Articles producció científica> Química Física i Inorgànica

Computational and experimental microfluidics: Total analysis system for mixing, sorting, and concentrating particles and cells

  • Datos identificativos

    Identificador: imarina:9366240
    Autores:
    Coral, DavidAttard, MatthewPedrol, EricSole, Rosa MariaDiaz, FrancescAguilo, MagdalenaMateos, Xavier
    Resumen:
    Body fluids can potentially indicate the presence of non-small cancer cells. Studying these fluids is an emerging field that could be crucial for cancer detection and monitoring treatment effectiveness. Meanwhile, the examination of fluids on a microscopic level is part of the field of microfluidics. This study focuses on the development of a total analysis system that consists of various interconnected structures that are designed to mix, classify, concentrate, and isolate particles in fluids that mimic the behavior of cancer and normal cells. Using the COMSOL Multiphysics software, the device's performance was optimized to use a pressure input of 35 kPa for water or serum and 29.4 kPa for a mixture of liquid and serum samples, which are the optimal pressure inputs. The numerical models were validated by experiments using two types of polystyrene particles, with diameters of 5 and 20 mu m. Moreover, the developed system was applied to monitor the behavior of red blood cells. The microfluidic chip is capable of addressing several challenges through visual detections, including mixing tests of two fluids with similar densities, proper particle size classification using Dean flow fractionation, and single-step recovery of large, labeled particles. Finally, the collected particles were examined using an environmental scanning electron microscope to determine their size, and the results demonstrated that successful size separation was achieved, with particles around 20 mu m completely separated from the smaller ones.
  • Otros:

    Autor según el artículo: Coral, David; Attard, Matthew; Pedrol, Eric; Sole, Rosa Maria; Diaz, Francesc; Aguilo, Magdalena; Mateos, Xavier
    Departamento: Química Física i Inorgànica
    Autor/es de la URV: Aguiló Díaz, Magdalena / Díaz González, Francisco Manuel / Mateos Ferré, Xavier / Solé Cartañà, Rosa Maria
    Resumen: Body fluids can potentially indicate the presence of non-small cancer cells. Studying these fluids is an emerging field that could be crucial for cancer detection and monitoring treatment effectiveness. Meanwhile, the examination of fluids on a microscopic level is part of the field of microfluidics. This study focuses on the development of a total analysis system that consists of various interconnected structures that are designed to mix, classify, concentrate, and isolate particles in fluids that mimic the behavior of cancer and normal cells. Using the COMSOL Multiphysics software, the device's performance was optimized to use a pressure input of 35 kPa for water or serum and 29.4 kPa for a mixture of liquid and serum samples, which are the optimal pressure inputs. The numerical models were validated by experiments using two types of polystyrene particles, with diameters of 5 and 20 mu m. Moreover, the developed system was applied to monitor the behavior of red blood cells. The microfluidic chip is capable of addressing several challenges through visual detections, including mixing tests of two fluids with similar densities, proper particle size classification using Dean flow fractionation, and single-step recovery of large, labeled particles. Finally, the collected particles were examined using an environmental scanning electron microscope to determine their size, and the results demonstrated that successful size separation was achieved, with particles around 20 mu m completely separated from the smaller ones.
    Áreas temáticas: Engineering, biomedical Biophysics Biomedical engineering Biomaterials Bioengineering
    Acceso a la licencia de uso: https://creativecommons.org/licenses/by/3.0/es/
    Direcció de correo del autor: f.diaz@urv.cat magdalena.aguilo@urv.cat rosam.sole@urv.cat xavier.mateos@urv.cat
    Identificador del autor: 0000-0003-4581-4967 0000-0001-6130-9579 0000-0002-5769-4141 0000-0003-1940-1990
    Fecha de alta del registro: 2024-08-03
    Versión del articulo depositado: info:eu-repo/semantics/publishedVersion
    Enlace a la fuente original: https://pubs.aip.org/aip/apb/article/8/2/026101/3283008/Computational-and-experimental-microfluidics-Total
    URL Documento de licencia: https://repositori.urv.cat/ca/proteccio-de-dades/
    Referencia al articulo segun fuente origial: Apl Bioengineering. 8 (2): 026101-
    Referencia de l'ítem segons les normes APA: Coral, David; Attard, Matthew; Pedrol, Eric; Sole, Rosa Maria; Diaz, Francesc; Aguilo, Magdalena; Mateos, Xavier (2024). Computational and experimental microfluidics: Total analysis system for mixing, sorting, and concentrating particles and cells. Apl Bioengineering, 8(2), 026101-. DOI: 10.1063/5.0158648
    DOI del artículo: 10.1063/5.0158648
    Entidad: Universitat Rovira i Virgili
    Año de publicación de la revista: 2024
    Tipo de publicación: Journal Publications
  • Palabras clave:

    Bioengineering,Biomaterials,Biomedical Engineering,Biophysics,Engineering, Biomedical
    Engineering, biomedical
    Biophysics
    Biomedical engineering
    Biomaterials
    Bioengineering
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