Articles producció científica> Ciències Mèdiques Bàsiques

Opposing Actions of TLR2 and TLR4 in Adipocyte Differentiation and Mature-Onset Obesity

  • Datos identificativos

    Identificador: imarina:9289115
    Autores:
    Cuesta, NataliaFernandez-Veledo, SoniaPunzon, CarmenMoreno, CristobalBarrocal, BeatrizSreeramkumar, VinathaDesco, ManuelFresno, Manuel
    Resumen:
    Understanding the signaling cascades that govern adipocyte metabolism and differentiation is necessary for the development of therapies for obesity. Toll-like receptors (TLRs) are key mediators in adipogenesis, but their specific role is not completely understood. In this study, siRNA knockdown of Tlr2 in 3T3-L1 cells allowed them to differentiate more efficiently into adipocytes, whereas the opposite was observed for the knockdown of Tlr4. At the same time, we show that TLR2 knock-out mice spontaneously developed mature-onset obesity and insulin resistance. Besides a higher incidence of hyperplasia and hypertrophy in white adipose tissue (WAT), we found a significantly increased number of adipocyte precursor cells in TLR2?/? mice compared to TLR4?/? mice. Interestingly, genetic inactivation of Tlr4 in TLR2?/? mice reverted their increased adiposity, insulin resistance, and restored normal levels of adipocyte precursor cells. These findings provide evidence that TLR2 and TLR4 play opposing roles in WAT homeostasis and point to the existence of cross-regulation among TLR2 and TLR4 during adipocyte differentiation both in vitro and in vivo.
  • Otros:

    Autor según el artículo: Cuesta, Natalia; Fernandez-Veledo, Sonia; Punzon, Carmen; Moreno, Cristobal; Barrocal, Beatriz; Sreeramkumar, Vinatha; Desco, Manuel; Fresno, Manuel;
    Departamento: Ciències Mèdiques Bàsiques
    Autor/es de la URV: Fernandez Veledo, Sonia
    Palabras clave: Good health and well-being
    Resumen: Understanding the signaling cascades that govern adipocyte metabolism and differentiation is necessary for the development of therapies for obesity. Toll-like receptors (TLRs) are key mediators in adipogenesis, but their specific role is not completely understood. In this study, siRNA knockdown of Tlr2 in 3T3-L1 cells allowed them to differentiate more efficiently into adipocytes, whereas the opposite was observed for the knockdown of Tlr4. At the same time, we show that TLR2 knock-out mice spontaneously developed mature-onset obesity and insulin resistance. Besides a higher incidence of hyperplasia and hypertrophy in white adipose tissue (WAT), we found a significantly increased number of adipocyte precursor cells in TLR2?/? mice compared to TLR4?/? mice. Interestingly, genetic inactivation of Tlr4 in TLR2?/? mice reverted their increased adiposity, insulin resistance, and restored normal levels of adipocyte precursor cells. These findings provide evidence that TLR2 and TLR4 play opposing roles in WAT homeostasis and point to the existence of cross-regulation among TLR2 and TLR4 during adipocyte differentiation both in vitro and in vivo.
    Áreas temáticas: Zootecnia / recursos pesqueiros Spectroscopy Saúde coletiva Química Psicología Physical and theoretical chemistry Organic chemistry Odontología Nutrição Molecular biology Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Inorganic chemistry Geociências Farmacia Engenharias iv Engenharias ii Engenharias i Educação física Computer science applications Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, multidisciplinary Catalysis Biotecnología Biodiversidade Biochemistry & molecular biology Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: sonia.fernandez@urv.cat
    Identificador del autor: 0000-0003-2906-3788
    Fecha de alta del registro: 2024-11-23
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://mdpi-res.com/d_attachment/ijms/ijms-23-15682/article_deploy/ijms-23-15682-v2.pdf?version=1671672468
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: International Journal Of Molecular Sciences. 23 (24): 15682-15682
    Referencia de l'ítem segons les normes APA: Cuesta, Natalia; Fernandez-Veledo, Sonia; Punzon, Carmen; Moreno, Cristobal; Barrocal, Beatriz; Sreeramkumar, Vinatha; Desco, Manuel; Fresno, Manuel; (2022). Opposing Actions of TLR2 and TLR4 in Adipocyte Differentiation and Mature-Onset Obesity. International Journal Of Molecular Sciences, 23(24), 15682-15682. DOI: 10.3390/ijms232415682
    DOI del artículo: 10.3390/ijms232415682
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2022
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Biochemistry & Molecular Biology,Catalysis,Chemistry, Multidisciplinary,Computer Science Applications,Inorganic Chemistry,Medicine (Miscellaneous),Molecular Biology,Organic Chemistry,Physical and Theoretical Chemistry,Spectroscopy
    Good health and well-being
    Zootecnia / recursos pesqueiros
    Spectroscopy
    Saúde coletiva
    Química
    Psicología
    Physical and theoretical chemistry
    Organic chemistry
    Odontología
    Nutrição
    Molecular biology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    Inorganic chemistry
    Geociências
    Farmacia
    Engenharias iv
    Engenharias ii
    Engenharias i
    Educação física
    Computer science applications
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, multidisciplinary
    Catalysis
    Biotecnología
    Biodiversidade
    Biochemistry & molecular biology
    Astronomia / física
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