Autor según el artículo: Dabare, Panthihage Ruvini L; Bachhuka, Akash; Quek, Jing Yang; Marsal, Lluis F F; Hayball, John; Vasilev, Krasimir
Departamento: Enginyeria Electrònica, Elèctrica i Automàtica
Autor/es de la URV: Bachhuka, Akash / Marsal Garví, Luis Francisco
Palabras clave: Topography Surface nanotopography Plasma polymerization Nanotopography Modulation Macrophage response Macrophage polarization Interleukin-10 Fusion Foreign body reaction Differentiation Chemistry Body giant-cells Biomaterials Activation
Resumen: Macrophage polarization is a significant event in the host immune response, which can be modulated by modifying the surface of a biomaterial. Previous studies have demonstrated the modulation of macrophage polarization using different surface features; however, none of these studies reflect the effect of surface properties on unstimulated macrophage polarization for a prolonged period. To better understand the impact of surface features, in this work differentiated THP-1 cells are employed to control macrophage polarization on nano-rough surfaces for a duration of 7 days. Model nano-rough substrates are fabricated by immobilizing gold nanoparticles (AuNPs) of predetermined sizes (16, 38, 68 nm) on a 2-methyl-2-oxazoline thin film, followed by tailoring the outermost surface chemistry. All modified surfaces support high levels of cell adhesion and proliferation. Over time, the expression of pro-inflammatory cytokines decreases, whereas the expression of anti-inflammatory cytokines increases on all modified surfaces. Similarly, pro-inflammatory interleukin (IL)-1 & beta; gene expression is downregulated, and anti-inflammatory IL-10-gene expression is upregulated, regardless of the surface roughness. Analysis of cell morphology reveals that the predominant cell type on the modified surfaces exhibits M2 anti-inflammatory phenotype. Herein, how surface features can modulate macrophage responses over an extended period is highlighted, offering insights for the development of future biomaterial implants.
Áreas temáticas: Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Chemical engineering (miscellaneous) Catalysis
Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
Direcció de correo del autor: akash.bachhuka@urv.cat lluis.marsal@urv.cat
Identificador del autor: 0000-0003-1253-8126 0000-0002-5976-1408
Fecha de alta del registro: 2024-10-12
Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
Enlace a la fuente original: https://onlinelibrary.wiley.com/doi/full/10.1002/smsc.202300080
URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
Referencia al articulo segun fuente origial: Small Science. 3 (10):
Referencia de l'ítem segons les normes APA: Dabare, Panthihage Ruvini L; Bachhuka, Akash; Quek, Jing Yang; Marsal, Lluis F F; Hayball, John; Vasilev, Krasimir (2023). Nano-Roughness-Mediated Macrophage Polarization for Desired Host Immune Response. Small Science, 3(10), -. DOI: 10.1002/smsc.202300080
DOI del artículo: 10.1002/smsc.202300080
Entidad: Universitat Rovira i Virgili
Año de publicación de la revista: 2023
Tipo de publicación: Journal Publications