Articles producció científica> Enginyeria Electrònica, Elèctrica i Automàtica

Molecular gated nanoporous anodic alumina for the detection of cocaine

  • Datos identificativos

    Identificador: imarina:9297462
    Autores:
    Ribes, AngelaAznar, ElenaSancenon, FelixPardo, TeresaMarsal, Lluis FMartinez-Manez, Ramon
    Resumen:
    We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement "molecular gates" for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked by the subsequent hybridisation of a specific cocaine aptamer. The response of the gated material was studied in aqueous solution. In a typical experiment, the support was immersed in hybridisation buffer solution in the absence or presence of cocaine. At certain times, the release of rhodamine B from pore voids was measured by fluorescence spectroscopy. The capped NAA support showed poor cargo delivery, but presence of cocaine in the solution selectively induced rhodamine B release. By this simple procedure a limit of detection as low as 5 x 10(-7) M was calculated for cocaine. The gated NAA was successfully applied to detect cocaine in saliva samples and the possible re-use of the nanostructures was assessed. Based on these results, we believe that NAA could be a suitable support to prepare optical gated probes with a synergic combination of the favourable features of selected gated sensing systems and NAA.
  • Otros:

    Autor según el artículo: Ribes, Angela; Aznar, Elena; Sancenon, Felix; Pardo, Teresa; Marsal, Lluis F; Martinez-Manez, Ramon
    Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
    Autor/es de la URV: Marsal Garví, Luis Francisco / Xifré Pérez, Elisabet
    Palabras clave: Systems Specificity Responsive drug-delivery Platform Oral fluid Mesoporous silica nanoparticles Dna Bioresponsive controlled-release Aptasensor Aptamer-based sensor
    Resumen: We present herein the use of nanoporous anodic alumina (NAA) as a suitable support to implement "molecular gates" for sensing applications. In our design, a NAA support is loaded with a fluorescent reporter (rhodamine B) and functionalized with a short single-stranded DNA. Then pores are blocked by the subsequent hybridisation of a specific cocaine aptamer. The response of the gated material was studied in aqueous solution. In a typical experiment, the support was immersed in hybridisation buffer solution in the absence or presence of cocaine. At certain times, the release of rhodamine B from pore voids was measured by fluorescence spectroscopy. The capped NAA support showed poor cargo delivery, but presence of cocaine in the solution selectively induced rhodamine B release. By this simple procedure a limit of detection as low as 5 x 10(-7) M was calculated for cocaine. The gated NAA was successfully applied to detect cocaine in saliva samples and the possible re-use of the nanostructures was assessed. Based on these results, we believe that NAA could be a suitable support to prepare optical gated probes with a synergic combination of the favourable features of selected gated sensing systems and NAA.
    Áreas temáticas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: elisabet.xifre@urv.cat lluis.marsal@urv.cat
    Identificador del autor: 0000-0001-6072-9889 0000-0002-5976-1408
    Fecha de alta del registro: 2024-10-12
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://www.nature.com/articles/srep38649
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Scientific Reports. 6 38649-
    Referencia de l'ítem segons les normes APA: Ribes, Angela; Aznar, Elena; Sancenon, Felix; Pardo, Teresa; Marsal, Lluis F; Martinez-Manez, Ramon (2016). Molecular gated nanoporous anodic alumina for the detection of cocaine. Scientific Reports, 6(), 38649-. DOI: 10.1038/srep38649
    DOI del artículo: 10.1038/srep38649
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2016
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Multidisciplinary,Multidisciplinary Sciences
    Systems
    Specificity
    Responsive drug-delivery
    Platform
    Oral fluid
    Mesoporous silica nanoparticles
    Dna
    Bioresponsive controlled-release
    Aptasensor
    Aptamer-based sensor
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Psicología
    Odontología
    Nutrição
    Multidisciplinary sciences
    Multidisciplinary
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Letras / linguística
    Interdisciplinar
    Geografía
    Geociências
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Enfermagem
    Educação física
    Educação
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
    Astronomia / física
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