Autor según el artículo: Colom-Pellicer, Marina; Rodriguez, Romina M.; Navarro-Masip, Elia; Bravo, Francisca Isabel; Mulero, Miquel; Arola, Lluis; Aragones, Gerard;
Departamento: Bioquímica i Biotecnologia
Autor/es de la URV: Aragonès Bargalló, Gerard / Arola Ferrer, Luis Maria / Bravo Vázquez, Francisca Isabel / Colom Pellicer, Marina / Mulero Abellán, Miguel / Navarro Masip, Èlia / RODRÍGUEZ SÁNCHEZ, ROSA MARIA
Palabras clave: Transplantation Mice Healthy Fat Clocks
Resumen: Background Grape-seed proanthocyanidin extract (GSPE) improve white adipose tissue (WAT) expansion during diet-induced obesity. However, because adipose metabolism is synchronized by circadian rhythms, it is plausible to speculate that the bioactivity of dietary proanthocyanidins could be influenced by the time-of-day in which they are consumed. Therefore, the aim of the present study was to determine the interaction between zeitgeber time (ZT) and GSPE consumption on the functionality of WAT in rats with diet-induced obesity. Methods Male Wistar rats were fed a cafeteria diet for 9 weeks. After 5 weeks, the animals were supplemented with 25 mg GSPE/kg for 4 weeks at the beginning of the light/rest phase (ZT0) or of the dark/active phase (ZT12). Body fat content was determined by nuclear magnetic resonance and histological analyses were performed in the epididymal (EWAT) and inguinal (IWAT) fat depots to determine adipocyte size and number. In addition, the expression of genes related to adipose metabolism and circadian clock function were analyzed by qPCR. Results GSPE consumption at ZT0 was associated with a potential antidiabetic effect without affecting adiposity and energy intake and downregulating the gene expression of inflammatory markers in EWAT. In contrast, GSPE consumption at ZT12 improved adipose tissue expansion decreasing adipocyte size in IWAT. In accordance with this adipogenic activity, the expression of genes involved in fatty acid metabolism were downregulated at ZT12 in IWAT. In turn, GSPE consumption at ZT12, but not at ZT0, repressed the expression of the clock gene Cry1 in IWAT. Conclusions The interaction between ZT and GSPE consumption influenced the metabolic response of WAT in a tissue-specific manner. Understanding the impact of circadian clock on adipose metabolism and how this is regulated by polyphenols will provide new insights for the management of obesity.
Áreas temáticas: Serviço social Saúde coletiva Psicología Nutrition and dietetics Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: marina.colom@urv.cat marina.colom@urv.cat marina.colom@urv.cat elia.navarro@estudiants.urv.cat elia.navarro@estudiants.urv.cat franciscaisabel.bravo@urv.cat miquel.mulero@urv.cat gerard.aragones@urv.cat lluis.arola@urv.cat
Identificador del autor: 0000-0002-6468-3088 0000-0003-2767-1974
Fecha de alta del registro: 2024-09-21
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://www.nature.com/articles/s41366-022-01132-0
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: International Journal Of Obesity. 46 (7): 1394-1402
Referencia de l'ítem segons les normes APA: Colom-Pellicer, Marina; Rodriguez, Romina M.; Navarro-Masip, Elia; Bravo, Francisca Isabel; Mulero, Miquel; Arola, Lluis; Aragones, Gerard; (2022). Time-of-day dependent effect of proanthocyanidins on adipose tissue metabolism in rats with diet-induced obesity. International Journal Of Obesity, 46(7), 1394-1402. DOI: 10.1038/s41366-022-01132-0
DOI del artículo: 10.1038/s41366-022-01132-0
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications
Repositori URV