Autor según el artículo: Ribes, Angela; Xifre-Perez, Elisabet; Aznar, Elena; Sancenon, Felix; Pardo, Teresa; Marsal, Lluis F.; Martinez-Manez, Ramon;
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Marsal Garví, Luis Francisco / Xifré Pérez, Elisabet
Palabras clave: Systems Specificity Responsive drug-delivery Platform Oral fluid Mesoporous silica nanoparticles Dna Bioresponsive controlled-release Aptasensor Aptamer-based sensor
Resumen: We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement "molecular gates" for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked by the subsequent hybridisation of a specific cocaine aptamer. The response of the gated material was studied in aqueous solution. In a typical experiment, the support was immersed in hybridisation buffer solution in the absence or presence of cocaine. At certain times, the release of rhodamine B from pore voids was measured by fluorescence spectroscopy. The capped NAA support showed poor cargo delivery, but presence of cocaine in the solution selectively induced rhodamine B release. By this simple procedure a limit of detection as low as 5 x 10(-7) M was calculated for cocaine. The gated NAA was successfully applied to detect cocaine in saliva samples and the possible re-use of the nanostructures was assessed. Based on these results, we believe that NAA could be a suitable support to prepare optical gated probes with a synergic combination of the favourable features of selected gated sensing systems and NAA.
Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: lluis.marsal@urv.cat elisabet.xifre@urv.cat
Identificador del autor: 0000-0002-5976-1408 0000-0001-6072-9889
Fecha de alta del registro: 2023-05-20
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://www.nature.com/articles/srep38649
Referencia al articulo segun fuente origial: Scientific Reports. 6
Referencia de l'ítem segons les normes APA: Ribes, Angela; Xifre-Perez, Elisabet; Aznar, Elena; Sancenon, Felix; Pardo, Teresa; Marsal, Lluis F.; Martinez-Manez, Ramon; (2016). Molecular gated nanoporous anodic alumina for the detection of cocaine. Scientific Reports, 6(), -. DOI: 10.1038/srep38649
URL Documento de licencia: http://repositori.urv.cat/ca/proteccio-de-dades/
DOI del artículo: 10.1038/srep38649
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2016
Tipo de publicación: Journal Publications
Repositori URV