aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.
    Áreas temáticas: Química Medicina ii Medicina i Materiais Interdisciplinar Geociências General medicine Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Enfermagem Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: vasoula.skouridou@urv.cat monica.campas@urv.cat xhensila.shkembi@estudiants.urv.cat
    Identificador del autor: 0000-0002-9712-5429 0000-0002-1220-7100
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://pubs.acs.org/doi/10.1021/acs.analchem.1c03671
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Analytical Chemistry. 93 (44): 14810-14819
    Referencia de l'ítem segons les normes APA: Shkembi, Xhensila; Skouridou, Vasso; Svobodova, Marketa; Leonardo, Sandra; Bashammakh, Abdulaziz S; Alyoubi, Abdulrahman O; Campas, Monica; O'Sullivan (2021). Hybrid Antibody–Aptamer Assay for Detection of Tetrodotoxin in Pufferfish. Analytical Chemistry, 93(44), 14810-14819. DOI: 10.1021/acs.analchem.1c03671
    DOI del artículo: 10.1021/acs.analchem.1c03671
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2021
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Analytical Chemistry,Chemistry, Analytical
    Toxic materials
    Tetrodotoxin
    Tetraodontiformes
    Surface-plasmon resonance
    Reliable detection
    Pufferfish
    Prevention strategies
    Mass spectrometry
    Marine toxins
    Marine species
    Liquid chromatography - mass spectrometries
    Liquid chromatography
    Immunology
    Immunoassay
    Health safety
    Health risks
    Fast detections
    Cost effectiveness
    Chromatography, liquid
    Chemical detection
    Binding energy
    Aptamers
    Aptamer assay
    Antibodies
    Animals
    ttx
    toxicity
    selection
    lagocephalus-sceleratus
    immunoassay
    fish
    dna-aptamers
    capture-selex
    1st detection
    Química
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Geociências
    General medicine
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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