Autor según el artículo: Linklater DP; Le Guével X; Bryant G; Baulin VA; Pereiro E; Perera PGT; Wandiyanto JV; Juodkazis S; Ivanova EP
Departamento: Química Física i Inorgànica
Autor/es de la URV: Baulin, Vladimir
Palabras clave: Ultrasmall metal nanoclusters Translocation Nanotoxicity In-vitro Gold nanoclusters Antimicrobial effect translocation toxicity tomography size shape nanotoxicity nanoparticles morphology membranes gold nanoclusters antimicrobial effect
Resumen: Ultrasmall metal nanoclusters (NCs) are employed in an array of diagnostic and therapeutic applications due to their tunable photoluminescence, high biocompatibility, polyvalent effect, ease of modification, and photothermal stability. However, gold nanoclusters' (AuNCs') intrinsically antimicrobial properties remain poorly explored and are not well understood. Here, we share an insight into the antimicrobial action of atomically precise AuNCs based on their ability to passively translocate across the bacterial membrane. Functionalized by a hydrophilic modified-bidentate sulfobetaine zwitterionic molecule (AuNC-ZwBuEt) or a more hydrophobic monodentate-thiolate, mercaptohexanoic acid (AuNC-MHA) molecule, 2 nm AuNCs were lethal to both Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. The bactericidal efficiency was found to be bacterial strain-, time-, and concentration-dependent. The direct visualizations of the translocation of AuNCs and AuNC-cell and subcellular interactions were investigated using cryo-soft X-ray nano-tomography, transmission electron microscopy (TEM), and scanning TEM energy-dispersive spectroscopy analyses. AuNC-MHA were identified in the bacterial cytoplasm within 30 min, without evidence of the loss of membrane integrity. It is proposed that the bactericidal effect of AuNCs is attributed to their size, which allows for efficient energy-independent translocation across the cell membrane. The internalization of both AuNCs caused massive internal damage to the cells, including collapsed subcellular structures and altered cell morphology, leading to the eventual loss of cellular integrity.
Áreas temáticas: Química Nanoscience and nanotechnology Nanoscience & nanotechnology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) Materiais Interdisciplinar General materials science Farmacia Engenharias iv Engenharias iii Engenharias ii Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Biodiversidade Astronomia / física Arquitetura, urbanismo e design
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: vladimir.baulin@urv.cat
Identificador del autor: 0000-0003-2086-4271
Fecha de alta del registro: 2023-02-19
Versión del articulo depositado: info:eu-repo/semantics/acceptedVersion
Enlace a la fuente original: https://pubs.acs.org/doi/10.1021/acsami.2c04410
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Acs Applied Materials & Interfaces. 14 (28): 32634-32645
Referencia de l'ítem segons les normes APA: Linklater DP; Le Guével X; Bryant G; Baulin VA; Pereiro E; Perera PGT; Wandiyanto JV; Juodkazis S; Ivanova EP (2022). Lethal Interactions of Atomically Precise Gold Nanoclusters and Pseudomonas aeruginosa and Staphylococcus aureus Bacterial Cells. Acs Applied Materials & Interfaces, 14(28), 32634-32645. DOI: 10.1021/acsami.2c04410
DOI del artículo: 10.1021/acsami.2c04410
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications
Repositori URV