Articles producció científica> Medicina i Cirurgia

Characterization of the lps and 3ohfa contents in the lipoprotein fractions and lipoprotein particles of healthy men

  • Datos identificativos

    Identificador: imarina:9242336
    Autores:
    Rehues, PRodríguez, MAlvarez, JJiménez, MMelià, ASempere, MBalsells, CCastillejo, GGuardiola, MCastro, ARibalta, J
    Resumen:
    Atherosclerosis is a chronic inflammatory disease that is caused by the accumulation of LDL particles in the intima, causing the activation of immune cells and triggering an inflammatory response. LPS is a potent activator of the innate immune response and it can be transported by lipoproteins. Since humans are much more sensitive to LPS than other mammals, and very low amounts of LPS can elicit an immune response, the aim of this study is to characterize the distribution of LPS and its immunogenic portion (3OHFAs) among lipoprotein types of healthy men. We separated lipoprotein fractions by ultracentrifugation and the amount of each 3OHFA was measured by MS in each lipoprotein fraction to calculate LPS concentration. Lipoprotein particle concentration was measured by NMR. LDL and HDL fractions transported the highest concentration of LPS (35.7% and 31.5%, respectively), but VLDL particles carried more LPS molecules per particle (0.55 molecules/particle) than LDL or HDL (p < 0.01). The distribution of LPS and all 3OHFAs among lipoprotein fractions showed high interindividual variability, suggesting that they may be studied as a potential biomarker. This may help understand the role of LPS in atherosclerosis in those cases where the disease cannot be explained by traditional risk factors. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
  • Otros:

    Autor según el artículo: Rehues, P; Rodríguez, M; Alvarez, J; Jiménez, M; Melià, A; Sempere, M; Balsells, C; Castillejo, G; Guardiola, M; Castro, A; Ribalta, J
    Departamento: Medicina i Cirurgia
    Autor/es de la URV: Alvarez Vicente, Juan Jose / Balsells Llauradò, Clara / Castillejo De Villasante, Gemma / Castro Salomó, Antoni / Guardiola Guionnet, Montserrat / Jiménez Herrera, María Francisca / Rehues Masip, Pere / Ribalta Vives, Josep / Rodríguez Chacón, Matilde
    Palabras clave: Lipoprotein Lipopolysaccharide Atherosclerosis Adipose-tissue 3-hydroxy fatty acids plasma lipoprotein lipopolysaccharide kinetics expression endotoxin cholesterol binding atherosclerosis association 3-hydroxy fatty acids
    Resumen: Atherosclerosis is a chronic inflammatory disease that is caused by the accumulation of LDL particles in the intima, causing the activation of immune cells and triggering an inflammatory response. LPS is a potent activator of the innate immune response and it can be transported by lipoproteins. Since humans are much more sensitive to LPS than other mammals, and very low amounts of LPS can elicit an immune response, the aim of this study is to characterize the distribution of LPS and its immunogenic portion (3OHFAs) among lipoprotein types of healthy men. We separated lipoprotein fractions by ultracentrifugation and the amount of each 3OHFA was measured by MS in each lipoprotein fraction to calculate LPS concentration. Lipoprotein particle concentration was measured by NMR. LDL and HDL fractions transported the highest concentration of LPS (35.7% and 31.5%, respectively), but VLDL particles carried more LPS molecules per particle (0.55 molecules/particle) than LDL or HDL (p < 0.01). The distribution of LPS and all 3OHFAs among lipoprotein fractions showed high interindividual variability, suggesting that they may be studied as a potential biomarker. This may help understand the role of LPS in atherosclerosis in those cases where the disease cannot be explained by traditional risk factors. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
    Áreas temáticas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: pere.rehues@urv.cat gemma.castillejo@urv.cat matilde.rodriguez@urv.cat juanjose.alvarez@estudiants.urv.cat pere.rehues@urv.cat antoni.castro@urv.cat montse.guardiola@urv.cat maria.jimenez@urv.cat josep.ribalta@urv.cat
    Identificador del autor: 0000-0001-5441-6333 0000-0002-9696-7384 0000-0003-2599-3742 0000-0002-8879-4719
    Fecha de alta del registro: 2024-09-07
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.mdpi.com/2218-273X/12/1/47
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Biomolecules. 12 (1):
    Referencia de l'ítem segons les normes APA: Rehues, P; Rodríguez, M; Alvarez, J; Jiménez, M; Melià, A; Sempere, M; Balsells, C; Castillejo, G; Guardiola, M; Castro, A; Ribalta, J (2022). Characterization of the lps and 3ohfa contents in the lipoprotein fractions and lipoprotein particles of healthy men. Biomolecules, 12(1), -. DOI: 10.3390/biom12010047
    DOI del artículo: 10.3390/biom12010047
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2022
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry,Biochemistry & Molecular Biology,Molecular Biology
    Lipoprotein
    Lipopolysaccharide
    Atherosclerosis
    Adipose-tissue
    3-hydroxy fatty acids
    plasma
    lipoprotein
    lipopolysaccharide
    kinetics
    expression
    endotoxin
    cholesterol
    binding
    atherosclerosis
    association
    3-hydroxy fatty acids
    Química
    Molecular biology
    Materiais
    General medicine
    Farmacia
    Ensino
    Biochemistry & molecular biology
    Biochemistry
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