Autor según el artículo: Robert, Alexandre; Talagrand-Reboul, Emilie; Figueras, Maria-Jose; Ruimy, Raymond; Boyer, Laurent; Lamy, Brigitte;
Departamento: Ciències Mèdiques Bàsiques
Autor/es de la URV: Figueras Salvat, María Josefa
Palabras clave: Aeromonas Bacteria Disease Drosophila melanogaster Host Infection model Lessons Microbial interference Opportunistic pathogen Polymicrobial infection Pseudomonas-aeruginosa
Resumen: Background: Polymicrobial infections are complex infections associated with worse outcomes compared to monomicrobial infections. We need simple, fast, and cost-effective animal models to assess their still poorly known pathogenesis. Methods: We developed a Drosophila melanogaster polymicrobial infection model for opportunistic pathogens and assessed its capacity to discriminate the effects of bacterial mixtures taken from cases of human polymicrobial infections by Aeromonas strains. A systemic infection was obtained by needle pricking the dorsal thorax of the flies, and the fly survival was monitored over time. Different lineages of the flies were infected by a single strain or paired strains (strain ratio 1:1). Results: Individual strains killed more than 80% of the flies in 20 h. The course of infection could be altered with a microbial mix. The model could distinguish between the diverse effects (synergistic, antagonistic, and no difference) that resulted in a milder, more severe, or similar infection, depending on the paired strain considered. We then investigated the determinants of the effects. The effects were maintained in deficient fly lineages for the main signaling pathways (Toll deficient and IMD deficient), which suggests an active microbe/microbe/host interaction. Conclusion: These results indicate that the D. melanogaster systemic infection model is consistent with the study of polymicrobial infection.
Áreas temáticas: Farmacia General immunology and microbiology Immunology and allergy Immunology and microbiology (all) Immunology and microbiology (miscellaneous) Infectious diseases Microbiology Microbiology (medical) Molecular biology
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: mariajose.figueras@urv.cat
Identificador del autor: 0000-0002-2268-8980
Fecha de alta del registro: 2023-04-22
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.mdpi.com/2076-0817/12/3/405
Referencia al articulo segun fuente origial: Pathogens. 12 (3):
Referencia de l'ítem segons les normes APA: Robert, Alexandre; Talagrand-Reboul, Emilie; Figueras, Maria-Jose; Ruimy, Raymond; Boyer, Laurent; Lamy, Brigitte; (2023). Drosophila melanogaster Systemic Infection Model to Study Altered Virulence during Polymicrobial Infection by Aeromonas. Pathogens, 12(3), -. DOI: 10.3390/pathogens12030405
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.3390/pathogens12030405
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications
Repositori URV