Autor según el artículo: Cuesta, Natalia; Fernandez-Veledo, Sonia; Punzon, Carmen; Moreno, Cristobal; Barrocal, Beatriz; Sreeramkumar, Vinatha; Desco, Manuel; Fresno, Manuel;
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: Fernandez Veledo, Sonia
Resumen: Understanding the signaling cascades that govern adipocyte metabolism and differentiation is necessary for the development of therapies for obesity. Toll-like receptors (TLRs) are key mediators in adipogenesis, but their specific role is not completely understood. In this study, siRNA knockdown of Tlr2 in 3T3-L1 cells allowed them to differentiate more efficiently into adipocytes, whereas the opposite was observed for the knockdown of Tlr4. At the same time, we show that TLR2 knock-out mice spontaneously developed mature-onset obesity and insulin resistance. Besides a higher incidence of hyperplasia and hypertrophy in white adipose tissue (WAT), we found a significantly increased number of adipocyte precursor cells in TLR2?/? mice compared to TLR4?/? mice. Interestingly, genetic inactivation of Tlr4 in TLR2?/? mice reverted their increased adiposity, insulin resistance, and restored normal levels of adipocyte precursor cells. These findings provide evidence that TLR2 and TLR4 play opposing roles in WAT homeostasis and point to the existence of cross-regulation among TLR2 and TLR4 during adipocyte differentiation both in vitro and in vivo.
Áreas temáticas: Astronomia / física Biochemistry & molecular biology Biodiversidade Biotecnología Catalysis Chemistry, multidisciplinary Ciência da computação Ciência de alimentos Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Computer science applications Educação física Engenharias i Engenharias ii Engenharias iv Farmacia Geociências Inorganic chemistry Interdisciplinar Materiais Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (miscellaneous) Molecular biology Nutrição Odontología Organic chemistry Physical and theoretical chemistry Psicología Química Saúde coletiva Spectroscopy Zootecnia / recursos pesqueiros
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: sonia.fernandez@urv.cat
Identificador del autor: 0000-0003-2906-3788
Fecha de alta del registro: 2023-02-19
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://mdpi-res.com/d_attachment/ijms/ijms-23-15682/article_deploy/ijms-23-15682-v2.pdf?version=1671672468
Referencia al articulo segun fuente origial: International Journal Of Molecular Sciences. 23 (24): 15682-
Referencia de l'ítem segons les normes APA: Cuesta, Natalia; Fernandez-Veledo, Sonia; Punzon, Carmen; Moreno, Cristobal; Barrocal, Beatriz; Sreeramkumar, Vinatha; Desco, Manuel; Fresno, Manuel; (2022). Opposing Actions of TLR2 and TLR4 in Adipocyte Differentiation and Mature-Onset Obesity. International Journal Of Molecular Sciences, 23(24), 15682-. DOI: 10.3390/ijms232415682
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.3390/ijms232415682
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2022
Tipo de publicación: Journal Publications